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Possible Involvement of Interferon Regulatory Factor 4 (IRF4) in a Clinical Subtype of Adult T‐Cell Leukemia

Interferon regulatory factor (IRF) 4 is the lymphoid‐specific transcription factor that is required for the proliferation of mitogen‐activated T cells. IRF4 has been suggested to be involved in tuniorigenesis because the overexpression of IRF4 caused the transformation of Rat‐1 fibroblasts in vitro....

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Autores principales: Imaizumi, Yoshitaka, Kohno, Tomoko, Yamada, Yasuaki, Ikeda, Shuichi, Tanaka, Yuetsu, Tomonaga, Masao, Matsuyama, Toshifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926682/
https://www.ncbi.nlm.nih.gov/pubmed/11749693
http://dx.doi.org/10.1111/j.1349-7006.2001.tb02151.x
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author Imaizumi, Yoshitaka
Kohno, Tomoko
Yamada, Yasuaki
Ikeda, Shuichi
Tanaka, Yuetsu
Tomonaga, Masao
Matsuyama, Toshifumi
author_facet Imaizumi, Yoshitaka
Kohno, Tomoko
Yamada, Yasuaki
Ikeda, Shuichi
Tanaka, Yuetsu
Tomonaga, Masao
Matsuyama, Toshifumi
author_sort Imaizumi, Yoshitaka
collection PubMed
description Interferon regulatory factor (IRF) 4 is the lymphoid‐specific transcription factor that is required for the proliferation of mitogen‐activated T cells. IRF4 has been suggested to be involved in tuniorigenesis because the overexpression of IRF4 caused the transformation of Rat‐1 fibroblasts in vitro. Here, we show that IRF4 is constitutively expressed in adult T‐cell leukemia (ATL)‐derived cell lines, which were infected with human T‐cell leukemia virus type‐I, but hardly expressed the trans‐activator protein, Tax. Similarly, constitutive expression of IRF4 was demonstrated in freshly isolated peripheral blood mononuclear cells (PBMC) from patients with either acute or chronic ATL. However, the high‐level expression of IRF4 was specifically associated with acute ATL. With mitogen‐activated PBMC from healthy donors, cell cycle analyses revealed that the induction of IRF4 occurred prior to cell cycle progression and the cells that had entered the cell cycle were predominantly IRF4‐positive cells. In addition, ectopic expression of IRF4 in Rat‐1 fibroblasts increased the S and G2/M phase population significantly. Taken together, our results indicate that IRF4 is involved in the pathogenesis of ATL through its positive effect on the cell cycle, and that IRF4 can be used as a molecular marker of clinical subtype in ATL.
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spelling pubmed-59266822018-05-11 Possible Involvement of Interferon Regulatory Factor 4 (IRF4) in a Clinical Subtype of Adult T‐Cell Leukemia Imaizumi, Yoshitaka Kohno, Tomoko Yamada, Yasuaki Ikeda, Shuichi Tanaka, Yuetsu Tomonaga, Masao Matsuyama, Toshifumi Jpn J Cancer Res Article Interferon regulatory factor (IRF) 4 is the lymphoid‐specific transcription factor that is required for the proliferation of mitogen‐activated T cells. IRF4 has been suggested to be involved in tuniorigenesis because the overexpression of IRF4 caused the transformation of Rat‐1 fibroblasts in vitro. Here, we show that IRF4 is constitutively expressed in adult T‐cell leukemia (ATL)‐derived cell lines, which were infected with human T‐cell leukemia virus type‐I, but hardly expressed the trans‐activator protein, Tax. Similarly, constitutive expression of IRF4 was demonstrated in freshly isolated peripheral blood mononuclear cells (PBMC) from patients with either acute or chronic ATL. However, the high‐level expression of IRF4 was specifically associated with acute ATL. With mitogen‐activated PBMC from healthy donors, cell cycle analyses revealed that the induction of IRF4 occurred prior to cell cycle progression and the cells that had entered the cell cycle were predominantly IRF4‐positive cells. In addition, ectopic expression of IRF4 in Rat‐1 fibroblasts increased the S and G2/M phase population significantly. Taken together, our results indicate that IRF4 is involved in the pathogenesis of ATL through its positive effect on the cell cycle, and that IRF4 can be used as a molecular marker of clinical subtype in ATL. Blackwell Publishing Ltd 2001-12 /pmc/articles/PMC5926682/ /pubmed/11749693 http://dx.doi.org/10.1111/j.1349-7006.2001.tb02151.x Text en
spellingShingle Article
Imaizumi, Yoshitaka
Kohno, Tomoko
Yamada, Yasuaki
Ikeda, Shuichi
Tanaka, Yuetsu
Tomonaga, Masao
Matsuyama, Toshifumi
Possible Involvement of Interferon Regulatory Factor 4 (IRF4) in a Clinical Subtype of Adult T‐Cell Leukemia
title Possible Involvement of Interferon Regulatory Factor 4 (IRF4) in a Clinical Subtype of Adult T‐Cell Leukemia
title_full Possible Involvement of Interferon Regulatory Factor 4 (IRF4) in a Clinical Subtype of Adult T‐Cell Leukemia
title_fullStr Possible Involvement of Interferon Regulatory Factor 4 (IRF4) in a Clinical Subtype of Adult T‐Cell Leukemia
title_full_unstemmed Possible Involvement of Interferon Regulatory Factor 4 (IRF4) in a Clinical Subtype of Adult T‐Cell Leukemia
title_short Possible Involvement of Interferon Regulatory Factor 4 (IRF4) in a Clinical Subtype of Adult T‐Cell Leukemia
title_sort possible involvement of interferon regulatory factor 4 (irf4) in a clinical subtype of adult t‐cell leukemia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926682/
https://www.ncbi.nlm.nih.gov/pubmed/11749693
http://dx.doi.org/10.1111/j.1349-7006.2001.tb02151.x
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