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Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction

HIV infection and type 2 diabetes are associated with altered gut microbiota, chronic inflammation, and increased cardiovascular risk. We aimed to investigate the combined effect of these diseases on gut microbiota composition and related metabolites, and a potential relation to endothelial dysfunct...

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Autores principales: Hoel, Hedda, Hove-Skovsgaard, Malene, Hov, Johannes R., Gaardbo, Julie Christine, Holm, Kristian, Kummen, Martin, Rudi, Knut, Nwosu, Felix, Valeur, Jørgen, Gelpi, Marco, Seljeflot, Ingebjørg, Ueland, Per Magne, Gerstoft, Jan, Ullum, Henrik, Aukrust, Pål, Nielsen, Susanne Dam, Trøseid, Marius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928109/
https://www.ncbi.nlm.nih.gov/pubmed/29712976
http://dx.doi.org/10.1038/s41598-018-25168-3
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author Hoel, Hedda
Hove-Skovsgaard, Malene
Hov, Johannes R.
Gaardbo, Julie Christine
Holm, Kristian
Kummen, Martin
Rudi, Knut
Nwosu, Felix
Valeur, Jørgen
Gelpi, Marco
Seljeflot, Ingebjørg
Ueland, Per Magne
Gerstoft, Jan
Ullum, Henrik
Aukrust, Pål
Nielsen, Susanne Dam
Trøseid, Marius
author_facet Hoel, Hedda
Hove-Skovsgaard, Malene
Hov, Johannes R.
Gaardbo, Julie Christine
Holm, Kristian
Kummen, Martin
Rudi, Knut
Nwosu, Felix
Valeur, Jørgen
Gelpi, Marco
Seljeflot, Ingebjørg
Ueland, Per Magne
Gerstoft, Jan
Ullum, Henrik
Aukrust, Pål
Nielsen, Susanne Dam
Trøseid, Marius
author_sort Hoel, Hedda
collection PubMed
description HIV infection and type 2 diabetes are associated with altered gut microbiota, chronic inflammation, and increased cardiovascular risk. We aimed to investigate the combined effect of these diseases on gut microbiota composition and related metabolites, and a potential relation to endothelial dysfunction in individuals with HIV-infection only (n = 23), diabetes only (n = 16) or both conditions (n = 21), as well as controls (n = 24). Fecal microbiota was analyzed by Illumina sequencing of the 16 S rRNA gene. Markers of endothelial dysfunction (asymmetric dimethylarginine [ADMA]), tryptophan catabolism (kynurenine/tryptophan [KT]-ratio), and inflammation (neopterin) were measured by liquid chromatography-tandem mass spectrometry. The combination of HIV and type 2 diabetes was associated with reduced gut microbiota diversity, increased plasma KT-ratio and neopterin. Microbial genes related to tryptophan metabolism correlated with KT-ratio and low alpha diversity, in particular in HIV-infected with T2D. In multivariate analyses, KT-ratio associated with ADMA (β = 4.58 [95% CI 2.53–6.63], p < 0.001), whereas microbiota composition per se was not associated with endothelial dysfunction. Our results indicate that tryptophan catabolism may be related to endothelial dysfunction, with a potentially detrimental interaction between HIV and diabetes. The potential contribution of gut microbiota and the impact for cardiovascular risk should be further explored in prospective studies powered for clinical end points.
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spelling pubmed-59281092018-05-07 Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction Hoel, Hedda Hove-Skovsgaard, Malene Hov, Johannes R. Gaardbo, Julie Christine Holm, Kristian Kummen, Martin Rudi, Knut Nwosu, Felix Valeur, Jørgen Gelpi, Marco Seljeflot, Ingebjørg Ueland, Per Magne Gerstoft, Jan Ullum, Henrik Aukrust, Pål Nielsen, Susanne Dam Trøseid, Marius Sci Rep Article HIV infection and type 2 diabetes are associated with altered gut microbiota, chronic inflammation, and increased cardiovascular risk. We aimed to investigate the combined effect of these diseases on gut microbiota composition and related metabolites, and a potential relation to endothelial dysfunction in individuals with HIV-infection only (n = 23), diabetes only (n = 16) or both conditions (n = 21), as well as controls (n = 24). Fecal microbiota was analyzed by Illumina sequencing of the 16 S rRNA gene. Markers of endothelial dysfunction (asymmetric dimethylarginine [ADMA]), tryptophan catabolism (kynurenine/tryptophan [KT]-ratio), and inflammation (neopterin) were measured by liquid chromatography-tandem mass spectrometry. The combination of HIV and type 2 diabetes was associated with reduced gut microbiota diversity, increased plasma KT-ratio and neopterin. Microbial genes related to tryptophan metabolism correlated with KT-ratio and low alpha diversity, in particular in HIV-infected with T2D. In multivariate analyses, KT-ratio associated with ADMA (β = 4.58 [95% CI 2.53–6.63], p < 0.001), whereas microbiota composition per se was not associated with endothelial dysfunction. Our results indicate that tryptophan catabolism may be related to endothelial dysfunction, with a potentially detrimental interaction between HIV and diabetes. The potential contribution of gut microbiota and the impact for cardiovascular risk should be further explored in prospective studies powered for clinical end points. Nature Publishing Group UK 2018-04-30 /pmc/articles/PMC5928109/ /pubmed/29712976 http://dx.doi.org/10.1038/s41598-018-25168-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hoel, Hedda
Hove-Skovsgaard, Malene
Hov, Johannes R.
Gaardbo, Julie Christine
Holm, Kristian
Kummen, Martin
Rudi, Knut
Nwosu, Felix
Valeur, Jørgen
Gelpi, Marco
Seljeflot, Ingebjørg
Ueland, Per Magne
Gerstoft, Jan
Ullum, Henrik
Aukrust, Pål
Nielsen, Susanne Dam
Trøseid, Marius
Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction
title Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction
title_full Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction
title_fullStr Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction
title_full_unstemmed Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction
title_short Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction
title_sort impact of hiv and type 2 diabetes on gut microbiota diversity, tryptophan catabolism and endothelial dysfunction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928109/
https://www.ncbi.nlm.nih.gov/pubmed/29712976
http://dx.doi.org/10.1038/s41598-018-25168-3
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