Cargando…

The Characteristics of Epstein‐Barr Virus (EBV)‐positive Diffuse Large B‐Cell Lymphoma: Comparison between EBV(+) and EBV(‐) Cases in Japanese Population

We have investigated 114 cases with diffuse large B‐cell lymphoma (DLBCL) to clarify the characteristics of DLBCL with Epstein‐Barr virus (EBV) infection. Thirteen cases (11.4%) showed EBVencoded RNA 1 (EBER1) signals by RNA in situ hybridization. EBV‐encoded latent membrane protein 1 (LMP1) and EBV...

Descripción completa

Detalles Bibliográficos
Autores principales: Kuze, Tetuo, Nakamura, Naoya, Hashimoto, Yuko, Sasaki, Yoshikazu, Abe, Masafumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926305/
https://www.ncbi.nlm.nih.gov/pubmed/11123421
http://dx.doi.org/10.1111/j.1349-7006.2000.tb00909.x
_version_ 1783318875243282432
author Kuze, Tetuo
Nakamura, Naoya
Hashimoto, Yuko
Sasaki, Yoshikazu
Abe, Masafumi
author_facet Kuze, Tetuo
Nakamura, Naoya
Hashimoto, Yuko
Sasaki, Yoshikazu
Abe, Masafumi
author_sort Kuze, Tetuo
collection PubMed
description We have investigated 114 cases with diffuse large B‐cell lymphoma (DLBCL) to clarify the characteristics of DLBCL with Epstein‐Barr virus (EBV) infection. Thirteen cases (11.4%) showed EBVencoded RNA 1 (EBER1) signals by RNA in situ hybridization. EBV‐encoded latent membrane protein 1 (LMP1) and EBV‐encoded nuclear antigen 2 (EBNA2) were expressed in 11 and 4 cases, respectively. Expression of CD30, Bcl‐6 and immunoglobulin (Ig) was found in 92%, 31% and 23% with EBV(+) DLBCL, and in 15%, 79% and 82% with EBV(‐) DLBCL, respectively. The sequence of rearranged Ig heavy chain (IgH) variable (V) region gene was analyzed in 5 cases with EBV(+) DLBCL and 61 cases with EBV(‐) DLBCL. Somatic mutation was found in all cases except one with EBV(‐) DLBCL. Average mutation frequency was 9.6% in EBV(+) DLBCL vs. 11.5% in EBV(‐) DLBCL. The rates of replacement mutation vs. silent mutation (R/S values) in complementarity determining region II and framework region III were 2.7 and 1.5 in EBV(+) DLBCL, 2.6 and 1.4 in EBV(‐) DLBCL. Crippling mutation generating a stop codon was found in 2 of 5 cases (40%) with EBV(+) DLBCL, but none of 61 cases (0%) with EBV(‐) DLBCL. These findings suggest that EBV(+) DLBCL and EBV(‐) DLBCL were both derived from germinal center (GC) or post‐GCB cells, and EBV(+) DLBCL frequently have a non‐functional IgH gene owing to crippling mutation.
format Online
Article
Text
id pubmed-5926305
institution National Center for Biotechnology Information
language English
publishDate 2000
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59263052018-05-11 The Characteristics of Epstein‐Barr Virus (EBV)‐positive Diffuse Large B‐Cell Lymphoma: Comparison between EBV(+) and EBV(‐) Cases in Japanese Population Kuze, Tetuo Nakamura, Naoya Hashimoto, Yuko Sasaki, Yoshikazu Abe, Masafumi Jpn J Cancer Res Article We have investigated 114 cases with diffuse large B‐cell lymphoma (DLBCL) to clarify the characteristics of DLBCL with Epstein‐Barr virus (EBV) infection. Thirteen cases (11.4%) showed EBVencoded RNA 1 (EBER1) signals by RNA in situ hybridization. EBV‐encoded latent membrane protein 1 (LMP1) and EBV‐encoded nuclear antigen 2 (EBNA2) were expressed in 11 and 4 cases, respectively. Expression of CD30, Bcl‐6 and immunoglobulin (Ig) was found in 92%, 31% and 23% with EBV(+) DLBCL, and in 15%, 79% and 82% with EBV(‐) DLBCL, respectively. The sequence of rearranged Ig heavy chain (IgH) variable (V) region gene was analyzed in 5 cases with EBV(+) DLBCL and 61 cases with EBV(‐) DLBCL. Somatic mutation was found in all cases except one with EBV(‐) DLBCL. Average mutation frequency was 9.6% in EBV(+) DLBCL vs. 11.5% in EBV(‐) DLBCL. The rates of replacement mutation vs. silent mutation (R/S values) in complementarity determining region II and framework region III were 2.7 and 1.5 in EBV(+) DLBCL, 2.6 and 1.4 in EBV(‐) DLBCL. Crippling mutation generating a stop codon was found in 2 of 5 cases (40%) with EBV(+) DLBCL, but none of 61 cases (0%) with EBV(‐) DLBCL. These findings suggest that EBV(+) DLBCL and EBV(‐) DLBCL were both derived from germinal center (GC) or post‐GCB cells, and EBV(+) DLBCL frequently have a non‐functional IgH gene owing to crippling mutation. Blackwell Publishing Ltd 2000-12 /pmc/articles/PMC5926305/ /pubmed/11123421 http://dx.doi.org/10.1111/j.1349-7006.2000.tb00909.x Text en
spellingShingle Article
Kuze, Tetuo
Nakamura, Naoya
Hashimoto, Yuko
Sasaki, Yoshikazu
Abe, Masafumi
The Characteristics of Epstein‐Barr Virus (EBV)‐positive Diffuse Large B‐Cell Lymphoma: Comparison between EBV(+) and EBV(‐) Cases in Japanese Population
title The Characteristics of Epstein‐Barr Virus (EBV)‐positive Diffuse Large B‐Cell Lymphoma: Comparison between EBV(+) and EBV(‐) Cases in Japanese Population
title_full The Characteristics of Epstein‐Barr Virus (EBV)‐positive Diffuse Large B‐Cell Lymphoma: Comparison between EBV(+) and EBV(‐) Cases in Japanese Population
title_fullStr The Characteristics of Epstein‐Barr Virus (EBV)‐positive Diffuse Large B‐Cell Lymphoma: Comparison between EBV(+) and EBV(‐) Cases in Japanese Population
title_full_unstemmed The Characteristics of Epstein‐Barr Virus (EBV)‐positive Diffuse Large B‐Cell Lymphoma: Comparison between EBV(+) and EBV(‐) Cases in Japanese Population
title_short The Characteristics of Epstein‐Barr Virus (EBV)‐positive Diffuse Large B‐Cell Lymphoma: Comparison between EBV(+) and EBV(‐) Cases in Japanese Population
title_sort characteristics of epstein‐barr virus (ebv)‐positive diffuse large b‐cell lymphoma: comparison between ebv(+) and ebv(‐) cases in japanese population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926305/
https://www.ncbi.nlm.nih.gov/pubmed/11123421
http://dx.doi.org/10.1111/j.1349-7006.2000.tb00909.x
work_keys_str_mv AT kuzetetuo thecharacteristicsofepsteinbarrvirusebvpositivediffuselargebcelllymphomacomparisonbetweenebvandebvcasesinjapanesepopulation
AT nakamuranaoya thecharacteristicsofepsteinbarrvirusebvpositivediffuselargebcelllymphomacomparisonbetweenebvandebvcasesinjapanesepopulation
AT hashimotoyuko thecharacteristicsofepsteinbarrvirusebvpositivediffuselargebcelllymphomacomparisonbetweenebvandebvcasesinjapanesepopulation
AT sasakiyoshikazu thecharacteristicsofepsteinbarrvirusebvpositivediffuselargebcelllymphomacomparisonbetweenebvandebvcasesinjapanesepopulation
AT abemasafumi thecharacteristicsofepsteinbarrvirusebvpositivediffuselargebcelllymphomacomparisonbetweenebvandebvcasesinjapanesepopulation
AT kuzetetuo characteristicsofepsteinbarrvirusebvpositivediffuselargebcelllymphomacomparisonbetweenebvandebvcasesinjapanesepopulation
AT nakamuranaoya characteristicsofepsteinbarrvirusebvpositivediffuselargebcelllymphomacomparisonbetweenebvandebvcasesinjapanesepopulation
AT hashimotoyuko characteristicsofepsteinbarrvirusebvpositivediffuselargebcelllymphomacomparisonbetweenebvandebvcasesinjapanesepopulation
AT sasakiyoshikazu characteristicsofepsteinbarrvirusebvpositivediffuselargebcelllymphomacomparisonbetweenebvandebvcasesinjapanesepopulation
AT abemasafumi characteristicsofepsteinbarrvirusebvpositivediffuselargebcelllymphomacomparisonbetweenebvandebvcasesinjapanesepopulation