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A comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings

Understanding the functional basis of auxin homeostasis requires knowledge about auxin biosynthesis, auxin transport and auxin catabolism genes, which is not always directly available despite the recent whole-genome sequencing of many plant species. Through sequence homology searches and phylogeneti...

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Autores principales: Sánchez-García, Ana Belén, Ibáñez, Sergio, Cano, Antonio, Acosta, Manuel, Pérez-Pérez, José Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927418/
https://www.ncbi.nlm.nih.gov/pubmed/29709027
http://dx.doi.org/10.1371/journal.pone.0196663
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author Sánchez-García, Ana Belén
Ibáñez, Sergio
Cano, Antonio
Acosta, Manuel
Pérez-Pérez, José Manuel
author_facet Sánchez-García, Ana Belén
Ibáñez, Sergio
Cano, Antonio
Acosta, Manuel
Pérez-Pérez, José Manuel
author_sort Sánchez-García, Ana Belén
collection PubMed
description Understanding the functional basis of auxin homeostasis requires knowledge about auxin biosynthesis, auxin transport and auxin catabolism genes, which is not always directly available despite the recent whole-genome sequencing of many plant species. Through sequence homology searches and phylogenetic analyses on a selection of 11 plant species with high-quality genome annotation, we identified the putative gene homologs involved in auxin biosynthesis, auxin catabolism and auxin transport pathways in carnation (Dianthus caryophyllus L.). To deepen our knowledge of the regulatory events underlying auxin-mediated adventitious root formation in carnation stem cuttings, we used RNA-sequencing data to confirm the expression profiles of some auxin homeostasis genes during the rooting of two carnation cultivars with different rooting behaviors. We also confirmed the presence of several auxin-related metabolites in the stem cutting tissues. Our findings offer a comprehensive overview of auxin homeostasis genes in carnation and provide a solid foundation for further experiments investigating the role of auxin homeostasis in the regulation of adventitious root formation in carnation.
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spelling pubmed-59274182018-05-11 A comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings Sánchez-García, Ana Belén Ibáñez, Sergio Cano, Antonio Acosta, Manuel Pérez-Pérez, José Manuel PLoS One Research Article Understanding the functional basis of auxin homeostasis requires knowledge about auxin biosynthesis, auxin transport and auxin catabolism genes, which is not always directly available despite the recent whole-genome sequencing of many plant species. Through sequence homology searches and phylogenetic analyses on a selection of 11 plant species with high-quality genome annotation, we identified the putative gene homologs involved in auxin biosynthesis, auxin catabolism and auxin transport pathways in carnation (Dianthus caryophyllus L.). To deepen our knowledge of the regulatory events underlying auxin-mediated adventitious root formation in carnation stem cuttings, we used RNA-sequencing data to confirm the expression profiles of some auxin homeostasis genes during the rooting of two carnation cultivars with different rooting behaviors. We also confirmed the presence of several auxin-related metabolites in the stem cutting tissues. Our findings offer a comprehensive overview of auxin homeostasis genes in carnation and provide a solid foundation for further experiments investigating the role of auxin homeostasis in the regulation of adventitious root formation in carnation. Public Library of Science 2018-04-30 /pmc/articles/PMC5927418/ /pubmed/29709027 http://dx.doi.org/10.1371/journal.pone.0196663 Text en © 2018 Sánchez-García et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sánchez-García, Ana Belén
Ibáñez, Sergio
Cano, Antonio
Acosta, Manuel
Pérez-Pérez, José Manuel
A comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings
title A comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings
title_full A comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings
title_fullStr A comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings
title_full_unstemmed A comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings
title_short A comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings
title_sort comprehensive phylogeny of auxin homeostasis genes involved in adventitious root formation in carnation stem cuttings
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927418/
https://www.ncbi.nlm.nih.gov/pubmed/29709027
http://dx.doi.org/10.1371/journal.pone.0196663
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