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Detection and Cloning of a Protein Recognized by Anti‐human Prostate‐specific Antigen (PSA) Antibody in the Rat Ventral Prostate
Prostate‐specific antigen (PSA), a 33 kDa glycoprotein produced in the epithelium of the human prostate, has become established as a useful tumor marker for prostate cancer in man. Since reports of homologous proteins in animals other than primates have been lacking, the present investigation was ca...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926826/ https://www.ncbi.nlm.nih.gov/pubmed/11509118 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01173.x |
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author | Onozawa, Mizuki Fukuda, Kazunori Watanabe, Masahiko Ohtani, Mikinobu Akaza, Hideyuki Sugimura, Takashi Wakabayashi, Keiji |
author_facet | Onozawa, Mizuki Fukuda, Kazunori Watanabe, Masahiko Ohtani, Mikinobu Akaza, Hideyuki Sugimura, Takashi Wakabayashi, Keiji |
author_sort | Onozawa, Mizuki |
collection | PubMed |
description | Prostate‐specific antigen (PSA), a 33 kDa glycoprotein produced in the epithelium of the human prostate, has become established as a useful tumor marker for prostate cancer in man. Since reports of homologous proteins in animals other than primates have been lacking, the present investigation was carried out to identify any PSA‐like protein in rats. Immunoblot analysis using a specific monoclonal anti‐human PSA antibody detected a 32 kDa immunoreactive protein in the ventral lobe of the rat prostate, but not in other lobes or in other tissues. Positive immunostaining was observed only for the luminal surface of the glandular epithelium and the intraductal fluid in the ventral prostate. Sequence analysis of a cDNA for the rat PSA‐like protein, cloned by immunoscreening of an expression cDNA library prepared from the ventral lobe, revealed identity to the rat submaxillary gland S3 kallikrein. Human PSA also belongs to the kallikrein family. Thus, this protein produced in the rat ventral prostate was suggested to be a possible counterpart of human PSA. |
format | Online Article Text |
id | pubmed-5926826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59268262018-05-11 Detection and Cloning of a Protein Recognized by Anti‐human Prostate‐specific Antigen (PSA) Antibody in the Rat Ventral Prostate Onozawa, Mizuki Fukuda, Kazunori Watanabe, Masahiko Ohtani, Mikinobu Akaza, Hideyuki Sugimura, Takashi Wakabayashi, Keiji Jpn J Cancer Res Article Prostate‐specific antigen (PSA), a 33 kDa glycoprotein produced in the epithelium of the human prostate, has become established as a useful tumor marker for prostate cancer in man. Since reports of homologous proteins in animals other than primates have been lacking, the present investigation was carried out to identify any PSA‐like protein in rats. Immunoblot analysis using a specific monoclonal anti‐human PSA antibody detected a 32 kDa immunoreactive protein in the ventral lobe of the rat prostate, but not in other lobes or in other tissues. Positive immunostaining was observed only for the luminal surface of the glandular epithelium and the intraductal fluid in the ventral prostate. Sequence analysis of a cDNA for the rat PSA‐like protein, cloned by immunoscreening of an expression cDNA library prepared from the ventral lobe, revealed identity to the rat submaxillary gland S3 kallikrein. Human PSA also belongs to the kallikrein family. Thus, this protein produced in the rat ventral prostate was suggested to be a possible counterpart of human PSA. Blackwell Publishing Ltd 2001-08 /pmc/articles/PMC5926826/ /pubmed/11509118 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01173.x Text en |
spellingShingle | Article Onozawa, Mizuki Fukuda, Kazunori Watanabe, Masahiko Ohtani, Mikinobu Akaza, Hideyuki Sugimura, Takashi Wakabayashi, Keiji Detection and Cloning of a Protein Recognized by Anti‐human Prostate‐specific Antigen (PSA) Antibody in the Rat Ventral Prostate |
title | Detection and Cloning of a Protein Recognized by Anti‐human Prostate‐specific Antigen (PSA) Antibody in the Rat Ventral Prostate |
title_full | Detection and Cloning of a Protein Recognized by Anti‐human Prostate‐specific Antigen (PSA) Antibody in the Rat Ventral Prostate |
title_fullStr | Detection and Cloning of a Protein Recognized by Anti‐human Prostate‐specific Antigen (PSA) Antibody in the Rat Ventral Prostate |
title_full_unstemmed | Detection and Cloning of a Protein Recognized by Anti‐human Prostate‐specific Antigen (PSA) Antibody in the Rat Ventral Prostate |
title_short | Detection and Cloning of a Protein Recognized by Anti‐human Prostate‐specific Antigen (PSA) Antibody in the Rat Ventral Prostate |
title_sort | detection and cloning of a protein recognized by anti‐human prostate‐specific antigen (psa) antibody in the rat ventral prostate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926826/ https://www.ncbi.nlm.nih.gov/pubmed/11509118 http://dx.doi.org/10.1111/j.1349-7006.2001.tb01173.x |
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