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Characterization of Invasive Salmonella Serogroup C1 Infections in Mali

Nontyphoidal Salmonella (NTS) are the leading cause of foodborne infections worldwide and a major cause of bloodstream infections in infants and HIV-infected adults in sub-Saharan Africa (SSA). Salmonella Typhimurium (serogroup B) and Salmonella Enteritidis (serogroup D) are the most common serovars...

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Autores principales: Fuche, Fabien J., Sen, Sunil, Jones, Jennifer A., Nkeze, Joseph, Permala-Booth, Jasnehta, Tapia, Milagritos D., Sow, Samba O., Tamboura, Boubou, Touré, Aliou, Onwuchekwa, Uma, Sylla, Mamadou, Kotloff, Karen L., Tennant, Sharon M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Tropical Medicine and Hygiene 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5929196/
https://www.ncbi.nlm.nih.gov/pubmed/29280425
http://dx.doi.org/10.4269/ajtmh.17-0508
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author Fuche, Fabien J.
Sen, Sunil
Jones, Jennifer A.
Nkeze, Joseph
Permala-Booth, Jasnehta
Tapia, Milagritos D.
Sow, Samba O.
Tamboura, Boubou
Touré, Aliou
Onwuchekwa, Uma
Sylla, Mamadou
Kotloff, Karen L.
Tennant, Sharon M.
author_facet Fuche, Fabien J.
Sen, Sunil
Jones, Jennifer A.
Nkeze, Joseph
Permala-Booth, Jasnehta
Tapia, Milagritos D.
Sow, Samba O.
Tamboura, Boubou
Touré, Aliou
Onwuchekwa, Uma
Sylla, Mamadou
Kotloff, Karen L.
Tennant, Sharon M.
author_sort Fuche, Fabien J.
collection PubMed
description Nontyphoidal Salmonella (NTS) are the leading cause of foodborne infections worldwide and a major cause of bloodstream infections in infants and HIV-infected adults in sub-Saharan Africa (SSA). Salmonella Typhimurium (serogroup B) and Salmonella Enteritidis (serogroup D) are the most common serovars in this region. However, data describing rarer invasive NTS serovars, particularly those belonging to serogroups C1 and C2, circulating in SSA are lacking. We previously conducted systematic blood culture surveillance on pediatric patients in Bamako, Mali, from 2002 to 2014, and the results showed that serovars Typhimurium and Enteritidis accounted for 32% and 36% of isolates, respectively. Here, we present data on 27 Salmonella serogroup C1 strains that were isolated during this previous study. The strains were typed by serum agglutination and multilocus sequence typing (MLST). Sixteen strains were Salmonella Paratyphi C, four were Salmonella Colindale, and two were Salmonella Virchow. Interestingly, five strains were identified as the very rare Salmonella Brazzaville using a combination of serum agglutination and flagellin gene typing. Phenotypic characterization showed that Salmonella Brazzaville produced biofilm and exhibited catalase activity, which were not statistically different from the gastroenteritis-associated Salmonella Typhimurium sequence type (ST) 19. All tested Salmonella Paratyphi C strains were poor biofilm producers and showed significantly less catalase activity than Salmonella Typhimurium ST19. Overall, our study provides insight into the Salmonella serogroup C1 serovars that cause invasive disease in infants in Mali. In addition, we show that MLST and flagellin gene sequencing, in association with traditional serum agglutination, are invaluable tools to help identify rare Salmonella serovars.
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spelling pubmed-59291962018-05-08 Characterization of Invasive Salmonella Serogroup C1 Infections in Mali Fuche, Fabien J. Sen, Sunil Jones, Jennifer A. Nkeze, Joseph Permala-Booth, Jasnehta Tapia, Milagritos D. Sow, Samba O. Tamboura, Boubou Touré, Aliou Onwuchekwa, Uma Sylla, Mamadou Kotloff, Karen L. Tennant, Sharon M. Am J Trop Med Hyg Articles Nontyphoidal Salmonella (NTS) are the leading cause of foodborne infections worldwide and a major cause of bloodstream infections in infants and HIV-infected adults in sub-Saharan Africa (SSA). Salmonella Typhimurium (serogroup B) and Salmonella Enteritidis (serogroup D) are the most common serovars in this region. However, data describing rarer invasive NTS serovars, particularly those belonging to serogroups C1 and C2, circulating in SSA are lacking. We previously conducted systematic blood culture surveillance on pediatric patients in Bamako, Mali, from 2002 to 2014, and the results showed that serovars Typhimurium and Enteritidis accounted for 32% and 36% of isolates, respectively. Here, we present data on 27 Salmonella serogroup C1 strains that were isolated during this previous study. The strains were typed by serum agglutination and multilocus sequence typing (MLST). Sixteen strains were Salmonella Paratyphi C, four were Salmonella Colindale, and two were Salmonella Virchow. Interestingly, five strains were identified as the very rare Salmonella Brazzaville using a combination of serum agglutination and flagellin gene typing. Phenotypic characterization showed that Salmonella Brazzaville produced biofilm and exhibited catalase activity, which were not statistically different from the gastroenteritis-associated Salmonella Typhimurium sequence type (ST) 19. All tested Salmonella Paratyphi C strains were poor biofilm producers and showed significantly less catalase activity than Salmonella Typhimurium ST19. Overall, our study provides insight into the Salmonella serogroup C1 serovars that cause invasive disease in infants in Mali. In addition, we show that MLST and flagellin gene sequencing, in association with traditional serum agglutination, are invaluable tools to help identify rare Salmonella serovars. The American Society of Tropical Medicine and Hygiene 2018-02 2017-12-26 /pmc/articles/PMC5929196/ /pubmed/29280425 http://dx.doi.org/10.4269/ajtmh.17-0508 Text en © The American Society of Tropical Medicine and Hygiene This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Articles
Fuche, Fabien J.
Sen, Sunil
Jones, Jennifer A.
Nkeze, Joseph
Permala-Booth, Jasnehta
Tapia, Milagritos D.
Sow, Samba O.
Tamboura, Boubou
Touré, Aliou
Onwuchekwa, Uma
Sylla, Mamadou
Kotloff, Karen L.
Tennant, Sharon M.
Characterization of Invasive Salmonella Serogroup C1 Infections in Mali
title Characterization of Invasive Salmonella Serogroup C1 Infections in Mali
title_full Characterization of Invasive Salmonella Serogroup C1 Infections in Mali
title_fullStr Characterization of Invasive Salmonella Serogroup C1 Infections in Mali
title_full_unstemmed Characterization of Invasive Salmonella Serogroup C1 Infections in Mali
title_short Characterization of Invasive Salmonella Serogroup C1 Infections in Mali
title_sort characterization of invasive salmonella serogroup c1 infections in mali
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5929196/
https://www.ncbi.nlm.nih.gov/pubmed/29280425
http://dx.doi.org/10.4269/ajtmh.17-0508
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