Nanosized titanium dioxide-induced premature ovarian failure is associated with abnormalities in serum parameters in female mice

BACKGROUND: Exposure to titanium dioxide nanoparticles (TiO(2) NPs) that are widely used in food, medicine, sunscreen products and cosmetics is reported to cause ovarian damage and lower fertility in animals. However, the potential effects of TiO(2) NPs application on premature ovarian failure (POF) have rarely been evaluated to date. METHODS: In this study, female mice were continuously exposed to TiO(2) NPs at doses of 2.5, 5 or 10 mg/kg via gavage instillation for 30 days, and investigated the serum hormones and autoimmunity markers associated with POF. RESULTS: Exposure to TiO(2) NPs resulted in POF, reductions in the levels of estradiol, progesterone and inhibin B and increases in luteinizing hormone, follicle-stimulating hormone, follicle-stimulating hormone/luteinizing hormone ratio, anti-Müllerian hormone, thyroid-stimulating hormone, free triiodothyronine, free tetraiodothyronine, anti-nuclear antibody and anti-thyroid peroxidase antibody levels in serum. CONCLUSION: Exposure to TiO(2) NPs induced POF triggered by alterations in hormones and autoimmunity markers. Our findings highlight the necessity for significant caution in handling and usage of TiO(2) NPs by female consumers.