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Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate

Highly unsaturated fatty acid‐containing phospholipid (HUFA‐PL) has many nutritional and medical applications. We investigated the effect of HUFA‐PL on differentiation of human leukemia HL‐60 cells induced by dibutyryl cyclic adenosine monophosphate (dbcAMP). HUFA‐containing phosphatidylethanolamine...

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Detalles Bibliográficos
Autores principales: Hosokawa, Masashi, Sato, Akiyuki, Ishigamori, Hideki, Kohno, Hiroyuki, Tanaka, Takuji, Takahashi, Koretaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926757/
https://www.ncbi.nlm.nih.gov/pubmed/11429056
http://dx.doi.org/10.1111/j.1349-7006.2001.tb01146.x
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author Hosokawa, Masashi
Sato, Akiyuki
Ishigamori, Hideki
Kohno, Hiroyuki
Tanaka, Takuji
Takahashi, Koretaro
author_facet Hosokawa, Masashi
Sato, Akiyuki
Ishigamori, Hideki
Kohno, Hiroyuki
Tanaka, Takuji
Takahashi, Koretaro
author_sort Hosokawa, Masashi
collection PubMed
description Highly unsaturated fatty acid‐containing phospholipid (HUFA‐PL) has many nutritional and medical applications. We investigated the effect of HUFA‐PL on differentiation of human leukemia HL‐60 cells induced by dibutyryl cyclic adenosine monophosphate (dbcAMP). HUFA‐containing phosphatidylethanolamine (HUFA‐PE), such as salmon testis PE, significantly enhanced dbcAMP ‐induced cell differentiation. A combined treatment of 200 μM dbcAMP with 50 μM HUFA‐PE increased the nitroblue tetrazolium (NBT)‐reducing activity, which is an indicator of differentiation, to a level comparable to that in the case of 500 μM dbcAMP treatment. In contrast, HUFA‐lyso PE (a monoacyl form) did not exert an enhancing effect on dbcAMP‐induced differentiation. The enhancing effect of HUFA‐PE was suppressed by a protein kinase C inhibitor, staurosporine, while a protein kinase A inhibitor, H‐8, did not suppress the enhancing effect. These findings suggest that HUFA‐PE might enhance dbcAMP‐induced differentiation through modulation of the protein kinase C signaling pathway in HL‐60 cells.
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spelling pubmed-59267572018-05-11 Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate Hosokawa, Masashi Sato, Akiyuki Ishigamori, Hideki Kohno, Hiroyuki Tanaka, Takuji Takahashi, Koretaro Jpn J Cancer Res Article Highly unsaturated fatty acid‐containing phospholipid (HUFA‐PL) has many nutritional and medical applications. We investigated the effect of HUFA‐PL on differentiation of human leukemia HL‐60 cells induced by dibutyryl cyclic adenosine monophosphate (dbcAMP). HUFA‐containing phosphatidylethanolamine (HUFA‐PE), such as salmon testis PE, significantly enhanced dbcAMP ‐induced cell differentiation. A combined treatment of 200 μM dbcAMP with 50 μM HUFA‐PE increased the nitroblue tetrazolium (NBT)‐reducing activity, which is an indicator of differentiation, to a level comparable to that in the case of 500 μM dbcAMP treatment. In contrast, HUFA‐lyso PE (a monoacyl form) did not exert an enhancing effect on dbcAMP‐induced differentiation. The enhancing effect of HUFA‐PE was suppressed by a protein kinase C inhibitor, staurosporine, while a protein kinase A inhibitor, H‐8, did not suppress the enhancing effect. These findings suggest that HUFA‐PE might enhance dbcAMP‐induced differentiation through modulation of the protein kinase C signaling pathway in HL‐60 cells. Blackwell Publishing Ltd 2001-06 /pmc/articles/PMC5926757/ /pubmed/11429056 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01146.x Text en
spellingShingle Article
Hosokawa, Masashi
Sato, Akiyuki
Ishigamori, Hideki
Kohno, Hiroyuki
Tanaka, Takuji
Takahashi, Koretaro
Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate
title Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate
title_full Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate
title_fullStr Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate
title_full_unstemmed Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate
title_short Synergistic Effects of Highly Unsaturated Fatty Acid‐containing Phosphatidyl‐ethanolamine on Differentiation of Human Leukemia HL‐60 Cells by Dibutyryl Cyclic Adenosine Monophosphate
title_sort synergistic effects of highly unsaturated fatty acid‐containing phosphatidyl‐ethanolamine on differentiation of human leukemia hl‐60 cells by dibutyryl cyclic adenosine monophosphate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926757/
https://www.ncbi.nlm.nih.gov/pubmed/11429056
http://dx.doi.org/10.1111/j.1349-7006.2001.tb01146.x
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