Measuring dynamics of eukaryotic transcription initiation: Challenges, insights and opportunities

Transcription of protein-encoding genes in eukaryotic cells is a dynamically coordinated process. Many of the key transcription regulators contain functionally essential intrinsically disordered regions (IDRs), the dynamic nature of which creates extra challenges to traditional biochemical analyses....

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Detalles Bibliográficos
Autores principales: Zhang, Zhengjian, Tjian, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Point-of-View
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927711/
https://www.ncbi.nlm.nih.gov/pubmed/28920762
http://dx.doi.org/10.1080/21541264.2017.1363017
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author Zhang, Zhengjian
Tjian, Robert
author_facet Zhang, Zhengjian
Tjian, Robert
author_sort Zhang, Zhengjian
collection PubMed
description Transcription of protein-encoding genes in eukaryotic cells is a dynamically coordinated process. Many of the key transcription regulators contain functionally essential intrinsically disordered regions (IDRs), the dynamic nature of which creates extra challenges to traditional biochemical analyses. Recent advances in single-molecule fluorescence imaging technology have enabled direct visualization of these rapid, complex and dynamic molecular interactions in real time.
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spelling pubmed-59277112018-05-02 Measuring dynamics of eukaryotic transcription initiation: Challenges, insights and opportunities Zhang, Zhengjian Tjian, Robert Transcription Point-of-View Transcription of protein-encoding genes in eukaryotic cells is a dynamically coordinated process. Many of the key transcription regulators contain functionally essential intrinsically disordered regions (IDRs), the dynamic nature of which creates extra challenges to traditional biochemical analyses. Recent advances in single-molecule fluorescence imaging technology have enabled direct visualization of these rapid, complex and dynamic molecular interactions in real time. Taylor & Francis 2017-10-09 /pmc/articles/PMC5927711/ /pubmed/28920762 http://dx.doi.org/10.1080/21541264.2017.1363017 Text en © 2017 Zhengjian Zhang and Robert Tjian. Published with license by Taylor & Francis. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Point-of-View
Zhang, Zhengjian
Tjian, Robert
Measuring dynamics of eukaryotic transcription initiation: Challenges, insights and opportunities
title Measuring dynamics of eukaryotic transcription initiation: Challenges, insights and opportunities
title_full Measuring dynamics of eukaryotic transcription initiation: Challenges, insights and opportunities
title_fullStr Measuring dynamics of eukaryotic transcription initiation: Challenges, insights and opportunities
title_full_unstemmed Measuring dynamics of eukaryotic transcription initiation: Challenges, insights and opportunities
title_short Measuring dynamics of eukaryotic transcription initiation: Challenges, insights and opportunities
title_sort measuring dynamics of eukaryotic transcription initiation: challenges, insights and opportunities
topic Point-of-View
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927711/
https://www.ncbi.nlm.nih.gov/pubmed/28920762
http://dx.doi.org/10.1080/21541264.2017.1363017
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