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Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells

Cytotoxic T lymphocytes (CTL) against human lung cancer cells are difficult to induce by a conventional method using tumor cell stimulation probably due to an insufficiency of tumor antigens (TA) or costimulatory molecules such as CD80. We, therefore, investigated the potential of CD80‐transfected t...

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Autores principales: Takenoyama, Mitsuhiro, Yoshino, Ichiro, Eifuku, Ryozo, So, Tomoko, Imahayashi, Satoru, Sugaya, Masakazu, Yasuda, Manabu, Inoue, Masaaki, Ichiyoshi, Yuji, Osaki, Toshihiro, Nagashima, Akira, Nomoto, Kikuo, Yasumoto, Kosei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926714/
https://www.ncbi.nlm.nih.gov/pubmed/11267941
http://dx.doi.org/10.1111/j.1349-7006.2001.tb01096.x
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author Takenoyama, Mitsuhiro
Yoshino, Ichiro
Eifuku, Ryozo
So, Tomoko
Imahayashi, Satoru
Sugaya, Masakazu
Yasuda, Manabu
Inoue, Masaaki
Ichiyoshi, Yuji
Osaki, Toshihiro
Nagashima, Akira
Nomoto, Kikuo
Yasumoto, Kosei
author_facet Takenoyama, Mitsuhiro
Yoshino, Ichiro
Eifuku, Ryozo
So, Tomoko
Imahayashi, Satoru
Sugaya, Masakazu
Yasuda, Manabu
Inoue, Masaaki
Ichiyoshi, Yuji
Osaki, Toshihiro
Nagashima, Akira
Nomoto, Kikuo
Yasumoto, Kosei
author_sort Takenoyama, Mitsuhiro
collection PubMed
description Cytotoxic T lymphocytes (CTL) against human lung cancer cells are difficult to induce by a conventional method using tumor cell stimulation probably due to an insufficiency of tumor antigens (TA) or costimulatory molecules such as CD80. We, therefore, investigated the potential of CD80‐transfected tumor cells as stimulators of the in vitro induction of autologous tumor‐specific CTL from regional lymph node lymphocytes in patients with lung cancer. Five non‐small cell lung cancer cell lines (two adenocarcinomas, 1 squamous cell carcinoma, 1 large cell carcinoma and 1 adenosquamous cell carcinoma) were established from surgical specimens and were successfully transduced with a plasmid constructed with expression vector pBj and human CD80 cDNA, using a lipofection method. CD80‐transfected tumor cells (CD80‐AT) significantly augmented the proliferation of autologous lymphocytes from all cases as compared with non‐transfected tumor cells (AT). AT‐stimulated lymphocytes from 4 out of 5 cases did not show any cytotoxicity against AT; however, lymphocytes stimulated with CD80‐AT exhibited substantial cytotoxicity against parental AT in all 5 cases tested. AT‐stimulated lymphocytes derived from only one out of 5 cases showed major histocompatibility complex (MHC)‐class I‐restricted cytokine production in response to AT, while the MHC‐class I‐restricted responses were found in CD80‐AT‐stimulated lymphocytes from 4 out of 5 cases. These results indicate that CD80 on tumor cells could be a beneficial costimulatory molecule to elicit CTL against lung cancer, and also show that TA recognized by CTL was frequently expressed on lung cancer cells.
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spelling pubmed-59267142018-05-11 Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells Takenoyama, Mitsuhiro Yoshino, Ichiro Eifuku, Ryozo So, Tomoko Imahayashi, Satoru Sugaya, Masakazu Yasuda, Manabu Inoue, Masaaki Ichiyoshi, Yuji Osaki, Toshihiro Nagashima, Akira Nomoto, Kikuo Yasumoto, Kosei Jpn J Cancer Res Article Cytotoxic T lymphocytes (CTL) against human lung cancer cells are difficult to induce by a conventional method using tumor cell stimulation probably due to an insufficiency of tumor antigens (TA) or costimulatory molecules such as CD80. We, therefore, investigated the potential of CD80‐transfected tumor cells as stimulators of the in vitro induction of autologous tumor‐specific CTL from regional lymph node lymphocytes in patients with lung cancer. Five non‐small cell lung cancer cell lines (two adenocarcinomas, 1 squamous cell carcinoma, 1 large cell carcinoma and 1 adenosquamous cell carcinoma) were established from surgical specimens and were successfully transduced with a plasmid constructed with expression vector pBj and human CD80 cDNA, using a lipofection method. CD80‐transfected tumor cells (CD80‐AT) significantly augmented the proliferation of autologous lymphocytes from all cases as compared with non‐transfected tumor cells (AT). AT‐stimulated lymphocytes from 4 out of 5 cases did not show any cytotoxicity against AT; however, lymphocytes stimulated with CD80‐AT exhibited substantial cytotoxicity against parental AT in all 5 cases tested. AT‐stimulated lymphocytes derived from only one out of 5 cases showed major histocompatibility complex (MHC)‐class I‐restricted cytokine production in response to AT, while the MHC‐class I‐restricted responses were found in CD80‐AT‐stimulated lymphocytes from 4 out of 5 cases. These results indicate that CD80 on tumor cells could be a beneficial costimulatory molecule to elicit CTL against lung cancer, and also show that TA recognized by CTL was frequently expressed on lung cancer cells. Blackwell Publishing Ltd 2001-03 /pmc/articles/PMC5926714/ /pubmed/11267941 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01096.x Text en
spellingShingle Article
Takenoyama, Mitsuhiro
Yoshino, Ichiro
Eifuku, Ryozo
So, Tomoko
Imahayashi, Satoru
Sugaya, Masakazu
Yasuda, Manabu
Inoue, Masaaki
Ichiyoshi, Yuji
Osaki, Toshihiro
Nagashima, Akira
Nomoto, Kikuo
Yasumoto, Kosei
Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells
title Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells
title_full Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells
title_fullStr Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells
title_full_unstemmed Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells
title_short Successful Induction of Tumor‐specific Cytotoxic T Lymphocytes from Patients with Non‐small Cell Lung Cancer Using CD80‐transfected Autologous Tumor Cells
title_sort successful induction of tumor‐specific cytotoxic t lymphocytes from patients with non‐small cell lung cancer using cd80‐transfected autologous tumor cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926714/
https://www.ncbi.nlm.nih.gov/pubmed/11267941
http://dx.doi.org/10.1111/j.1349-7006.2001.tb01096.x
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