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Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits

The mechanism of weight loss induced by the growth of malignant tumors is still unknown. We investigated it by focusing on apoptosis of skeletal muscle. VX2‐tumor was implanted into rabbits and the apoptotic index (AI) of skeletal muscle was measured by in situ end‐labeling assay. Plasma of the tumo...

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Detalles Bibliográficos
Autores principales: Ishiko, Osamu, Sumi, Toshiyuki, Hirai, Kouzo, Honda, Ken‐ichi, Nakata, Shinichi, Yoshida, Hiroyuki, Ogita, Sachio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926587/
https://www.ncbi.nlm.nih.gov/pubmed/11173541
http://dx.doi.org/10.1111/j.1349-7006.2001.tb01044.x
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author Ishiko, Osamu
Sumi, Toshiyuki
Hirai, Kouzo
Honda, Ken‐ichi
Nakata, Shinichi
Yoshida, Hiroyuki
Ogita, Sachio
author_facet Ishiko, Osamu
Sumi, Toshiyuki
Hirai, Kouzo
Honda, Ken‐ichi
Nakata, Shinichi
Yoshida, Hiroyuki
Ogita, Sachio
author_sort Ishiko, Osamu
collection PubMed
description The mechanism of weight loss induced by the growth of malignant tumors is still unknown. We investigated it by focusing on apoptosis of skeletal muscle. VX2‐tumor was implanted into rabbits and the apoptotic index (AI) of skeletal muscle was measured by in situ end‐labeling assay. Plasma of the tumor‐bearing rabbits was perfused repeatedly through non‐coated charcoal resin. The AI reached 54.6% early after tumor implantation, when weight loss amounted to an 18% decrease in lean body mass (LBM) without change in muscle DNA synthesis or urinary 3‐methylhistidine/ creatinine ratio (3‐MH/Cr). When the decrease of LBM reached 30%, DNA synthesis was decreased by 48% and 3‐MH/Cr was increased by 104%, whereas AI was only 4.7%. The plasma perfusion did not prevent apoptosis in muscle, but unproved LBM, DNA synthesis, and 3‐MH/Cr. There may be two mechanisms of muscle depletion during the tumor growth: apoptosis in the early stage and metabolic abnormalities in muscle in the late stage.
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spelling pubmed-59265872018-05-11 Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits Ishiko, Osamu Sumi, Toshiyuki Hirai, Kouzo Honda, Ken‐ichi Nakata, Shinichi Yoshida, Hiroyuki Ogita, Sachio Jpn J Cancer Res Article The mechanism of weight loss induced by the growth of malignant tumors is still unknown. We investigated it by focusing on apoptosis of skeletal muscle. VX2‐tumor was implanted into rabbits and the apoptotic index (AI) of skeletal muscle was measured by in situ end‐labeling assay. Plasma of the tumor‐bearing rabbits was perfused repeatedly through non‐coated charcoal resin. The AI reached 54.6% early after tumor implantation, when weight loss amounted to an 18% decrease in lean body mass (LBM) without change in muscle DNA synthesis or urinary 3‐methylhistidine/ creatinine ratio (3‐MH/Cr). When the decrease of LBM reached 30%, DNA synthesis was decreased by 48% and 3‐MH/Cr was increased by 104%, whereas AI was only 4.7%. The plasma perfusion did not prevent apoptosis in muscle, but unproved LBM, DNA synthesis, and 3‐MH/Cr. There may be two mechanisms of muscle depletion during the tumor growth: apoptosis in the early stage and metabolic abnormalities in muscle in the late stage. Blackwell Publishing Ltd 2001-01 /pmc/articles/PMC5926587/ /pubmed/11173541 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01044.x Text en
spellingShingle Article
Ishiko, Osamu
Sumi, Toshiyuki
Hirai, Kouzo
Honda, Ken‐ichi
Nakata, Shinichi
Yoshida, Hiroyuki
Ogita, Sachio
Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits
title Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits
title_full Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits
title_fullStr Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits
title_full_unstemmed Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits
title_short Apoptosis of Muscle Cells Causes Weight Loss Prior to Impairment of DNA Synthesis in Tumor‐bearing Rabbits
title_sort apoptosis of muscle cells causes weight loss prior to impairment of dna synthesis in tumor‐bearing rabbits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926587/
https://www.ncbi.nlm.nih.gov/pubmed/11173541
http://dx.doi.org/10.1111/j.1349-7006.2001.tb01044.x
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