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Annexin VII as a Novel Marker for Invasive Phenotype of Malignant Melanoma

Both F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. While examining the genetic difference between the two sublines, we found a marked reduction of annexin VII expression in BL6 cells. In ad...

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Autores principales: Kataoka, Tatsuki R., Ito, Akihiko, Asada, Hideo, Watabe, Kenji, Nishiyama, Kazutaka, Nakamoto, Ken'i, Itami, Satoshi, Yoshikawa, Kunihiko, Ito, Masaki, Nojima, Hiroshi, Kitamura, Yukihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926233/
https://www.ncbi.nlm.nih.gov/pubmed/10744047
http://dx.doi.org/10.1111/j.1349-7006.2000.tb00862.x
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author Kataoka, Tatsuki R.
Ito, Akihiko
Asada, Hideo
Watabe, Kenji
Nishiyama, Kazutaka
Nakamoto, Ken'i
Itami, Satoshi
Yoshikawa, Kunihiko
Ito, Masaki
Nojima, Hiroshi
Kitamura, Yukihiko
author_facet Kataoka, Tatsuki R.
Ito, Akihiko
Asada, Hideo
Watabe, Kenji
Nishiyama, Kazutaka
Nakamoto, Ken'i
Itami, Satoshi
Yoshikawa, Kunihiko
Ito, Masaki
Nojima, Hiroshi
Kitamura, Yukihiko
author_sort Kataoka, Tatsuki R.
collection PubMed
description Both F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. While examining the genetic difference between the two sublines, we found a marked reduction of annexin VII expression in BL6 cells. In addition, fusion cell clones of both sublines, were as poorly metastatic as F10 cells after subcutaneous injection, and contained the annexin VII message as abundantly as F10 cells. Hence, we examined whether the annexin VII expression was correlated with the less malignant phenotype of clinical cases by immunohistochemistry. Immunoreactivities to anti‐annexin VII antibody in melanoma cells were evaluated quantitatively by using skin mast cells as an internal positive control. Eighteen patients with malignant melanoma were divided into two groups: lymph node metastasis‐negative and positive groups. The ratio of numbers of patients positive versus negative to the antibody was significantly larger in the former than in the latter group. These results not only indicated that annexin VII serves as a marker for less invasive phenotype of malignant melanoma, but also suggested a possible role of annexin VII in tumor suppression.
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spelling pubmed-59262332018-05-11 Annexin VII as a Novel Marker for Invasive Phenotype of Malignant Melanoma Kataoka, Tatsuki R. Ito, Akihiko Asada, Hideo Watabe, Kenji Nishiyama, Kazutaka Nakamoto, Ken'i Itami, Satoshi Yoshikawa, Kunihiko Ito, Masaki Nojima, Hiroshi Kitamura, Yukihiko Jpn J Cancer Res Article Both F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. While examining the genetic difference between the two sublines, we found a marked reduction of annexin VII expression in BL6 cells. In addition, fusion cell clones of both sublines, were as poorly metastatic as F10 cells after subcutaneous injection, and contained the annexin VII message as abundantly as F10 cells. Hence, we examined whether the annexin VII expression was correlated with the less malignant phenotype of clinical cases by immunohistochemistry. Immunoreactivities to anti‐annexin VII antibody in melanoma cells were evaluated quantitatively by using skin mast cells as an internal positive control. Eighteen patients with malignant melanoma were divided into two groups: lymph node metastasis‐negative and positive groups. The ratio of numbers of patients positive versus negative to the antibody was significantly larger in the former than in the latter group. These results not only indicated that annexin VII serves as a marker for less invasive phenotype of malignant melanoma, but also suggested a possible role of annexin VII in tumor suppression. Blackwell Publishing Ltd 2000-01 /pmc/articles/PMC5926233/ /pubmed/10744047 http://dx.doi.org/10.1111/j.1349-7006.2000.tb00862.x Text en
spellingShingle Article
Kataoka, Tatsuki R.
Ito, Akihiko
Asada, Hideo
Watabe, Kenji
Nishiyama, Kazutaka
Nakamoto, Ken'i
Itami, Satoshi
Yoshikawa, Kunihiko
Ito, Masaki
Nojima, Hiroshi
Kitamura, Yukihiko
Annexin VII as a Novel Marker for Invasive Phenotype of Malignant Melanoma
title Annexin VII as a Novel Marker for Invasive Phenotype of Malignant Melanoma
title_full Annexin VII as a Novel Marker for Invasive Phenotype of Malignant Melanoma
title_fullStr Annexin VII as a Novel Marker for Invasive Phenotype of Malignant Melanoma
title_full_unstemmed Annexin VII as a Novel Marker for Invasive Phenotype of Malignant Melanoma
title_short Annexin VII as a Novel Marker for Invasive Phenotype of Malignant Melanoma
title_sort annexin vii as a novel marker for invasive phenotype of malignant melanoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926233/
https://www.ncbi.nlm.nih.gov/pubmed/10744047
http://dx.doi.org/10.1111/j.1349-7006.2000.tb00862.x
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