Natural Killer T Cell Ligand α‐Galactosylceramide Inhibited Lymph Node Metastasis of Highly Metastatic Melanoma Cells

The role of natural killer T (NKT) cells in the prevention of multiple tumor metastasis was examined. The i.v. inoculation of a highly metastatic subline of B16‐BL6 (B16‐BL6‐HM) melanoma cells resulted in the formation of metastatic nodules in lymph nodes in addition to lung, intrapleural cavity, an...

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Autores principales: Nakui, Minoru, Iwakabe, Kenji, Ohta, Akio, Sekimoto, Masashi, Sato, Marimo, Makuuchi, Hiroyasu, Kawano, Tetsu, Taniguchi, Masaru, Nishimura, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1999
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926145/
https://www.ncbi.nlm.nih.gov/pubmed/10543249
http://dx.doi.org/10.1111/j.1349-7006.1999.tb00818.x
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author Nakui, Minoru
Iwakabe, Kenji
Ohta, Akio
Sekimoto, Masashi
Sato, Marimo
Makuuchi, Hiroyasu
Kawano, Tetsu
Taniguchi, Masaru
Nishimura, Takashi
author_facet Nakui, Minoru
Iwakabe, Kenji
Ohta, Akio
Sekimoto, Masashi
Sato, Marimo
Makuuchi, Hiroyasu
Kawano, Tetsu
Taniguchi, Masaru
Nishimura, Takashi
author_sort Nakui, Minoru
collection PubMed
description The role of natural killer T (NKT) cells in the prevention of multiple tumor metastasis was examined. The i.v. inoculation of a highly metastatic subline of B16‐BL6 (B16‐BL6‐HM) melanoma cells resulted in the formation of metastatic nodules in lymph nodes in addition to lung, intrapleural cavity, and ovary. However, treatment of the mice with the NKT cell ligand α‐galactosylceramide (α‐GalCer) three times from 1 day after B16‐BL6‐HM melanoma inoculation caused a significant inhibition of multiple metastasis. Lymph node metastasis of B16‐BL6‐HM was almost completely blocked by α‐GalCer treatment. This antimetastatic effect of α‐GalCer was abolished in NKT celldeficient mice. These results suggest that α‐GalCer‐activated NKT cells played a critical role in the prevention of lymph node metastasis of melanoma cells.
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spelling pubmed-59261452018-05-11 Natural Killer T Cell Ligand α‐Galactosylceramide Inhibited Lymph Node Metastasis of Highly Metastatic Melanoma Cells Nakui, Minoru Iwakabe, Kenji Ohta, Akio Sekimoto, Masashi Sato, Marimo Makuuchi, Hiroyasu Kawano, Tetsu Taniguchi, Masaru Nishimura, Takashi Jpn J Cancer Res Article The role of natural killer T (NKT) cells in the prevention of multiple tumor metastasis was examined. The i.v. inoculation of a highly metastatic subline of B16‐BL6 (B16‐BL6‐HM) melanoma cells resulted in the formation of metastatic nodules in lymph nodes in addition to lung, intrapleural cavity, and ovary. However, treatment of the mice with the NKT cell ligand α‐galactosylceramide (α‐GalCer) three times from 1 day after B16‐BL6‐HM melanoma inoculation caused a significant inhibition of multiple metastasis. Lymph node metastasis of B16‐BL6‐HM was almost completely blocked by α‐GalCer treatment. This antimetastatic effect of α‐GalCer was abolished in NKT celldeficient mice. These results suggest that α‐GalCer‐activated NKT cells played a critical role in the prevention of lymph node metastasis of melanoma cells. Blackwell Publishing Ltd 1999-08 /pmc/articles/PMC5926145/ /pubmed/10543249 http://dx.doi.org/10.1111/j.1349-7006.1999.tb00818.x Text en
spellingShingle Article
Nakui, Minoru
Iwakabe, Kenji
Ohta, Akio
Sekimoto, Masashi
Sato, Marimo
Makuuchi, Hiroyasu
Kawano, Tetsu
Taniguchi, Masaru
Nishimura, Takashi
Natural Killer T Cell Ligand α‐Galactosylceramide Inhibited Lymph Node Metastasis of Highly Metastatic Melanoma Cells
title Natural Killer T Cell Ligand α‐Galactosylceramide Inhibited Lymph Node Metastasis of Highly Metastatic Melanoma Cells
title_full Natural Killer T Cell Ligand α‐Galactosylceramide Inhibited Lymph Node Metastasis of Highly Metastatic Melanoma Cells
title_fullStr Natural Killer T Cell Ligand α‐Galactosylceramide Inhibited Lymph Node Metastasis of Highly Metastatic Melanoma Cells
title_full_unstemmed Natural Killer T Cell Ligand α‐Galactosylceramide Inhibited Lymph Node Metastasis of Highly Metastatic Melanoma Cells
title_short Natural Killer T Cell Ligand α‐Galactosylceramide Inhibited Lymph Node Metastasis of Highly Metastatic Melanoma Cells
title_sort natural killer t cell ligand α‐galactosylceramide inhibited lymph node metastasis of highly metastatic melanoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926145/
https://www.ncbi.nlm.nih.gov/pubmed/10543249
http://dx.doi.org/10.1111/j.1349-7006.1999.tb00818.x
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