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Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico

Aedes aegypti is the primary urban mosquito vector of viruses causing dengue, Zika and chikungunya fevers –for which vaccines and effective pharmaceuticals are still lacking. Current strategies to suppress arbovirus outbreaks include removal of larval-breeding sites and insecticide treatment of larv...

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Autores principales: Saavedra-Rodriguez, Karla, Maloof, Farah Vera, Campbell, Corey L., Garcia-Rejon, Julian, Lenhart, Audrey, Penilla, Patricia, Rodriguez, Americo, Sandoval, Arturo Acero, Flores, Adriana E., Ponce, Gustavo, Lozano, Saul, Black, William C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928250/
https://www.ncbi.nlm.nih.gov/pubmed/29712956
http://dx.doi.org/10.1038/s41598-018-25222-0
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author Saavedra-Rodriguez, Karla
Maloof, Farah Vera
Campbell, Corey L.
Garcia-Rejon, Julian
Lenhart, Audrey
Penilla, Patricia
Rodriguez, Americo
Sandoval, Arturo Acero
Flores, Adriana E.
Ponce, Gustavo
Lozano, Saul
Black, William C.
author_facet Saavedra-Rodriguez, Karla
Maloof, Farah Vera
Campbell, Corey L.
Garcia-Rejon, Julian
Lenhart, Audrey
Penilla, Patricia
Rodriguez, Americo
Sandoval, Arturo Acero
Flores, Adriana E.
Ponce, Gustavo
Lozano, Saul
Black, William C.
author_sort Saavedra-Rodriguez, Karla
collection PubMed
description Aedes aegypti is the primary urban mosquito vector of viruses causing dengue, Zika and chikungunya fevers –for which vaccines and effective pharmaceuticals are still lacking. Current strategies to suppress arbovirus outbreaks include removal of larval-breeding sites and insecticide treatment of larval and adult populations. Insecticidal control of Ae. aegypti is challenging, due to a recent rapid global increase in knockdown-resistance (kdr) to pyrethroid insecticides. Widespread, heavy use of pyrethroid space-sprays has created an immense selection pressure for kdr, which is primarily under the control of the voltage-gated sodium channel gene (vgsc). To date, eleven replacements in vgsc have been discovered, published and shown to be associated with pyrethroid resistance to varying degrees. In Mexico, F1,534C and V1,016I have co-evolved in the last 16 years across Ae. aegypti populations. Recently, a novel replacement V410L was identified in Brazil and its effect on vgsc was confirmed by electrophysiology. Herein, we screened V410L in 25 Ae. aegypti historical collections from Mexico, the first heterozygote appeared in 2002 and frequencies have increased in the last 16 years alongside V1,016I and F1,534C. Knowledge of the specific vgsc replacements and their interaction to confer resistance is essential to predict and to develop strategies for resistance management.
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spelling pubmed-59282502018-05-07 Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico Saavedra-Rodriguez, Karla Maloof, Farah Vera Campbell, Corey L. Garcia-Rejon, Julian Lenhart, Audrey Penilla, Patricia Rodriguez, Americo Sandoval, Arturo Acero Flores, Adriana E. Ponce, Gustavo Lozano, Saul Black, William C. Sci Rep Article Aedes aegypti is the primary urban mosquito vector of viruses causing dengue, Zika and chikungunya fevers –for which vaccines and effective pharmaceuticals are still lacking. Current strategies to suppress arbovirus outbreaks include removal of larval-breeding sites and insecticide treatment of larval and adult populations. Insecticidal control of Ae. aegypti is challenging, due to a recent rapid global increase in knockdown-resistance (kdr) to pyrethroid insecticides. Widespread, heavy use of pyrethroid space-sprays has created an immense selection pressure for kdr, which is primarily under the control of the voltage-gated sodium channel gene (vgsc). To date, eleven replacements in vgsc have been discovered, published and shown to be associated with pyrethroid resistance to varying degrees. In Mexico, F1,534C and V1,016I have co-evolved in the last 16 years across Ae. aegypti populations. Recently, a novel replacement V410L was identified in Brazil and its effect on vgsc was confirmed by electrophysiology. Herein, we screened V410L in 25 Ae. aegypti historical collections from Mexico, the first heterozygote appeared in 2002 and frequencies have increased in the last 16 years alongside V1,016I and F1,534C. Knowledge of the specific vgsc replacements and their interaction to confer resistance is essential to predict and to develop strategies for resistance management. Nature Publishing Group UK 2018-04-30 /pmc/articles/PMC5928250/ /pubmed/29712956 http://dx.doi.org/10.1038/s41598-018-25222-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Saavedra-Rodriguez, Karla
Maloof, Farah Vera
Campbell, Corey L.
Garcia-Rejon, Julian
Lenhart, Audrey
Penilla, Patricia
Rodriguez, Americo
Sandoval, Arturo Acero
Flores, Adriana E.
Ponce, Gustavo
Lozano, Saul
Black, William C.
Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico
title Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico
title_full Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico
title_fullStr Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico
title_full_unstemmed Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico
title_short Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico
title_sort parallel evolution of vgsc mutations at domains is6, iis6 and iiis6 in pyrethroid resistant aedes aegypti from mexico
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928250/
https://www.ncbi.nlm.nih.gov/pubmed/29712956
http://dx.doi.org/10.1038/s41598-018-25222-0
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