Cargando…

Induction of Cytotoxic T Lymphocytes from Peripheral Blood of Human Histocompatibility Antigen (HLA)‐A31(+) Gastric Cancer Patients by in vitro Stimulation with Antigenic Peptide of Signet Ring Cell Carcinoma

Antigenic peptides have been used as a cancer vaccine in melanoma patients and have led to a drastic regression of metastatic tumors. However, few antigens have been identified in non‐melanoma tumors. We recently purified a new natural antigenic peptide, designated F4.2, by biochemical elution from...

Descripción completa

Detalles Bibliográficos
Autores principales: Nabeta, Yuki, Sahara, Hiroeki, Suzuki, Kazuhiro, Kondo, Hiroaki, Nagata, Masami, Hirohashi, Yoshihiko, Sato, Yuriko, Wada, Yoshimasa, Sato, Takashi, Wada, Takuro, Yamashita, Toshihiko, Kikuchi, Kokichi, Sato, Noriyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926399/
https://www.ncbi.nlm.nih.gov/pubmed/10874214
http://dx.doi.org/10.1111/j.1349-7006.2000.tb00990.x
_version_ 1783318897952292864
author Nabeta, Yuki
Sahara, Hiroeki
Suzuki, Kazuhiro
Kondo, Hiroaki
Nagata, Masami
Hirohashi, Yoshihiko
Sato, Yuriko
Wada, Yoshimasa
Sato, Takashi
Wada, Takuro
Yamashita, Toshihiko
Kikuchi, Kokichi
Sato, Noriyuki
author_facet Nabeta, Yuki
Sahara, Hiroeki
Suzuki, Kazuhiro
Kondo, Hiroaki
Nagata, Masami
Hirohashi, Yoshihiko
Sato, Yuriko
Wada, Yoshimasa
Sato, Takashi
Wada, Takuro
Yamashita, Toshihiko
Kikuchi, Kokichi
Sato, Noriyuki
author_sort Nabeta, Yuki
collection PubMed
description Antigenic peptides have been used as a cancer vaccine in melanoma patients and have led to a drastic regression of metastatic tumors. However, few antigens have been identified in non‐melanoma tumors. We recently purified a new natural antigenic peptide, designated F4.2, by biochemical elution from a human gastric signet cell carcinoma cell line and showed that it is recognized by an autologous human histocompatibility antigen (HLA)‐A31‐restricted cytotoxic T lymphocyte (CTL) clone. Here we describe in vitro induction of F4.2‐specific CTLs from peripheral blood T lymphocytes of HLA‐A31(+) gastric cancer patients. The T cells of seven HLA‐A31(+) patients with gastric cancers were stimulated in vitro by F4.2‐pulsed autologous dendritic cells which had been induced from peripheral blood of each patient by incubation in the presence of granulocyte macrophage colony‐stimulating factor (GM‐CSF) and IL‐4. We tested the cytotoxicity of the T cells against F4.2‐loaded C1R‐A *31012 by a 6‐h (51)Cr release assay after 3 stimulations with F4.2‐pulsed dendritic cells. F4.2‐specific cytotoxicity was detectable in the stimulated T cells from two of the seven HLA‐A31(+) patients. Further, both F4.2‐specific CTLs also lysed the gastric cancer cell line, HST‐2, from which F4.2 was derived. These results suggest that F4.2 peptide may be useful as an HLA‐A31‐restricted peptide vaccine in certain patients with gastric cancer.
format Online
Article
Text
id pubmed-5926399
institution National Center for Biotechnology Information
language English
publishDate 2000
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59263992018-05-11 Induction of Cytotoxic T Lymphocytes from Peripheral Blood of Human Histocompatibility Antigen (HLA)‐A31(+) Gastric Cancer Patients by in vitro Stimulation with Antigenic Peptide of Signet Ring Cell Carcinoma Nabeta, Yuki Sahara, Hiroeki Suzuki, Kazuhiro Kondo, Hiroaki Nagata, Masami Hirohashi, Yoshihiko Sato, Yuriko Wada, Yoshimasa Sato, Takashi Wada, Takuro Yamashita, Toshihiko Kikuchi, Kokichi Sato, Noriyuki Jpn J Cancer Res Article Antigenic peptides have been used as a cancer vaccine in melanoma patients and have led to a drastic regression of metastatic tumors. However, few antigens have been identified in non‐melanoma tumors. We recently purified a new natural antigenic peptide, designated F4.2, by biochemical elution from a human gastric signet cell carcinoma cell line and showed that it is recognized by an autologous human histocompatibility antigen (HLA)‐A31‐restricted cytotoxic T lymphocyte (CTL) clone. Here we describe in vitro induction of F4.2‐specific CTLs from peripheral blood T lymphocytes of HLA‐A31(+) gastric cancer patients. The T cells of seven HLA‐A31(+) patients with gastric cancers were stimulated in vitro by F4.2‐pulsed autologous dendritic cells which had been induced from peripheral blood of each patient by incubation in the presence of granulocyte macrophage colony‐stimulating factor (GM‐CSF) and IL‐4. We tested the cytotoxicity of the T cells against F4.2‐loaded C1R‐A *31012 by a 6‐h (51)Cr release assay after 3 stimulations with F4.2‐pulsed dendritic cells. F4.2‐specific cytotoxicity was detectable in the stimulated T cells from two of the seven HLA‐A31(+) patients. Further, both F4.2‐specific CTLs also lysed the gastric cancer cell line, HST‐2, from which F4.2 was derived. These results suggest that F4.2 peptide may be useful as an HLA‐A31‐restricted peptide vaccine in certain patients with gastric cancer. Blackwell Publishing Ltd 2000-06 /pmc/articles/PMC5926399/ /pubmed/10874214 http://dx.doi.org/10.1111/j.1349-7006.2000.tb00990.x Text en
spellingShingle Article
Nabeta, Yuki
Sahara, Hiroeki
Suzuki, Kazuhiro
Kondo, Hiroaki
Nagata, Masami
Hirohashi, Yoshihiko
Sato, Yuriko
Wada, Yoshimasa
Sato, Takashi
Wada, Takuro
Yamashita, Toshihiko
Kikuchi, Kokichi
Sato, Noriyuki
Induction of Cytotoxic T Lymphocytes from Peripheral Blood of Human Histocompatibility Antigen (HLA)‐A31(+) Gastric Cancer Patients by in vitro Stimulation with Antigenic Peptide of Signet Ring Cell Carcinoma
title Induction of Cytotoxic T Lymphocytes from Peripheral Blood of Human Histocompatibility Antigen (HLA)‐A31(+) Gastric Cancer Patients by in vitro Stimulation with Antigenic Peptide of Signet Ring Cell Carcinoma
title_full Induction of Cytotoxic T Lymphocytes from Peripheral Blood of Human Histocompatibility Antigen (HLA)‐A31(+) Gastric Cancer Patients by in vitro Stimulation with Antigenic Peptide of Signet Ring Cell Carcinoma
title_fullStr Induction of Cytotoxic T Lymphocytes from Peripheral Blood of Human Histocompatibility Antigen (HLA)‐A31(+) Gastric Cancer Patients by in vitro Stimulation with Antigenic Peptide of Signet Ring Cell Carcinoma
title_full_unstemmed Induction of Cytotoxic T Lymphocytes from Peripheral Blood of Human Histocompatibility Antigen (HLA)‐A31(+) Gastric Cancer Patients by in vitro Stimulation with Antigenic Peptide of Signet Ring Cell Carcinoma
title_short Induction of Cytotoxic T Lymphocytes from Peripheral Blood of Human Histocompatibility Antigen (HLA)‐A31(+) Gastric Cancer Patients by in vitro Stimulation with Antigenic Peptide of Signet Ring Cell Carcinoma
title_sort induction of cytotoxic t lymphocytes from peripheral blood of human histocompatibility antigen (hla)‐a31(+) gastric cancer patients by in vitro stimulation with antigenic peptide of signet ring cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926399/
https://www.ncbi.nlm.nih.gov/pubmed/10874214
http://dx.doi.org/10.1111/j.1349-7006.2000.tb00990.x
work_keys_str_mv AT nabetayuki inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT saharahiroeki inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT suzukikazuhiro inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT kondohiroaki inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT nagatamasami inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT hirohashiyoshihiko inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT satoyuriko inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT wadayoshimasa inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT satotakashi inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT wadatakuro inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT yamashitatoshihiko inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT kikuchikokichi inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma
AT satonoriyuki inductionofcytotoxictlymphocytesfromperipheralbloodofhumanhistocompatibilityantigenhlaa31gastriccancerpatientsbyinvitrostimulationwithantigenicpeptideofsignetringcellcarcinoma