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Isolation of native plasma membrane H(+)‐ATPase (Pma1p) in both the active and basal activation states
The yeast plasma membrane H(+)‐ATPase Pma1p is a P‐type ATPase that energizes the yeast plasma membrane. Pma1p exists in two activation states: an autoinhibited basal state and an activated state. Here we show that functional and stable Pma1p can be purified in native form and reconstituted in artif...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5929935/ https://www.ncbi.nlm.nih.gov/pubmed/29744292 http://dx.doi.org/10.1002/2211-5463.12413 |