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MicroRNA-4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1

Increasing evidence has shown that abnormal expression of miR-4284 participates in the progression of several types of cancer. However, the expression and the role of miR-4284 in gastric cancer remain largely unknown. Therefore, in the present study the miR-4284 expression levels in gastric cancer t...

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Autores principales: Li, Yansen, Shen, Zhanlong, Jiang, Hongpeng, Lai, Zhiyong, Wang, Zhu, Jiang, Kewei, Ye, Yingjiang, Wang, Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928641/
https://www.ncbi.nlm.nih.gov/pubmed/29512746
http://dx.doi.org/10.3892/mmr.2018.8671
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author Li, Yansen
Shen, Zhanlong
Jiang, Hongpeng
Lai, Zhiyong
Wang, Zhu
Jiang, Kewei
Ye, Yingjiang
Wang, Shan
author_facet Li, Yansen
Shen, Zhanlong
Jiang, Hongpeng
Lai, Zhiyong
Wang, Zhu
Jiang, Kewei
Ye, Yingjiang
Wang, Shan
author_sort Li, Yansen
collection PubMed
description Increasing evidence has shown that abnormal expression of miR-4284 participates in the progression of several types of cancer. However, the expression and the role of miR-4284 in gastric cancer remain largely unknown. Therefore, in the present study the miR-4284 expression levels in gastric cancer tissues and cell lines, was examined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and found that miR-4284 was significantly upregulated in 40 pairs of gastric cancer tissues and five gastric cancer cell lines compared to the corresponding normal tissues and GES-1 cell line. In addition, increased miR-4284 expression was positively associated with TNM stage (P=0.035), distal metastasis (P=0.022) and poor prognosis in gastric cancer patients. Furthermore, the overexpression of miR-4284 expression was shown to promote cell proliferation, clone formation, invasion and migration, while the suppression of miR-4284 expression induced opposite effects. Additionally, luciferase reporter assay was conducted and showed that ten-eleven translocation 1 (TET1), a tumor suppressor gene that regulating cell survival and metastasis, was a direct target of miR-4284. Upregulated miR-4284 decreased the mRNA and protein levels of TET1 in SGC-7901 cells and downregulated miR-4284 increased the mRNA and protein levels of TET1 in AGS cells. In addition, miR-4284 expression was negatively correlated with the TET1 expression in gastric cancer tissues. Moreover, inhibition of TET1 suppressed the effect of miR-4284 inhibitors on cell proliferation in AGS cells. Therefore, data demonstrated that miR-4284 could promote tumor cell growth, migration and invasion by directly targeting TET1 in gastric cancer, which may provide a potential therapeutic target for gastric cancer treatment.
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spelling pubmed-59286412018-05-07 MicroRNA-4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1 Li, Yansen Shen, Zhanlong Jiang, Hongpeng Lai, Zhiyong Wang, Zhu Jiang, Kewei Ye, Yingjiang Wang, Shan Mol Med Rep Articles Increasing evidence has shown that abnormal expression of miR-4284 participates in the progression of several types of cancer. However, the expression and the role of miR-4284 in gastric cancer remain largely unknown. Therefore, in the present study the miR-4284 expression levels in gastric cancer tissues and cell lines, was examined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and found that miR-4284 was significantly upregulated in 40 pairs of gastric cancer tissues and five gastric cancer cell lines compared to the corresponding normal tissues and GES-1 cell line. In addition, increased miR-4284 expression was positively associated with TNM stage (P=0.035), distal metastasis (P=0.022) and poor prognosis in gastric cancer patients. Furthermore, the overexpression of miR-4284 expression was shown to promote cell proliferation, clone formation, invasion and migration, while the suppression of miR-4284 expression induced opposite effects. Additionally, luciferase reporter assay was conducted and showed that ten-eleven translocation 1 (TET1), a tumor suppressor gene that regulating cell survival and metastasis, was a direct target of miR-4284. Upregulated miR-4284 decreased the mRNA and protein levels of TET1 in SGC-7901 cells and downregulated miR-4284 increased the mRNA and protein levels of TET1 in AGS cells. In addition, miR-4284 expression was negatively correlated with the TET1 expression in gastric cancer tissues. Moreover, inhibition of TET1 suppressed the effect of miR-4284 inhibitors on cell proliferation in AGS cells. Therefore, data demonstrated that miR-4284 could promote tumor cell growth, migration and invasion by directly targeting TET1 in gastric cancer, which may provide a potential therapeutic target for gastric cancer treatment. D.A. Spandidos 2018-05 2018-03-01 /pmc/articles/PMC5928641/ /pubmed/29512746 http://dx.doi.org/10.3892/mmr.2018.8671 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Yansen
Shen, Zhanlong
Jiang, Hongpeng
Lai, Zhiyong
Wang, Zhu
Jiang, Kewei
Ye, Yingjiang
Wang, Shan
MicroRNA-4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1
title MicroRNA-4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1
title_full MicroRNA-4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1
title_fullStr MicroRNA-4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1
title_full_unstemmed MicroRNA-4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1
title_short MicroRNA-4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1
title_sort microrna-4284 promotes gastric cancer tumorigenicity by targeting ten-eleven translocation 1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928641/
https://www.ncbi.nlm.nih.gov/pubmed/29512746
http://dx.doi.org/10.3892/mmr.2018.8671
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