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Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells
Cytotoxic T lymphocytes (CTL) against human lung cancer cells are difficult to induce by a conventional method using tumor cell stimulation probably due to an insufficiency of tumor antigens (TA) or costimulatory molecules such as CD80. We, therefore, investigated the potential of CD80‐transfected t...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926714/ https://www.ncbi.nlm.nih.gov/pubmed/11267941 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01096.x |
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author | Takenoyama, Mitsuhiro Yoshino, Ichiro Eifuku, Ryozo So, Tomoko Imahayashi, Satoru Sugaya, Masakazu Yasuda, Manabu Inoue, Masaaki Ichiyoshi, Yuji Osaki, Toshihiro Nagashima, Akira Nomoto, Kikuo Yasumoto, Kosei |
author_facet | Takenoyama, Mitsuhiro Yoshino, Ichiro Eifuku, Ryozo So, Tomoko Imahayashi, Satoru Sugaya, Masakazu Yasuda, Manabu Inoue, Masaaki Ichiyoshi, Yuji Osaki, Toshihiro Nagashima, Akira Nomoto, Kikuo Yasumoto, Kosei |
author_sort | Takenoyama, Mitsuhiro |
collection | PubMed |
description | Cytotoxic T lymphocytes (CTL) against human lung cancer cells are difficult to induce by a conventional method using tumor cell stimulation probably due to an insufficiency of tumor antigens (TA) or costimulatory molecules such as CD80. We, therefore, investigated the potential of CD80‐transfected tumor cells as stimulators of the in vitro induction of autologous tumor‐specific CTL from regional lymph node lymphocytes in patients with lung cancer. Five non‐small cell lung cancer cell lines (two adenocarcinomas, 1 squamous cell carcinoma, 1 large cell carcinoma and 1 adenosquamous cell carcinoma) were established from surgical specimens and were successfully transduced with a plasmid constructed with expression vector pBj and human CD80 cDNA, using a lipofection method. CD80‐transfected tumor cells (CD80‐AT) significantly augmented the proliferation of autologous lymphocytes from all cases as compared with non‐transfected tumor cells (AT). AT‐stimulated lymphocytes from 4 out of 5 cases did not show any cytotoxicity against AT; however, lymphocytes stimulated with CD80‐AT exhibited substantial cytotoxicity against parental AT in all 5 cases tested. AT‐stimulated lymphocytes derived from only one out of 5 cases showed major histocompatibility complex (MHC)‐class I‐restricted cytokine production in response to AT, while the MHC‐class I‐restricted responses were found in CD80‐AT‐stimulated lymphocytes from 4 out of 5 cases. These results indicate that CD80 on tumor cells could be a beneficial costimulatory molecule to elicit CTL against lung cancer, and also show that TA recognized by CTL was frequently expressed on lung cancer cells. |
format | Online Article Text |
id | pubmed-5926714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59267142018-05-11 Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells Takenoyama, Mitsuhiro Yoshino, Ichiro Eifuku, Ryozo So, Tomoko Imahayashi, Satoru Sugaya, Masakazu Yasuda, Manabu Inoue, Masaaki Ichiyoshi, Yuji Osaki, Toshihiro Nagashima, Akira Nomoto, Kikuo Yasumoto, Kosei Jpn J Cancer Res Article Cytotoxic T lymphocytes (CTL) against human lung cancer cells are difficult to induce by a conventional method using tumor cell stimulation probably due to an insufficiency of tumor antigens (TA) or costimulatory molecules such as CD80. We, therefore, investigated the potential of CD80‐transfected tumor cells as stimulators of the in vitro induction of autologous tumor‐specific CTL from regional lymph node lymphocytes in patients with lung cancer. Five non‐small cell lung cancer cell lines (two adenocarcinomas, 1 squamous cell carcinoma, 1 large cell carcinoma and 1 adenosquamous cell carcinoma) were established from surgical specimens and were successfully transduced with a plasmid constructed with expression vector pBj and human CD80 cDNA, using a lipofection method. CD80‐transfected tumor cells (CD80‐AT) significantly augmented the proliferation of autologous lymphocytes from all cases as compared with non‐transfected tumor cells (AT). AT‐stimulated lymphocytes from 4 out of 5 cases did not show any cytotoxicity against AT; however, lymphocytes stimulated with CD80‐AT exhibited substantial cytotoxicity against parental AT in all 5 cases tested. AT‐stimulated lymphocytes derived from only one out of 5 cases showed major histocompatibility complex (MHC)‐class I‐restricted cytokine production in response to AT, while the MHC‐class I‐restricted responses were found in CD80‐AT‐stimulated lymphocytes from 4 out of 5 cases. These results indicate that CD80 on tumor cells could be a beneficial costimulatory molecule to elicit CTL against lung cancer, and also show that TA recognized by CTL was frequently expressed on lung cancer cells. Blackwell Publishing Ltd 2001-03 /pmc/articles/PMC5926714/ /pubmed/11267941 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01096.x Text en |
spellingShingle | Article Takenoyama, Mitsuhiro Yoshino, Ichiro Eifuku, Ryozo So, Tomoko Imahayashi, Satoru Sugaya, Masakazu Yasuda, Manabu Inoue, Masaaki Ichiyoshi, Yuji Osaki, Toshihiro Nagashima, Akira Nomoto, Kikuo Yasumoto, Kosei Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells |
title | Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells |
title_full | Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells |
title_fullStr | Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells |
title_full_unstemmed | Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells |
title_short | Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells |
title_sort | successful induction of tumor‐specific cytotoxic t lymphocytes from patients with non‐small cell lung cancer using cd80‐transfected autologous tumor cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926714/ https://www.ncbi.nlm.nih.gov/pubmed/11267941 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01096.x |
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