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Increased Serum Levels of Vascular Endothelial Growth Factor in Patients with Renal Cell Carcinoma
Neovascularization, an essential event for the growth of solid tumors, is regulated by a number of angiogenic factors. One such factor, vascular endothelial growth factor (VEGF), is considered to exert a potent angiogenic activity, as indicated by immunohistochemical and molecular evidence. In this...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926146/ https://www.ncbi.nlm.nih.gov/pubmed/10543260 http://dx.doi.org/10.1111/j.1349-7006.1999.tb00829.x |
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author | Sato, Kazunari Tsuchiya, Norihiko Sasaki, Ryusei Shimoda, Naotake Satoh, Shigeru Ogawa, Osamu Kato, Tetsuro |
author_facet | Sato, Kazunari Tsuchiya, Norihiko Sasaki, Ryusei Shimoda, Naotake Satoh, Shigeru Ogawa, Osamu Kato, Tetsuro |
author_sort | Sato, Kazunari |
collection | PubMed |
description | Neovascularization, an essential event for the growth of solid tumors, is regulated by a number of angiogenic factors. One such factor, vascular endothelial growth factor (VEGF), is considered to exert a potent angiogenic activity, as indicated by immunohistochemical and molecular evidence. In this study we investigated the serum VEGF level (s‐VEGF) in patients with renal cell carcinoma (RCC). s‐VEGF in peripheral blood samples was analyzed in 40 RCC patients and 40 patients without cancer (controls) using a sandwich enzyme‐linked immunoassay. In 20 RCC patients, serum samples were obtained separately from the bilateral renal veins. s‐VEGF was also measured before, 4 and 8 weeks after nephrectomy in 11 patients. There were significant differences in s‐VEGF between the RCC patients and the controls (207.3 ± 32.9 vs. 71.5 ± 9.1 pg/ml, mean ± SE) (P < 0.005), between the tumor‐bearing renal veins and the contralateral ones (P < 0.01), between the pre‐ and post‐nephrectomy situations (P < 0.01) and among the various parameters of tumor status such as tumor extent (P < 0.001) and existence of metastasis (P < 0.001). s‐VEGF significantly correlated with the tumor volume obtained by a three‐dimensional measurement (r= 0.802, P < 0.0001). The sensitivity and specificity of s‐VEGF at the cut‐off level of 100 pg/ml, as determined by the receiver‐operating‐characteristics curve, were 80.0% and 72.5%, respectively. The results indicate that tumor tissue of RCC liberates VEGF into the systemic blood flow and that s‐VEGF is a possible marker for RCC. |
format | Online Article Text |
id | pubmed-5926146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59261462018-05-11 Increased Serum Levels of Vascular Endothelial Growth Factor in Patients with Renal Cell Carcinoma Sato, Kazunari Tsuchiya, Norihiko Sasaki, Ryusei Shimoda, Naotake Satoh, Shigeru Ogawa, Osamu Kato, Tetsuro Jpn J Cancer Res Article Neovascularization, an essential event for the growth of solid tumors, is regulated by a number of angiogenic factors. One such factor, vascular endothelial growth factor (VEGF), is considered to exert a potent angiogenic activity, as indicated by immunohistochemical and molecular evidence. In this study we investigated the serum VEGF level (s‐VEGF) in patients with renal cell carcinoma (RCC). s‐VEGF in peripheral blood samples was analyzed in 40 RCC patients and 40 patients without cancer (controls) using a sandwich enzyme‐linked immunoassay. In 20 RCC patients, serum samples were obtained separately from the bilateral renal veins. s‐VEGF was also measured before, 4 and 8 weeks after nephrectomy in 11 patients. There were significant differences in s‐VEGF between the RCC patients and the controls (207.3 ± 32.9 vs. 71.5 ± 9.1 pg/ml, mean ± SE) (P < 0.005), between the tumor‐bearing renal veins and the contralateral ones (P < 0.01), between the pre‐ and post‐nephrectomy situations (P < 0.01) and among the various parameters of tumor status such as tumor extent (P < 0.001) and existence of metastasis (P < 0.001). s‐VEGF significantly correlated with the tumor volume obtained by a three‐dimensional measurement (r= 0.802, P < 0.0001). The sensitivity and specificity of s‐VEGF at the cut‐off level of 100 pg/ml, as determined by the receiver‐operating‐characteristics curve, were 80.0% and 72.5%, respectively. The results indicate that tumor tissue of RCC liberates VEGF into the systemic blood flow and that s‐VEGF is a possible marker for RCC. Blackwell Publishing Ltd 1999-08 /pmc/articles/PMC5926146/ /pubmed/10543260 http://dx.doi.org/10.1111/j.1349-7006.1999.tb00829.x Text en |
spellingShingle | Article Sato, Kazunari Tsuchiya, Norihiko Sasaki, Ryusei Shimoda, Naotake Satoh, Shigeru Ogawa, Osamu Kato, Tetsuro Increased Serum Levels of Vascular Endothelial Growth Factor in Patients with Renal Cell Carcinoma |
title | Increased Serum Levels of Vascular Endothelial Growth Factor in Patients with Renal Cell Carcinoma |
title_full | Increased Serum Levels of Vascular Endothelial Growth Factor in Patients with Renal Cell Carcinoma |
title_fullStr | Increased Serum Levels of Vascular Endothelial Growth Factor in Patients with Renal Cell Carcinoma |
title_full_unstemmed | Increased Serum Levels of Vascular Endothelial Growth Factor in Patients with Renal Cell Carcinoma |
title_short | Increased Serum Levels of Vascular Endothelial Growth Factor in Patients with Renal Cell Carcinoma |
title_sort | increased serum levels of vascular endothelial growth factor in patients with renal cell carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926146/ https://www.ncbi.nlm.nih.gov/pubmed/10543260 http://dx.doi.org/10.1111/j.1349-7006.1999.tb00829.x |
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