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Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications
BACKGROUND: The luminescence amplification of semiconductor quantum dots (QD) in the presence of self-assembled gold nanoparticles (Au NPs) is one of way for creating biosensors with highly efficient transduction. AIMS: The objective of this study was to fabricate the hybrid structures based on semi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927191/ https://www.ncbi.nlm.nih.gov/pubmed/29731613 http://dx.doi.org/10.2147/NSA.S155045 |
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author | Kurochkina, Margarita Konshina, Elena Oseev, Aleksandr Hirsch, Soeren |
author_facet | Kurochkina, Margarita Konshina, Elena Oseev, Aleksandr Hirsch, Soeren |
author_sort | Kurochkina, Margarita |
collection | PubMed |
description | BACKGROUND: The luminescence amplification of semiconductor quantum dots (QD) in the presence of self-assembled gold nanoparticles (Au NPs) is one of way for creating biosensors with highly efficient transduction. AIMS: The objective of this study was to fabricate the hybrid structures based on semiconductor CdSe/ZnS QDs and Au NP arrays and to use them as biosensors of protein. METHODS: In this paper, the hybrid structures based on CdSe/ZnS QDs and Au NP arrays were fabricated using spin coating processes. Au NP arrays deposited on a glass wafer were investigated by optical microscopy and absorption spectroscopy depending on numbers of spin coating layers and their baking temperature. Bovine serum albumin (BSA) was used as the target protein analyte in a phosphate buffer. A confocal laser scanning microscope was used to study the luminescent properties of Au NP/QD hybrid structures and to test BSA. RESULTS: The dimensions of Au NP aggregates increased and the space between them decreased with increasing processing temperature. At the same time, a blue shift of the plasmon resonance peak in the absorption spectra of Au NP arrays was observed. The deposition of CdSe/ZnS QDs with a core diameter of 5 nm on the surface of the Au NP arrays caused an increase in absorption and a red shift of the plasmon peak in the spectra. The exciton–plasmon enhancement of the QDs’ photoluminescence intensity has been obtained at room temperature for hybrid structures with Au NPs array pretreated at temperatures of 100°C and 150°C. It has been found that an increase in the weight content of BSA increases the photoluminescence intensity of such hybrid structures. CONCLUSION: The ability of the qualitative and quantitative determination of protein content in solution using the Au NP/QD structures as an optical biosensor has been shown experimentally. |
format | Online Article Text |
id | pubmed-5927191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59271912018-05-04 Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications Kurochkina, Margarita Konshina, Elena Oseev, Aleksandr Hirsch, Soeren Nanotechnol Sci Appl Original Research BACKGROUND: The luminescence amplification of semiconductor quantum dots (QD) in the presence of self-assembled gold nanoparticles (Au NPs) is one of way for creating biosensors with highly efficient transduction. AIMS: The objective of this study was to fabricate the hybrid structures based on semiconductor CdSe/ZnS QDs and Au NP arrays and to use them as biosensors of protein. METHODS: In this paper, the hybrid structures based on CdSe/ZnS QDs and Au NP arrays were fabricated using spin coating processes. Au NP arrays deposited on a glass wafer were investigated by optical microscopy and absorption spectroscopy depending on numbers of spin coating layers and their baking temperature. Bovine serum albumin (BSA) was used as the target protein analyte in a phosphate buffer. A confocal laser scanning microscope was used to study the luminescent properties of Au NP/QD hybrid structures and to test BSA. RESULTS: The dimensions of Au NP aggregates increased and the space between them decreased with increasing processing temperature. At the same time, a blue shift of the plasmon resonance peak in the absorption spectra of Au NP arrays was observed. The deposition of CdSe/ZnS QDs with a core diameter of 5 nm on the surface of the Au NP arrays caused an increase in absorption and a red shift of the plasmon peak in the spectra. The exciton–plasmon enhancement of the QDs’ photoluminescence intensity has been obtained at room temperature for hybrid structures with Au NPs array pretreated at temperatures of 100°C and 150°C. It has been found that an increase in the weight content of BSA increases the photoluminescence intensity of such hybrid structures. CONCLUSION: The ability of the qualitative and quantitative determination of protein content in solution using the Au NP/QD structures as an optical biosensor has been shown experimentally. Dove Medical Press 2018-04-27 /pmc/articles/PMC5927191/ /pubmed/29731613 http://dx.doi.org/10.2147/NSA.S155045 Text en © 2018 Kurochkina et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Kurochkina, Margarita Konshina, Elena Oseev, Aleksandr Hirsch, Soeren Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications |
title | Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications |
title_full | Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications |
title_fullStr | Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications |
title_full_unstemmed | Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications |
title_short | Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications |
title_sort | hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927191/ https://www.ncbi.nlm.nih.gov/pubmed/29731613 http://dx.doi.org/10.2147/NSA.S155045 |
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