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A Murine Osteosarcoma Cell Line with a Potential to Develop Ossification upon Transplantation

An Osteosarcoma cell line has been established from a soft tissue tumor that occurred spontaneously in a BALB/c mouse. This cell line showed ossification when transplanted into syngeneic mice. To examine the mechanism of bone formation, the expression of mRNAs for osteoblastic and chon‐droblastic ma...

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Autores principales: Kusumi, Tomomi, Nishi, Takashi, Tanaka, Masanori, Tsuchida, Shigeki, Kudo, Hajime
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926767/
https://www.ncbi.nlm.nih.gov/pubmed/11429054
http://dx.doi.org/10.1111/j.1349-7006.2001.tb01144.x
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author Kusumi, Tomomi
Nishi, Takashi
Tanaka, Masanori
Tsuchida, Shigeki
Kudo, Hajime
author_facet Kusumi, Tomomi
Nishi, Takashi
Tanaka, Masanori
Tsuchida, Shigeki
Kudo, Hajime
author_sort Kusumi, Tomomi
collection PubMed
description An Osteosarcoma cell line has been established from a soft tissue tumor that occurred spontaneously in a BALB/c mouse. This cell line showed ossification when transplanted into syngeneic mice. To examine the mechanism of bone formation, the expression of mRNAs for osteoblastic and chon‐droblastic markers and factors associated with ossification has been investigated. In culture, the cells exhibited a spindle shape in the growth phase, but had a polygonal shape in the stationary phase. Reverse transcription‐polymerase chain reaction analysis showed that the cells expressed mRNAs for pro‐α(I) chain of type I collagen, alkaline phosphatase, osteopontin, osteocalcin, and core binding factor al, suggesting differentiation into the stage of osteoblasts during the stationary phase. After transplantation, histological examination revealed small foci of pale blue material and basophilic networks that were scattered in the tumor tissues at one week. The former stained positive with alcian blue, suggesting a chondroid matrix. Pro‐α(II) chain of type II collagen mRNA was expressed at one week. A large part of tumors at two and three weeks consisted of basophilic networks, which stained positive via von Kossa's method, indicating a calcified woven bone. In situ hybridization analysis showed strong expression of osteopontin and osteocalcin mRNAs in tumor cells surrounding the bone matrix. Bone morphogenetic protein‐6 and ‐7 mRNAs were detected in transplanted tumors, but not in cultured cells. These results suggest that the cell line has the properties of an osteoblastic lineage when cultured in vitro and has an ossifying ability through endochondral bone formation processes when transplanted in vivo.
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spelling pubmed-59267672018-05-11 A Murine Osteosarcoma Cell Line with a Potential to Develop Ossification upon Transplantation Kusumi, Tomomi Nishi, Takashi Tanaka, Masanori Tsuchida, Shigeki Kudo, Hajime Jpn J Cancer Res Article An Osteosarcoma cell line has been established from a soft tissue tumor that occurred spontaneously in a BALB/c mouse. This cell line showed ossification when transplanted into syngeneic mice. To examine the mechanism of bone formation, the expression of mRNAs for osteoblastic and chon‐droblastic markers and factors associated with ossification has been investigated. In culture, the cells exhibited a spindle shape in the growth phase, but had a polygonal shape in the stationary phase. Reverse transcription‐polymerase chain reaction analysis showed that the cells expressed mRNAs for pro‐α(I) chain of type I collagen, alkaline phosphatase, osteopontin, osteocalcin, and core binding factor al, suggesting differentiation into the stage of osteoblasts during the stationary phase. After transplantation, histological examination revealed small foci of pale blue material and basophilic networks that were scattered in the tumor tissues at one week. The former stained positive with alcian blue, suggesting a chondroid matrix. Pro‐α(II) chain of type II collagen mRNA was expressed at one week. A large part of tumors at two and three weeks consisted of basophilic networks, which stained positive via von Kossa's method, indicating a calcified woven bone. In situ hybridization analysis showed strong expression of osteopontin and osteocalcin mRNAs in tumor cells surrounding the bone matrix. Bone morphogenetic protein‐6 and ‐7 mRNAs were detected in transplanted tumors, but not in cultured cells. These results suggest that the cell line has the properties of an osteoblastic lineage when cultured in vitro and has an ossifying ability through endochondral bone formation processes when transplanted in vivo. Blackwell Publishing Ltd 2001-06 /pmc/articles/PMC5926767/ /pubmed/11429054 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01144.x Text en
spellingShingle Article
Kusumi, Tomomi
Nishi, Takashi
Tanaka, Masanori
Tsuchida, Shigeki
Kudo, Hajime
A Murine Osteosarcoma Cell Line with a Potential to Develop Ossification upon Transplantation
title A Murine Osteosarcoma Cell Line with a Potential to Develop Ossification upon Transplantation
title_full A Murine Osteosarcoma Cell Line with a Potential to Develop Ossification upon Transplantation
title_fullStr A Murine Osteosarcoma Cell Line with a Potential to Develop Ossification upon Transplantation
title_full_unstemmed A Murine Osteosarcoma Cell Line with a Potential to Develop Ossification upon Transplantation
title_short A Murine Osteosarcoma Cell Line with a Potential to Develop Ossification upon Transplantation
title_sort murine osteosarcoma cell line with a potential to develop ossification upon transplantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926767/
https://www.ncbi.nlm.nih.gov/pubmed/11429054
http://dx.doi.org/10.1111/j.1349-7006.2001.tb01144.x
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