Aberrations of the p14(ARF) and p16(INK4a) Genes in Renal Cell Carcinomas

The INK4alARF locus on chromosome 9p21, which encodes two distinct genes, p14(ARF) and p16(INK4a), is frequently altered in human neoplasms. To investigate the potential roles of p14(ARF) and p16(IfiK4a) genes in human renal cell carcinomas (RCCs), we analyzed 6 human RCC cell lines and 91 primary R...

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Autores principales: Kawada, Yoichi, Nakamura, Mitsutoshi, Ishida, Eiwa, Shimada, Keiji, Oosterwijk, Egbert, Uemura, Hirotsugu, Hirao, Yoshihiko, Chul, Kim Sung, Konishi, Noboru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2001
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926680/
https://www.ncbi.nlm.nih.gov/pubmed/11749694
http://dx.doi.org/10.1111/j.1349-7006.2001.tb02152.x
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author Kawada, Yoichi
Nakamura, Mitsutoshi
Ishida, Eiwa
Shimada, Keiji
Oosterwijk, Egbert
Uemura, Hirotsugu
Hirao, Yoshihiko
Chul, Kim Sung
Konishi, Noboru
author_facet Kawada, Yoichi
Nakamura, Mitsutoshi
Ishida, Eiwa
Shimada, Keiji
Oosterwijk, Egbert
Uemura, Hirotsugu
Hirao, Yoshihiko
Chul, Kim Sung
Konishi, Noboru
author_sort Kawada, Yoichi
collection PubMed
description The INK4alARF locus on chromosome 9p21, which encodes two distinct genes, p14(ARF) and p16(INK4a), is frequently altered in human neoplasms. To investigate the potential roles of p14(ARF) and p16(IfiK4a) genes in human renal cell carcinomas (RCCs), we analyzed 6 human RCC cell lines and 91 primary RCCs for homozygous deletion, promoter hypermethylation and expression of the p14(ARF) and p16(INK4a) gene products using differential PCR, methylation‐specific PCR, and immunohistochemis‐try, respectively. Five cell lines showed homozygous co‐deletion of both genes and one demonstrated promoter hypermethylation of the p16(INK4a) gene only. Eight of 91 RCCs showed aberrations of p14(ARF) or p16(INK4a) status and six of these featured gross extension into the renal vein. The results suggest that p14(ARF) and p16(INK4a) aberrations may play roles in the relatively late stage of renal tumorigenesis associated with tumor progression.
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spelling pubmed-59266802018-05-11 Aberrations of the p14(ARF) and p16(INK4a) Genes in Renal Cell Carcinomas Kawada, Yoichi Nakamura, Mitsutoshi Ishida, Eiwa Shimada, Keiji Oosterwijk, Egbert Uemura, Hirotsugu Hirao, Yoshihiko Chul, Kim Sung Konishi, Noboru Jpn J Cancer Res Article The INK4alARF locus on chromosome 9p21, which encodes two distinct genes, p14(ARF) and p16(INK4a), is frequently altered in human neoplasms. To investigate the potential roles of p14(ARF) and p16(IfiK4a) genes in human renal cell carcinomas (RCCs), we analyzed 6 human RCC cell lines and 91 primary RCCs for homozygous deletion, promoter hypermethylation and expression of the p14(ARF) and p16(INK4a) gene products using differential PCR, methylation‐specific PCR, and immunohistochemis‐try, respectively. Five cell lines showed homozygous co‐deletion of both genes and one demonstrated promoter hypermethylation of the p16(INK4a) gene only. Eight of 91 RCCs showed aberrations of p14(ARF) or p16(INK4a) status and six of these featured gross extension into the renal vein. The results suggest that p14(ARF) and p16(INK4a) aberrations may play roles in the relatively late stage of renal tumorigenesis associated with tumor progression. Blackwell Publishing Ltd 2001-12 /pmc/articles/PMC5926680/ /pubmed/11749694 http://dx.doi.org/10.1111/j.1349-7006.2001.tb02152.x Text en
spellingShingle Article
Kawada, Yoichi
Nakamura, Mitsutoshi
Ishida, Eiwa
Shimada, Keiji
Oosterwijk, Egbert
Uemura, Hirotsugu
Hirao, Yoshihiko
Chul, Kim Sung
Konishi, Noboru
Aberrations of the p14(ARF) and p16(INK4a) Genes in Renal Cell Carcinomas
title Aberrations of the p14(ARF) and p16(INK4a) Genes in Renal Cell Carcinomas
title_full Aberrations of the p14(ARF) and p16(INK4a) Genes in Renal Cell Carcinomas
title_fullStr Aberrations of the p14(ARF) and p16(INK4a) Genes in Renal Cell Carcinomas
title_full_unstemmed Aberrations of the p14(ARF) and p16(INK4a) Genes in Renal Cell Carcinomas
title_short Aberrations of the p14(ARF) and p16(INK4a) Genes in Renal Cell Carcinomas
title_sort aberrations of the p14(arf) and p16(ink4a) genes in renal cell carcinomas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926680/
https://www.ncbi.nlm.nih.gov/pubmed/11749694
http://dx.doi.org/10.1111/j.1349-7006.2001.tb02152.x
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