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Comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells
A novel biological model was created for the comparison of grapevine embryogenic cells (EC) and nonembryogenic cells (NEC) sharing a common genetic background but distinct phenotypes, when cultured on their respective most appropriate media. Cytological characterization, (1)H‐NMR analysis of intrace...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5929931/ https://www.ncbi.nlm.nih.gov/pubmed/29744293 http://dx.doi.org/10.1002/2211-5463.12415 |
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author | Parrilla, Jonathan Gaillard, Cécile Verbeke, Jérémy Maucourt, Mickaël Aleksandrov, Radoslav A. Thibault, Florence Fleurat‐Lessard, Pierrette Gibon, Yves Rolin, Dominique Atanassova, Rossitza |
author_facet | Parrilla, Jonathan Gaillard, Cécile Verbeke, Jérémy Maucourt, Mickaël Aleksandrov, Radoslav A. Thibault, Florence Fleurat‐Lessard, Pierrette Gibon, Yves Rolin, Dominique Atanassova, Rossitza |
author_sort | Parrilla, Jonathan |
collection | PubMed |
description | A novel biological model was created for the comparison of grapevine embryogenic cells (EC) and nonembryogenic cells (NEC) sharing a common genetic background but distinct phenotypes, when cultured on their respective most appropriate media. Cytological characterization, (1)H‐NMR analysis of intracellular metabolites, and glycolytic enzyme activities provided evidence for the marked metabolic differences between EC and NEC. The EC were characterized by a moderate and organized cell proliferation, coupled with a low flux through glycolysis, high capacity of phosphoenolpyruvate carboxylase and glucokinase, and high oxygen consumption. The NEC displayed strong anarchic growth, and their high rate of glycolysis due to the low energetic efficiency of the fermentative metabolism is confirmed by increased enolase capacity and low oxygen consumption. |
format | Online Article Text |
id | pubmed-5929931 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59299312018-05-09 Comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells Parrilla, Jonathan Gaillard, Cécile Verbeke, Jérémy Maucourt, Mickaël Aleksandrov, Radoslav A. Thibault, Florence Fleurat‐Lessard, Pierrette Gibon, Yves Rolin, Dominique Atanassova, Rossitza FEBS Open Bio Research Articles A novel biological model was created for the comparison of grapevine embryogenic cells (EC) and nonembryogenic cells (NEC) sharing a common genetic background but distinct phenotypes, when cultured on their respective most appropriate media. Cytological characterization, (1)H‐NMR analysis of intracellular metabolites, and glycolytic enzyme activities provided evidence for the marked metabolic differences between EC and NEC. The EC were characterized by a moderate and organized cell proliferation, coupled with a low flux through glycolysis, high capacity of phosphoenolpyruvate carboxylase and glucokinase, and high oxygen consumption. The NEC displayed strong anarchic growth, and their high rate of glycolysis due to the low energetic efficiency of the fermentative metabolism is confirmed by increased enolase capacity and low oxygen consumption. John Wiley and Sons Inc. 2018-04-17 /pmc/articles/PMC5929931/ /pubmed/29744293 http://dx.doi.org/10.1002/2211-5463.12415 Text en © 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Parrilla, Jonathan Gaillard, Cécile Verbeke, Jérémy Maucourt, Mickaël Aleksandrov, Radoslav A. Thibault, Florence Fleurat‐Lessard, Pierrette Gibon, Yves Rolin, Dominique Atanassova, Rossitza Comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells |
title | Comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells |
title_full | Comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells |
title_fullStr | Comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells |
title_full_unstemmed | Comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells |
title_short | Comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells |
title_sort | comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5929931/ https://www.ncbi.nlm.nih.gov/pubmed/29744293 http://dx.doi.org/10.1002/2211-5463.12415 |
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