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Increased store-operated Ca(2+) entry mediated by GNB5 and STIM1
Recent human genetic studies have shown that Gβ5 is related to various clinical symptoms, such as sinus bradycardia, cognitive disability, and attention deficit hyperactivity disorder. Although the calcium signaling cascade is closely associated with a heterotrimeric G-protein, the function of Gβ5 i...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Physiological Society and The Korean Society of Pharmacology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928347/ https://www.ncbi.nlm.nih.gov/pubmed/29719456 http://dx.doi.org/10.4196/kjpp.2018.22.3.343 |
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author | Kang, Namju Kang, Jung Yun Park, Soonhong Shin, Dong Min |
author_facet | Kang, Namju Kang, Jung Yun Park, Soonhong Shin, Dong Min |
author_sort | Kang, Namju |
collection | PubMed |
description | Recent human genetic studies have shown that Gβ5 is related to various clinical symptoms, such as sinus bradycardia, cognitive disability, and attention deficit hyperactivity disorder. Although the calcium signaling cascade is closely associated with a heterotrimeric G-protein, the function of Gβ5 in calcium signaling and its relevance to clinical symptoms remain unknown. In this study, we investigated the in vitro changes of store-operated calcium entry (SOCE) with exogenous expression of Gβ5. The cells expressing Gβ5 had enhanced SOCE after depletion of calcium ion inside the endoplasmic reticulum. Gβ5 also augmented Stim1- and Orai1-dependent SOCE. An ORAI1 loss-of-function mutant did not show inhibition of Gβ5-induced SOCE, and a STIM1-ERM truncation mutant showed no enhancement of SOCE. These results suggested a novel role of GNB5 and Stim1, and provided insight into the regulatory mechanism of SOCE. |
format | Online Article Text |
id | pubmed-5928347 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Korean Physiological Society and The Korean Society of Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-59283472018-05-01 Increased store-operated Ca(2+) entry mediated by GNB5 and STIM1 Kang, Namju Kang, Jung Yun Park, Soonhong Shin, Dong Min Korean J Physiol Pharmacol Original Article Recent human genetic studies have shown that Gβ5 is related to various clinical symptoms, such as sinus bradycardia, cognitive disability, and attention deficit hyperactivity disorder. Although the calcium signaling cascade is closely associated with a heterotrimeric G-protein, the function of Gβ5 in calcium signaling and its relevance to clinical symptoms remain unknown. In this study, we investigated the in vitro changes of store-operated calcium entry (SOCE) with exogenous expression of Gβ5. The cells expressing Gβ5 had enhanced SOCE after depletion of calcium ion inside the endoplasmic reticulum. Gβ5 also augmented Stim1- and Orai1-dependent SOCE. An ORAI1 loss-of-function mutant did not show inhibition of Gβ5-induced SOCE, and a STIM1-ERM truncation mutant showed no enhancement of SOCE. These results suggested a novel role of GNB5 and Stim1, and provided insight into the regulatory mechanism of SOCE. The Korean Physiological Society and The Korean Society of Pharmacology 2018-05 2018-04-25 /pmc/articles/PMC5928347/ /pubmed/29719456 http://dx.doi.org/10.4196/kjpp.2018.22.3.343 Text en Copyright © Korean J Physiol Pharmacol http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kang, Namju Kang, Jung Yun Park, Soonhong Shin, Dong Min Increased store-operated Ca(2+) entry mediated by GNB5 and STIM1 |
title | Increased store-operated Ca(2+) entry mediated by GNB5 and STIM1 |
title_full | Increased store-operated Ca(2+) entry mediated by GNB5 and STIM1 |
title_fullStr | Increased store-operated Ca(2+) entry mediated by GNB5 and STIM1 |
title_full_unstemmed | Increased store-operated Ca(2+) entry mediated by GNB5 and STIM1 |
title_short | Increased store-operated Ca(2+) entry mediated by GNB5 and STIM1 |
title_sort | increased store-operated ca(2+) entry mediated by gnb5 and stim1 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928347/ https://www.ncbi.nlm.nih.gov/pubmed/29719456 http://dx.doi.org/10.4196/kjpp.2018.22.3.343 |
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