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Antitumor Effect of an Adeno‐associated Virus Vector Containing the Human Interferon‐β Gene on Experimental Intracranial Human Glioma
We constructed an adeno‐associated virus (AAV) vector containing the human interferon‐β(HuIFN‐β) gene (AAV‐IFN‐β) and investigated its antitumor effect against human glioma cells (U251‐SP) inoculated into the brain of nude mice. Prior to this, we examined human glioma cells transduced with AAV‐IFN‐β...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926956/ https://www.ncbi.nlm.nih.gov/pubmed/11856487 http://dx.doi.org/10.1111/j.1349-7006.2002.tb01262.x |
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author | Yoshida, Jun Mizuno, Masaaki Nakahara, Norimoto Colosi, Peter |
author_facet | Yoshida, Jun Mizuno, Masaaki Nakahara, Norimoto Colosi, Peter |
author_sort | Yoshida, Jun |
collection | PubMed |
description | We constructed an adeno‐associated virus (AAV) vector containing the human interferon‐β(HuIFN‐β) gene (AAV‐IFN‐β) and investigated its antitumor effect against human glioma cells (U251‐SP) inoculated into the brain of nude mice. Prior to this, we examined human glioma cells transduced with AAV‐IFN‐β using video‐enhanced contrast differential interference contrast (VEC‐DIC) microscopy. Infection of AAV‐IFN‐β induced apoptosis and secondary necrosis in human glioma cells. In in vivo experiments, we confirmed production of HuIFN‐β and induction of heat‐shock protein (HSP) in glioma cells transduced with AAV‐IFN‐β. Growth of the experimental gliomas was completely inhibited by six injections of AAV‐IFN‐β, starting 7 days after transplantation of glioma cells. In addition, the survival of mice treated with AAV‐IFN‐β was remarkably prolonged. These results indicate that AAV‐IFN‐β induces apoptosis of glioma cells and has a strong antitumor effect in this experimental glioma model. |
format | Online Article Text |
id | pubmed-5926956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59269562018-05-11 Antitumor Effect of an Adeno‐associated Virus Vector Containing the Human Interferon‐β Gene on Experimental Intracranial Human Glioma Yoshida, Jun Mizuno, Masaaki Nakahara, Norimoto Colosi, Peter Jpn J Cancer Res Article We constructed an adeno‐associated virus (AAV) vector containing the human interferon‐β(HuIFN‐β) gene (AAV‐IFN‐β) and investigated its antitumor effect against human glioma cells (U251‐SP) inoculated into the brain of nude mice. Prior to this, we examined human glioma cells transduced with AAV‐IFN‐β using video‐enhanced contrast differential interference contrast (VEC‐DIC) microscopy. Infection of AAV‐IFN‐β induced apoptosis and secondary necrosis in human glioma cells. In in vivo experiments, we confirmed production of HuIFN‐β and induction of heat‐shock protein (HSP) in glioma cells transduced with AAV‐IFN‐β. Growth of the experimental gliomas was completely inhibited by six injections of AAV‐IFN‐β, starting 7 days after transplantation of glioma cells. In addition, the survival of mice treated with AAV‐IFN‐β was remarkably prolonged. These results indicate that AAV‐IFN‐β induces apoptosis of glioma cells and has a strong antitumor effect in this experimental glioma model. Blackwell Publishing Ltd 2002-02 /pmc/articles/PMC5926956/ /pubmed/11856487 http://dx.doi.org/10.1111/j.1349-7006.2002.tb01262.x Text en |
spellingShingle | Article Yoshida, Jun Mizuno, Masaaki Nakahara, Norimoto Colosi, Peter Antitumor Effect of an Adeno‐associated Virus Vector Containing the Human Interferon‐β Gene on Experimental Intracranial Human Glioma |
title | Antitumor Effect of an Adeno‐associated Virus Vector Containing the Human Interferon‐β Gene on Experimental Intracranial Human Glioma |
title_full | Antitumor Effect of an Adeno‐associated Virus Vector Containing the Human Interferon‐β Gene on Experimental Intracranial Human Glioma |
title_fullStr | Antitumor Effect of an Adeno‐associated Virus Vector Containing the Human Interferon‐β Gene on Experimental Intracranial Human Glioma |
title_full_unstemmed | Antitumor Effect of an Adeno‐associated Virus Vector Containing the Human Interferon‐β Gene on Experimental Intracranial Human Glioma |
title_short | Antitumor Effect of an Adeno‐associated Virus Vector Containing the Human Interferon‐β Gene on Experimental Intracranial Human Glioma |
title_sort | antitumor effect of an adeno‐associated virus vector containing the human interferon‐β gene on experimental intracranial human glioma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926956/ https://www.ncbi.nlm.nih.gov/pubmed/11856487 http://dx.doi.org/10.1111/j.1349-7006.2002.tb01262.x |
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