Multiplexed Analysis of Post‐PCR Fluorescence‐labeled Microsatellite Alleles and Statistical Evaluation of Their Imbalance in Brain Tumors

Detection of the loss of chromosomal regions in cancerous tissues has diagnostic and prognostic relevance, and the development of a reliable and cost‐effective technique for this is clinically important. Here we present an efficient technique for quantitative detection of microsatellite alleles, using a post‐PCR fluorescence‐labeling procedure and multiplexed analysis. We also present a new statistical method for the interpretation of the data that permits reliable and sensitive evaluation of the allelic status of sampled DNA. A high‐resolution analysis of allelic imbalance on chromosomes 1p, 10 and 19q in 28 glioma samples of various types using this method revealed that allelic imbalances are more frequent than have been reported, suggesting the diagnostic value of this method in examining the genetic profiles of gliomas.