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Estrogen Receptor Expression and Estrogen Receptor‐independent Cytotoxic Effects of Tamoxifen on Malignant Rhabdoid Tumor Cells in vitro

Recent studies have shown that the antiestrogen tamoxifen (TAM) can be used in the treatment of malignant neoplasms other than breast cancer. In the present study, we investigated the expression of estrogen receptor (ER) in six malignant rhabdoid tumor (MRT) cell lines. Alterations in MRT cell growt...

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Autores principales: Koshida, Shigeki, Narita, Tsutomu, Kato, Hirofumi, Yoshida, Shinobu, Taga, Takashi, Ohta, Shigeru, Takeuchi, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926929/
https://www.ncbi.nlm.nih.gov/pubmed/12495475
http://dx.doi.org/10.1111/j.1349-7006.2002.tb01244.x
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author Koshida, Shigeki
Narita, Tsutomu
Kato, Hirofumi
Yoshida, Shinobu
Taga, Takashi
Ohta, Shigeru
Takeuchi, Yoshihiro
author_facet Koshida, Shigeki
Narita, Tsutomu
Kato, Hirofumi
Yoshida, Shinobu
Taga, Takashi
Ohta, Shigeru
Takeuchi, Yoshihiro
author_sort Koshida, Shigeki
collection PubMed
description Recent studies have shown that the antiestrogen tamoxifen (TAM) can be used in the treatment of malignant neoplasms other than breast cancer. In the present study, we investigated the expression of estrogen receptor (ER) in six malignant rhabdoid tumor (MRT) cell lines. Alterations in MRT cell growth in response to estrogen or antiestrogens (4‐hydroxytamoxifen (4‐OHT), TAM, and ICI 182 780) were also investigated. RT‐PCR and western blotting showed that ER‐a was expressed in three of the six MRT cell lines. While 17‐β‐estradiol (E2) did not significantly alter MRT cell line proliferation, the hydroxylated tamoxifen metabolite 4‐OHT significantly inhibited the growth of all 6 MRT cell lines. However, the steroidal antiestrogen ICI 182 780 did not alter the proliferation of any of the MRT cell lines. 4‐OHT induced apoptosis in both ER‐α‐negative and ER‐α‐positive MRT cell lines, as assessed by nuclear morphology and DNA fragmentation. Neither growth inhibition nor induction of apoptosis due to 4‐OHT was blocked by the addition of excess E2. Our data suggested that 4‐OHT induced cytotoxic effects against MRT cells, and that these effects were independent of ER expression.
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spelling pubmed-59269292018-05-11 Estrogen Receptor Expression and Estrogen Receptor‐independent Cytotoxic Effects of Tamoxifen on Malignant Rhabdoid Tumor Cells in vitro Koshida, Shigeki Narita, Tsutomu Kato, Hirofumi Yoshida, Shinobu Taga, Takashi Ohta, Shigeru Takeuchi, Yoshihiro Jpn J Cancer Res Article Recent studies have shown that the antiestrogen tamoxifen (TAM) can be used in the treatment of malignant neoplasms other than breast cancer. In the present study, we investigated the expression of estrogen receptor (ER) in six malignant rhabdoid tumor (MRT) cell lines. Alterations in MRT cell growth in response to estrogen or antiestrogens (4‐hydroxytamoxifen (4‐OHT), TAM, and ICI 182 780) were also investigated. RT‐PCR and western blotting showed that ER‐a was expressed in three of the six MRT cell lines. While 17‐β‐estradiol (E2) did not significantly alter MRT cell line proliferation, the hydroxylated tamoxifen metabolite 4‐OHT significantly inhibited the growth of all 6 MRT cell lines. However, the steroidal antiestrogen ICI 182 780 did not alter the proliferation of any of the MRT cell lines. 4‐OHT induced apoptosis in both ER‐α‐negative and ER‐α‐positive MRT cell lines, as assessed by nuclear morphology and DNA fragmentation. Neither growth inhibition nor induction of apoptosis due to 4‐OHT was blocked by the addition of excess E2. Our data suggested that 4‐OHT induced cytotoxic effects against MRT cells, and that these effects were independent of ER expression. Blackwell Publishing Ltd 2002-12 /pmc/articles/PMC5926929/ /pubmed/12495475 http://dx.doi.org/10.1111/j.1349-7006.2002.tb01244.x Text en
spellingShingle Article
Koshida, Shigeki
Narita, Tsutomu
Kato, Hirofumi
Yoshida, Shinobu
Taga, Takashi
Ohta, Shigeru
Takeuchi, Yoshihiro
Estrogen Receptor Expression and Estrogen Receptor‐independent Cytotoxic Effects of Tamoxifen on Malignant Rhabdoid Tumor Cells in vitro
title Estrogen Receptor Expression and Estrogen Receptor‐independent Cytotoxic Effects of Tamoxifen on Malignant Rhabdoid Tumor Cells in vitro
title_full Estrogen Receptor Expression and Estrogen Receptor‐independent Cytotoxic Effects of Tamoxifen on Malignant Rhabdoid Tumor Cells in vitro
title_fullStr Estrogen Receptor Expression and Estrogen Receptor‐independent Cytotoxic Effects of Tamoxifen on Malignant Rhabdoid Tumor Cells in vitro
title_full_unstemmed Estrogen Receptor Expression and Estrogen Receptor‐independent Cytotoxic Effects of Tamoxifen on Malignant Rhabdoid Tumor Cells in vitro
title_short Estrogen Receptor Expression and Estrogen Receptor‐independent Cytotoxic Effects of Tamoxifen on Malignant Rhabdoid Tumor Cells in vitro
title_sort estrogen receptor expression and estrogen receptor‐independent cytotoxic effects of tamoxifen on malignant rhabdoid tumor cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926929/
https://www.ncbi.nlm.nih.gov/pubmed/12495475
http://dx.doi.org/10.1111/j.1349-7006.2002.tb01244.x
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