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Comparison of McMaster and FECPAK(G2) methods for counting nematode eggs in the faeces of alpacas

This study aimed to compare the FECPAK(G2) and the McMaster techniques for counting of gastrointestinal nematode eggs in the faeces of alpacas using two floatation solutions (saturated sodium chloride and sucrose solutions). Faecal eggs counts from both techniques were compared using the Lin’s conco...

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Autores principales: Rashid, Mohammed H., Stevenson, Mark A., Waenga, Shea, Mirams, Greg, Campbell, Angus J. D., Vaughan, Jane L., Jabbar, Abdul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5930814/
https://www.ncbi.nlm.nih.gov/pubmed/29716657
http://dx.doi.org/10.1186/s13071-018-2861-1
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author Rashid, Mohammed H.
Stevenson, Mark A.
Waenga, Shea
Mirams, Greg
Campbell, Angus J. D.
Vaughan, Jane L.
Jabbar, Abdul
author_facet Rashid, Mohammed H.
Stevenson, Mark A.
Waenga, Shea
Mirams, Greg
Campbell, Angus J. D.
Vaughan, Jane L.
Jabbar, Abdul
author_sort Rashid, Mohammed H.
collection PubMed
description This study aimed to compare the FECPAK(G2) and the McMaster techniques for counting of gastrointestinal nematode eggs in the faeces of alpacas using two floatation solutions (saturated sodium chloride and sucrose solutions). Faecal eggs counts from both techniques were compared using the Lin’s concordance correlation coefficient and Bland and Altman statistics. Results showed moderate to good agreement between the two methods, with better agreement achieved when saturated sugar is used as a floatation fluid, particularly when faecal egg counts are less than 1000 eggs per gram of faeces. To the best of our knowledge this is the first study to assess agreement of measurements between McMaster and FECPAK(G2) methods for estimating faecal eggs in South American camelids. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-2861-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-59308142018-05-09 Comparison of McMaster and FECPAK(G2) methods for counting nematode eggs in the faeces of alpacas Rashid, Mohammed H. Stevenson, Mark A. Waenga, Shea Mirams, Greg Campbell, Angus J. D. Vaughan, Jane L. Jabbar, Abdul Parasit Vectors Letter to the Editor This study aimed to compare the FECPAK(G2) and the McMaster techniques for counting of gastrointestinal nematode eggs in the faeces of alpacas using two floatation solutions (saturated sodium chloride and sucrose solutions). Faecal eggs counts from both techniques were compared using the Lin’s concordance correlation coefficient and Bland and Altman statistics. Results showed moderate to good agreement between the two methods, with better agreement achieved when saturated sugar is used as a floatation fluid, particularly when faecal egg counts are less than 1000 eggs per gram of faeces. To the best of our knowledge this is the first study to assess agreement of measurements between McMaster and FECPAK(G2) methods for estimating faecal eggs in South American camelids. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-2861-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-02 /pmc/articles/PMC5930814/ /pubmed/29716657 http://dx.doi.org/10.1186/s13071-018-2861-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Letter to the Editor
Rashid, Mohammed H.
Stevenson, Mark A.
Waenga, Shea
Mirams, Greg
Campbell, Angus J. D.
Vaughan, Jane L.
Jabbar, Abdul
Comparison of McMaster and FECPAK(G2) methods for counting nematode eggs in the faeces of alpacas
title Comparison of McMaster and FECPAK(G2) methods for counting nematode eggs in the faeces of alpacas
title_full Comparison of McMaster and FECPAK(G2) methods for counting nematode eggs in the faeces of alpacas
title_fullStr Comparison of McMaster and FECPAK(G2) methods for counting nematode eggs in the faeces of alpacas
title_full_unstemmed Comparison of McMaster and FECPAK(G2) methods for counting nematode eggs in the faeces of alpacas
title_short Comparison of McMaster and FECPAK(G2) methods for counting nematode eggs in the faeces of alpacas
title_sort comparison of mcmaster and fecpak(g2) methods for counting nematode eggs in the faeces of alpacas
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5930814/
https://www.ncbi.nlm.nih.gov/pubmed/29716657
http://dx.doi.org/10.1186/s13071-018-2861-1
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