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Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation

Postsynaptic compartments can be specifically modulated during various forms of synaptic plasticity, but it is unclear whether this precision is shared at presynaptic terminals. Presynaptic homeostatic plasticity (PHP) stabilizes neurotransmission at the Drosophila neuromuscular junction, where a re...

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Autores principales: Li, Xiling, Goel, Pragya, Chen, Catherine, Angajala, Varun, Chen, Xun, Dickman, Dion K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927770/
https://www.ncbi.nlm.nih.gov/pubmed/29620520
http://dx.doi.org/10.7554/eLife.34338
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author Li, Xiling
Goel, Pragya
Chen, Catherine
Angajala, Varun
Chen, Xun
Dickman, Dion K
author_facet Li, Xiling
Goel, Pragya
Chen, Catherine
Angajala, Varun
Chen, Xun
Dickman, Dion K
author_sort Li, Xiling
collection PubMed
description Postsynaptic compartments can be specifically modulated during various forms of synaptic plasticity, but it is unclear whether this precision is shared at presynaptic terminals. Presynaptic homeostatic plasticity (PHP) stabilizes neurotransmission at the Drosophila neuromuscular junction, where a retrograde enhancement of presynaptic neurotransmitter release compensates for diminished postsynaptic receptor functionality. To test the specificity of PHP induction and expression, we have developed a genetic manipulation to reduce postsynaptic receptor expression at one of the two muscles innervated by a single motor neuron. We find that PHP can be induced and expressed at a subset of synapses, over both acute and chronic time scales, without influencing transmission at adjacent release sites. Further, homeostatic modulations to CaMKII, vesicle pools, and functional release sites are compartmentalized and do not spread to neighboring pre- or post-synaptic structures. Thus, both PHP induction and expression mechanisms are locally transmitted and restricted to specific synaptic compartments.
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spelling pubmed-59277702018-05-02 Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation Li, Xiling Goel, Pragya Chen, Catherine Angajala, Varun Chen, Xun Dickman, Dion K eLife Neuroscience Postsynaptic compartments can be specifically modulated during various forms of synaptic plasticity, but it is unclear whether this precision is shared at presynaptic terminals. Presynaptic homeostatic plasticity (PHP) stabilizes neurotransmission at the Drosophila neuromuscular junction, where a retrograde enhancement of presynaptic neurotransmitter release compensates for diminished postsynaptic receptor functionality. To test the specificity of PHP induction and expression, we have developed a genetic manipulation to reduce postsynaptic receptor expression at one of the two muscles innervated by a single motor neuron. We find that PHP can be induced and expressed at a subset of synapses, over both acute and chronic time scales, without influencing transmission at adjacent release sites. Further, homeostatic modulations to CaMKII, vesicle pools, and functional release sites are compartmentalized and do not spread to neighboring pre- or post-synaptic structures. Thus, both PHP induction and expression mechanisms are locally transmitted and restricted to specific synaptic compartments. eLife Sciences Publications, Ltd 2018-04-05 /pmc/articles/PMC5927770/ /pubmed/29620520 http://dx.doi.org/10.7554/eLife.34338 Text en © 2018, Li et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Li, Xiling
Goel, Pragya
Chen, Catherine
Angajala, Varun
Chen, Xun
Dickman, Dion K
Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation
title Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation
title_full Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation
title_fullStr Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation
title_full_unstemmed Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation
title_short Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation
title_sort synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927770/
https://www.ncbi.nlm.nih.gov/pubmed/29620520
http://dx.doi.org/10.7554/eLife.34338
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