Cargando…
Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits
The mechanism of weight loss induced by the growth of malignant tumors is still unknown. We investigated it by focusing on apoptosis of skeletal muscle. VX2‐tumor was implanted into rabbits and the apoptotic index (AI) of skeletal muscle was measured by in situ end‐labeling assay. Plasma of the tumo...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2001
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926587/ https://www.ncbi.nlm.nih.gov/pubmed/11173541 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01044.x |
_version_ | 1783318936241045504 |
---|---|
author | Ishiko, Osamu Sumi, Toshiyuki Hirai, Kouzo Honda, Ken‐ichi Nakata, Shinichi Yoshida, Hiroyuki Ogita, Sachio |
author_facet | Ishiko, Osamu Sumi, Toshiyuki Hirai, Kouzo Honda, Ken‐ichi Nakata, Shinichi Yoshida, Hiroyuki Ogita, Sachio |
author_sort | Ishiko, Osamu |
collection | PubMed |
description | The mechanism of weight loss induced by the growth of malignant tumors is still unknown. We investigated it by focusing on apoptosis of skeletal muscle. VX2‐tumor was implanted into rabbits and the apoptotic index (AI) of skeletal muscle was measured by in situ end‐labeling assay. Plasma of the tumor‐bearing rabbits was perfused repeatedly through non‐coated charcoal resin. The AI reached 54.6% early after tumor implantation, when weight loss amounted to an 18% decrease in lean body mass (LBM) without change in muscle DNA synthesis or urinary 3‐methylhistidine/ creatinine ratio (3‐MH/Cr). When the decrease of LBM reached 30%, DNA synthesis was decreased by 48% and 3‐MH/Cr was increased by 104%, whereas AI was only 4.7%. The plasma perfusion did not prevent apoptosis in muscle, but unproved LBM, DNA synthesis, and 3‐MH/Cr. There may be two mechanisms of muscle depletion during the tumor growth: apoptosis in the early stage and metabolic abnormalities in muscle in the late stage. |
format | Online Article Text |
id | pubmed-5926587 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59265872018-05-11 Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits Ishiko, Osamu Sumi, Toshiyuki Hirai, Kouzo Honda, Ken‐ichi Nakata, Shinichi Yoshida, Hiroyuki Ogita, Sachio Jpn J Cancer Res Article The mechanism of weight loss induced by the growth of malignant tumors is still unknown. We investigated it by focusing on apoptosis of skeletal muscle. VX2‐tumor was implanted into rabbits and the apoptotic index (AI) of skeletal muscle was measured by in situ end‐labeling assay. Plasma of the tumor‐bearing rabbits was perfused repeatedly through non‐coated charcoal resin. The AI reached 54.6% early after tumor implantation, when weight loss amounted to an 18% decrease in lean body mass (LBM) without change in muscle DNA synthesis or urinary 3‐methylhistidine/ creatinine ratio (3‐MH/Cr). When the decrease of LBM reached 30%, DNA synthesis was decreased by 48% and 3‐MH/Cr was increased by 104%, whereas AI was only 4.7%. The plasma perfusion did not prevent apoptosis in muscle, but unproved LBM, DNA synthesis, and 3‐MH/Cr. There may be two mechanisms of muscle depletion during the tumor growth: apoptosis in the early stage and metabolic abnormalities in muscle in the late stage. Blackwell Publishing Ltd 2001-01 /pmc/articles/PMC5926587/ /pubmed/11173541 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01044.x Text en |
spellingShingle | Article Ishiko, Osamu Sumi, Toshiyuki Hirai, Kouzo Honda, Ken‐ichi Nakata, Shinichi Yoshida, Hiroyuki Ogita, Sachio Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits |
title | Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits |
title_full | Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits |
title_fullStr | Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits |
title_full_unstemmed | Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits |
title_short | Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits |
title_sort | apoptosis of muscle cells causes weight loss prior to impairment of dna synthesis in tumor‐bearing rabbits |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926587/ https://www.ncbi.nlm.nih.gov/pubmed/11173541 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01044.x |
work_keys_str_mv | AT ishikoosamu apoptosisofmusclecellscausesweightlosspriortoimpairmentofdnasynthesisintumorbearingrabbits AT sumitoshiyuki apoptosisofmusclecellscausesweightlosspriortoimpairmentofdnasynthesisintumorbearingrabbits AT hiraikouzo apoptosisofmusclecellscausesweightlosspriortoimpairmentofdnasynthesisintumorbearingrabbits AT hondakenichi apoptosisofmusclecellscausesweightlosspriortoimpairmentofdnasynthesisintumorbearingrabbits AT nakatashinichi apoptosisofmusclecellscausesweightlosspriortoimpairmentofdnasynthesisintumorbearingrabbits AT yoshidahiroyuki apoptosisofmusclecellscausesweightlosspriortoimpairmentofdnasynthesisintumorbearingrabbits AT ogitasachio apoptosisofmusclecellscausesweightlosspriortoimpairmentofdnasynthesisintumorbearingrabbits |