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Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

A normal adult heart is composed of several different cell types, among which cardiac mesenchymal stromal cells represent an abundant population. The isolation of these cells offers the possibility of studying their involvement in cardiac diseases, and, in addition, provides a useful primary cell mo...

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Autores principales: Pilato, Chiara Assunta, Stadiotti, Ilaria, Maione, Angela Serena, Saverio, Valentina, Catto, Valentina, Tundo, Fabrizio, Dello Russo, Antonio, Tondo, Claudio, Pompilio, Giulio, Casella, Michela, Sommariva, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931414/
https://www.ncbi.nlm.nih.gov/pubmed/29553539
http://dx.doi.org/10.3791/57263
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author Pilato, Chiara Assunta
Stadiotti, Ilaria
Maione, Angela Serena
Saverio, Valentina
Catto, Valentina
Tundo, Fabrizio
Dello Russo, Antonio
Tondo, Claudio
Pompilio, Giulio
Casella, Michela
Sommariva, Elena
author_facet Pilato, Chiara Assunta
Stadiotti, Ilaria
Maione, Angela Serena
Saverio, Valentina
Catto, Valentina
Tundo, Fabrizio
Dello Russo, Antonio
Tondo, Claudio
Pompilio, Giulio
Casella, Michela
Sommariva, Elena
author_sort Pilato, Chiara Assunta
collection PubMed
description A normal adult heart is composed of several different cell types, among which cardiac mesenchymal stromal cells represent an abundant population. The isolation of these cells offers the possibility of studying their involvement in cardiac diseases, and, in addition, provides a useful primary cell model to investigate biological mechanisms. Here, the method for the isolation of C-MSC from arrhythmogenic cardiomyopathy patients' bioptic samples is described. The endomyocardial biopsy sampling is guided in the right ventricular areas adjacent to the scar visualized by electro-anatomical mapping. The digestion of the biopsies in collagenase and their plating on a plastic dish in culture medium to allow C-MSC growth is described. The isolated cells can be expanded in culture for several passages. To confirm their mesenchymal phenotype, the description of immuno-phenotypical characterization is provided. C-MSC are able to differentiate into several cell types like adipocytes, chondrocytes, and osteoblasts: in the context of ACM, characterized by adipocyte deposits in patients' hearts, the protocols for the adipogenic differentiation of C-MSC and the characterization of lipid droplet accumulation are described.
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spelling pubmed-59314142018-05-16 Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients Pilato, Chiara Assunta Stadiotti, Ilaria Maione, Angela Serena Saverio, Valentina Catto, Valentina Tundo, Fabrizio Dello Russo, Antonio Tondo, Claudio Pompilio, Giulio Casella, Michela Sommariva, Elena J Vis Exp Developmental Biology A normal adult heart is composed of several different cell types, among which cardiac mesenchymal stromal cells represent an abundant population. The isolation of these cells offers the possibility of studying their involvement in cardiac diseases, and, in addition, provides a useful primary cell model to investigate biological mechanisms. Here, the method for the isolation of C-MSC from arrhythmogenic cardiomyopathy patients' bioptic samples is described. The endomyocardial biopsy sampling is guided in the right ventricular areas adjacent to the scar visualized by electro-anatomical mapping. The digestion of the biopsies in collagenase and their plating on a plastic dish in culture medium to allow C-MSC growth is described. The isolated cells can be expanded in culture for several passages. To confirm their mesenchymal phenotype, the description of immuno-phenotypical characterization is provided. C-MSC are able to differentiate into several cell types like adipocytes, chondrocytes, and osteoblasts: in the context of ACM, characterized by adipocyte deposits in patients' hearts, the protocols for the adipogenic differentiation of C-MSC and the characterization of lipid droplet accumulation are described. MyJove Corporation 2018-02-28 /pmc/articles/PMC5931414/ /pubmed/29553539 http://dx.doi.org/10.3791/57263 Text en Copyright © 2018, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Developmental Biology
Pilato, Chiara Assunta
Stadiotti, Ilaria
Maione, Angela Serena
Saverio, Valentina
Catto, Valentina
Tundo, Fabrizio
Dello Russo, Antonio
Tondo, Claudio
Pompilio, Giulio
Casella, Michela
Sommariva, Elena
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
title Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
title_full Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
title_fullStr Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
title_full_unstemmed Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
title_short Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
title_sort isolation and characterization of cardiac mesenchymal stromal cells from endomyocardial bioptic samples of arrhythmogenic cardiomyopathy patients
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931414/
https://www.ncbi.nlm.nih.gov/pubmed/29553539
http://dx.doi.org/10.3791/57263
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