Sample manipulation and data assembly for robust microcrystal synchrotron crystallography

With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo­graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for ro...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Gongrui, Fuchs, Martin R., Shi, Wuxian, Skinner, John, Berman, Evanna, Ogata, Craig M., Hendrickson, Wayne A., McSweeney, Sean, Liu, Qun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2018
Materias:
Research Letters
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5929371/
https://www.ncbi.nlm.nih.gov/pubmed/29755741
http://dx.doi.org/10.1107/S2052252518005389
_version_ 1783319391708905472
author Guo, Gongrui
Fuchs, Martin R.
Shi, Wuxian
Skinner, John
Berman, Evanna
Ogata, Craig M.
Hendrickson, Wayne A.
McSweeney, Sean
Liu, Qun
author_facet Guo, Gongrui
Fuchs, Martin R.
Shi, Wuxian
Skinner, John
Berman, Evanna
Ogata, Craig M.
Hendrickson, Wayne A.
McSweeney, Sean
Liu, Qun
author_sort Guo, Gongrui
collection PubMed
description With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo­graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.
format Online
Article
Text
id pubmed-5929371
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher International Union of Crystallography
record_format MEDLINE/PubMed
spelling pubmed-59293712018-05-11 Sample manipulation and data assembly for robust microcrystal synchrotron crystallography Guo, Gongrui Fuchs, Martin R. Shi, Wuxian Skinner, John Berman, Evanna Ogata, Craig M. Hendrickson, Wayne A. McSweeney, Sean Liu, Qun IUCrJ Research Letters With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo­graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines. International Union of Crystallography 2018-04-19 /pmc/articles/PMC5929371/ /pubmed/29755741 http://dx.doi.org/10.1107/S2052252518005389 Text en © Gongrui Guo et al. 2018 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Research Letters
Guo, Gongrui
Fuchs, Martin R.
Shi, Wuxian
Skinner, John
Berman, Evanna
Ogata, Craig M.
Hendrickson, Wayne A.
McSweeney, Sean
Liu, Qun
Sample manipulation and data assembly for robust microcrystal synchrotron crystallography
title Sample manipulation and data assembly for robust microcrystal synchrotron crystallography
title_full Sample manipulation and data assembly for robust microcrystal synchrotron crystallography
title_fullStr Sample manipulation and data assembly for robust microcrystal synchrotron crystallography
title_full_unstemmed Sample manipulation and data assembly for robust microcrystal synchrotron crystallography
title_short Sample manipulation and data assembly for robust microcrystal synchrotron crystallography
title_sort sample manipulation and data assembly for robust microcrystal synchrotron crystallography
topic Research Letters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5929371/
https://www.ncbi.nlm.nih.gov/pubmed/29755741
http://dx.doi.org/10.1107/S2052252518005389
work_keys_str_mv AT guogongrui samplemanipulationanddataassemblyforrobustmicrocrystalsynchrotroncrystallography
AT fuchsmartinr samplemanipulationanddataassemblyforrobustmicrocrystalsynchrotroncrystallography
AT shiwuxian samplemanipulationanddataassemblyforrobustmicrocrystalsynchrotroncrystallography
AT skinnerjohn samplemanipulationanddataassemblyforrobustmicrocrystalsynchrotroncrystallography
AT bermanevanna samplemanipulationanddataassemblyforrobustmicrocrystalsynchrotroncrystallography
AT ogatacraigm samplemanipulationanddataassemblyforrobustmicrocrystalsynchrotroncrystallography
AT hendricksonwaynea samplemanipulationanddataassemblyforrobustmicrocrystalsynchrotroncrystallography
AT mcsweeneysean samplemanipulationanddataassemblyforrobustmicrocrystalsynchrotroncrystallography
AT liuqun samplemanipulationanddataassemblyforrobustmicrocrystalsynchrotroncrystallography

Ejemplares similares