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Pathology of natural Francisella tularensis subsp. holarctica infection in two yellow-necked mice (Apodemus flavicollis)

BACKGROUND: Tularemia is a zoonosis caused by the bacterium Francisella tularensis. It has a wide host range, which includes mammals, birds and invertebrates. F. tularensis has often been isolated from various species of small rodents, but the pathology in naturally infected wild rodent species has...

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Autores principales: Hestvik, Gete, Uhlhorn, Henrik, Mattsson, Roland, Westergren, Eva, Södersten, Fredrik, Åkerström, Sara, Gavier-Widén, Dolores
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5930785/
https://www.ncbi.nlm.nih.gov/pubmed/29716621
http://dx.doi.org/10.1186/s13028-018-0381-9
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author Hestvik, Gete
Uhlhorn, Henrik
Mattsson, Roland
Westergren, Eva
Södersten, Fredrik
Åkerström, Sara
Gavier-Widén, Dolores
author_facet Hestvik, Gete
Uhlhorn, Henrik
Mattsson, Roland
Westergren, Eva
Södersten, Fredrik
Åkerström, Sara
Gavier-Widén, Dolores
author_sort Hestvik, Gete
collection PubMed
description BACKGROUND: Tularemia is a zoonosis caused by the bacterium Francisella tularensis. It has a wide host range, which includes mammals, birds and invertebrates. F. tularensis has often been isolated from various species of small rodents, but the pathology in naturally infected wild rodent species has rarely been reported. CASE PRESENTATION: Herein, we describe the pathology of tularemia in two naturally infected wild yellow-necked mice (Apodemus flavicollis). To visualize F. tularensis subsp. holarctica, indirect immunofluorescence and immunohistochemistry were applied on tissue sections. Real time polymerase chain reaction detected the bacterium in samples from liver and spleen in both mice. The only finding at necropsy was splenomegaly in one of the mice. Histological examination revealed necrotic foci in the liver associated with mild inflammation in both mice. Immunohistochemistry and indirect immunofluorescence showed bacteria disseminated in many organs, in the cytoplasm of macrophages, and intravascularly. CONCLUSIONS: The two yellow-necked mice died of an acute disease caused by tularemic infection disseminated to many organs. Further investigations of naturally infected small rodents are important to better understand the variability in pathological presentation caused by infection by F. tularensis subsp. holarctica, as well to elucidate the importance of small rodents as transmitters and/or reservoirs.
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spelling pubmed-59307852018-05-09 Pathology of natural Francisella tularensis subsp. holarctica infection in two yellow-necked mice (Apodemus flavicollis) Hestvik, Gete Uhlhorn, Henrik Mattsson, Roland Westergren, Eva Södersten, Fredrik Åkerström, Sara Gavier-Widén, Dolores Acta Vet Scand Case Report BACKGROUND: Tularemia is a zoonosis caused by the bacterium Francisella tularensis. It has a wide host range, which includes mammals, birds and invertebrates. F. tularensis has often been isolated from various species of small rodents, but the pathology in naturally infected wild rodent species has rarely been reported. CASE PRESENTATION: Herein, we describe the pathology of tularemia in two naturally infected wild yellow-necked mice (Apodemus flavicollis). To visualize F. tularensis subsp. holarctica, indirect immunofluorescence and immunohistochemistry were applied on tissue sections. Real time polymerase chain reaction detected the bacterium in samples from liver and spleen in both mice. The only finding at necropsy was splenomegaly in one of the mice. Histological examination revealed necrotic foci in the liver associated with mild inflammation in both mice. Immunohistochemistry and indirect immunofluorescence showed bacteria disseminated in many organs, in the cytoplasm of macrophages, and intravascularly. CONCLUSIONS: The two yellow-necked mice died of an acute disease caused by tularemic infection disseminated to many organs. Further investigations of naturally infected small rodents are important to better understand the variability in pathological presentation caused by infection by F. tularensis subsp. holarctica, as well to elucidate the importance of small rodents as transmitters and/or reservoirs. BioMed Central 2018-05-02 /pmc/articles/PMC5930785/ /pubmed/29716621 http://dx.doi.org/10.1186/s13028-018-0381-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Case Report
Hestvik, Gete
Uhlhorn, Henrik
Mattsson, Roland
Westergren, Eva
Södersten, Fredrik
Åkerström, Sara
Gavier-Widén, Dolores
Pathology of natural Francisella tularensis subsp. holarctica infection in two yellow-necked mice (Apodemus flavicollis)
title Pathology of natural Francisella tularensis subsp. holarctica infection in two yellow-necked mice (Apodemus flavicollis)
title_full Pathology of natural Francisella tularensis subsp. holarctica infection in two yellow-necked mice (Apodemus flavicollis)
title_fullStr Pathology of natural Francisella tularensis subsp. holarctica infection in two yellow-necked mice (Apodemus flavicollis)
title_full_unstemmed Pathology of natural Francisella tularensis subsp. holarctica infection in two yellow-necked mice (Apodemus flavicollis)
title_short Pathology of natural Francisella tularensis subsp. holarctica infection in two yellow-necked mice (Apodemus flavicollis)
title_sort pathology of natural francisella tularensis subsp. holarctica infection in two yellow-necked mice (apodemus flavicollis)
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5930785/
https://www.ncbi.nlm.nih.gov/pubmed/29716621
http://dx.doi.org/10.1186/s13028-018-0381-9
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