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Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate
Highly unsaturated fatty acid‐containing phospholipid (HUFA‐PL) has many nutritional and medical applications. We investigated the effect of HUFA‐PL on differentiation of human leukemia HL‐60 cells induced by dibutyryl cyclic adenosine monophosphate (dbcAMP). HUFA‐containing phosphatidylethanolamine...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926757/ https://www.ncbi.nlm.nih.gov/pubmed/11429056 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01146.x |
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author | Hosokawa, Masashi Sato, Akiyuki Ishigamori, Hideki Kohno, Hiroyuki Tanaka, Takuji Takahashi, Koretaro |
author_facet | Hosokawa, Masashi Sato, Akiyuki Ishigamori, Hideki Kohno, Hiroyuki Tanaka, Takuji Takahashi, Koretaro |
author_sort | Hosokawa, Masashi |
collection | PubMed |
description | Highly unsaturated fatty acid‐containing phospholipid (HUFA‐PL) has many nutritional and medical applications. We investigated the effect of HUFA‐PL on differentiation of human leukemia HL‐60 cells induced by dibutyryl cyclic adenosine monophosphate (dbcAMP). HUFA‐containing phosphatidylethanolamine (HUFA‐PE), such as salmon testis PE, significantly enhanced dbcAMP ‐induced cell differentiation. A combined treatment of 200 μM dbcAMP with 50 μM HUFA‐PE increased the nitroblue tetrazolium (NBT)‐reducing activity, which is an indicator of differentiation, to a level comparable to that in the case of 500 μM dbcAMP treatment. In contrast, HUFA‐lyso PE (a monoacyl form) did not exert an enhancing effect on dbcAMP‐induced differentiation. The enhancing effect of HUFA‐PE was suppressed by a protein kinase C inhibitor, staurosporine, while a protein kinase A inhibitor, H‐8, did not suppress the enhancing effect. These findings suggest that HUFA‐PE might enhance dbcAMP‐induced differentiation through modulation of the protein kinase C signaling pathway in HL‐60 cells. |
format | Online Article Text |
id | pubmed-5926757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59267572018-05-11 Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate Hosokawa, Masashi Sato, Akiyuki Ishigamori, Hideki Kohno, Hiroyuki Tanaka, Takuji Takahashi, Koretaro Jpn J Cancer Res Article Highly unsaturated fatty acid‐containing phospholipid (HUFA‐PL) has many nutritional and medical applications. We investigated the effect of HUFA‐PL on differentiation of human leukemia HL‐60 cells induced by dibutyryl cyclic adenosine monophosphate (dbcAMP). HUFA‐containing phosphatidylethanolamine (HUFA‐PE), such as salmon testis PE, significantly enhanced dbcAMP ‐induced cell differentiation. A combined treatment of 200 μM dbcAMP with 50 μM HUFA‐PE increased the nitroblue tetrazolium (NBT)‐reducing activity, which is an indicator of differentiation, to a level comparable to that in the case of 500 μM dbcAMP treatment. In contrast, HUFA‐lyso PE (a monoacyl form) did not exert an enhancing effect on dbcAMP‐induced differentiation. The enhancing effect of HUFA‐PE was suppressed by a protein kinase C inhibitor, staurosporine, while a protein kinase A inhibitor, H‐8, did not suppress the enhancing effect. These findings suggest that HUFA‐PE might enhance dbcAMP‐induced differentiation through modulation of the protein kinase C signaling pathway in HL‐60 cells. Blackwell Publishing Ltd 2001-06 /pmc/articles/PMC5926757/ /pubmed/11429056 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01146.x Text en |
spellingShingle | Article Hosokawa, Masashi Sato, Akiyuki Ishigamori, Hideki Kohno, Hiroyuki Tanaka, Takuji Takahashi, Koretaro Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate |
title | Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate |
title_full | Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate |
title_fullStr | Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate |
title_full_unstemmed | Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate |
title_short | Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate |
title_sort | synergistic effects of highly unsaturated fatty acid‐containing phosphatidyl‐ethanolamine on differentiation of human leukemia hl‐60 cells by dibutyryl cyclic adenosine monophosphate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926757/ https://www.ncbi.nlm.nih.gov/pubmed/11429056 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01146.x |
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