Aberrations of the p14(ARF) and p16(INK4a) Genes in Renal Cell Carcinomas

The INK4alARF locus on chromosome 9p21, which encodes two distinct genes, p14(ARF) and p16(INK4a), is frequently altered in human neoplasms. To investigate the potential roles of p14(ARF) and p16(IfiK4a) genes in human renal cell carcinomas (RCCs), we analyzed 6 human RCC cell lines and 91 primary R...

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Detalles Bibliográficos
Autores principales: Kawada, Yoichi, Nakamura, Mitsutoshi, Ishida, Eiwa, Shimada, Keiji, Oosterwijk, Egbert, Uemura, Hirotsugu, Hirao, Yoshihiko, Chul, Kim Sung, Konishi, Noboru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2001
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926680/
https://www.ncbi.nlm.nih.gov/pubmed/11749694
http://dx.doi.org/10.1111/j.1349-7006.2001.tb02152.x
Descripción
Sumario:The INK4alARF locus on chromosome 9p21, which encodes two distinct genes, p14(ARF) and p16(INK4a), is frequently altered in human neoplasms. To investigate the potential roles of p14(ARF) and p16(IfiK4a) genes in human renal cell carcinomas (RCCs), we analyzed 6 human RCC cell lines and 91 primary RCCs for homozygous deletion, promoter hypermethylation and expression of the p14(ARF) and p16(INK4a) gene products using differential PCR, methylation‐specific PCR, and immunohistochemis‐try, respectively. Five cell lines showed homozygous co‐deletion of both genes and one demonstrated promoter hypermethylation of the p16(INK4a) gene only. Eight of 91 RCCs showed aberrations of p14(ARF) or p16(INK4a) status and six of these featured gross extension into the renal vein. The results suggest that p14(ARF) and p16(INK4a) aberrations may play roles in the relatively late stage of renal tumorigenesis associated with tumor progression.

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