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Measuring dynamics of eukaryotic transcription initiation: Challenges, insights and opportunities

Transcription of protein-encoding genes in eukaryotic cells is a dynamically coordinated process. Many of the key transcription regulators contain functionally essential intrinsically disordered regions (IDRs), the dynamic nature of which creates extra challenges to traditional biochemical analyses....

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Detalles Bibliográficos
Autores principales: Zhang, Zhengjian, Tjian, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927711/
https://www.ncbi.nlm.nih.gov/pubmed/28920762
http://dx.doi.org/10.1080/21541264.2017.1363017
Descripción
Sumario:Transcription of protein-encoding genes in eukaryotic cells is a dynamically coordinated process. Many of the key transcription regulators contain functionally essential intrinsically disordered regions (IDRs), the dynamic nature of which creates extra challenges to traditional biochemical analyses. Recent advances in single-molecule fluorescence imaging technology have enabled direct visualization of these rapid, complex and dynamic molecular interactions in real time.