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Comparison of McMaster and FECPAK(G2) methods for counting nematode eggs in the faeces of alpacas

This study aimed to compare the FECPAK(G2) and the McMaster techniques for counting of gastrointestinal nematode eggs in the faeces of alpacas using two floatation solutions (saturated sodium chloride and sucrose solutions). Faecal eggs counts from both techniques were compared using the Lin’s conco...

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Detalles Bibliográficos
Autores principales: Rashid, Mohammed H., Stevenson, Mark A., Waenga, Shea, Mirams, Greg, Campbell, Angus J. D., Vaughan, Jane L., Jabbar, Abdul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5930814/
https://www.ncbi.nlm.nih.gov/pubmed/29716657
http://dx.doi.org/10.1186/s13071-018-2861-1
Descripción
Sumario:This study aimed to compare the FECPAK(G2) and the McMaster techniques for counting of gastrointestinal nematode eggs in the faeces of alpacas using two floatation solutions (saturated sodium chloride and sucrose solutions). Faecal eggs counts from both techniques were compared using the Lin’s concordance correlation coefficient and Bland and Altman statistics. Results showed moderate to good agreement between the two methods, with better agreement achieved when saturated sugar is used as a floatation fluid, particularly when faecal egg counts are less than 1000 eggs per gram of faeces. To the best of our knowledge this is the first study to assess agreement of measurements between McMaster and FECPAK(G2) methods for estimating faecal eggs in South American camelids. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-2861-1) contains supplementary material, which is available to authorized users.