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Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows
Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931544/ https://www.ncbi.nlm.nih.gov/pubmed/29717158 http://dx.doi.org/10.1038/s41598-018-24896-w |
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author | da Silva Duarte, Vinícius Dias, Roberto Sousa Kropinski, Andrew M. Campanaro, Stefano Treu, Laura Siqueira, Carolina Vieira, Marcella Silva da Silva Paes, Isabela Santana, Gabriele Rocha Martins, Franciele Crispim, Josicelli Souza da Silva Xavier, André Ferro, Camila Geovana Vidigal, Pedro M. P. da Silva, Cynthia Canêdo de Paula, Sérgio Oliveira |
author_facet | da Silva Duarte, Vinícius Dias, Roberto Sousa Kropinski, Andrew M. Campanaro, Stefano Treu, Laura Siqueira, Carolina Vieira, Marcella Silva da Silva Paes, Isabela Santana, Gabriele Rocha Martins, Franciele Crispim, Josicelli Souza da Silva Xavier, André Ferro, Camila Geovana Vidigal, Pedro M. P. da Silva, Cynthia Canêdo de Paula, Sérgio Oliveira |
author_sort | da Silva Duarte, Vinícius |
collection | PubMed |
description | Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present as an efficient treatment or prophylactic option. However, this makes it essential that its genetic structure, stability and interaction with the host immune system be thoroughly characterized. The present study analyzed a novel, broad host-range anti-mastitis agent, the T4virus vB_EcoM-UFV13 in genomic terms, and its activity against a MPEC strain in an experimental E. coli-induced mastitis mouse model. 4,975 Single Nucleotide Polymorphisms (SNPs) were assigned between vB_EcoM-UFV13 and E. coli phage T4 genomes with high impact on coding sequences (CDS) (37.60%) for virion proteins. Phylogenetic trees and genome analysis supported a recent infection mix between vB_EcoM-UFV13 and Shigella phage Shfl2. After a viral stability evaluation (e.g pH and temperature), intramammary administration (MOI 10) resulted in a 10-fold reduction in bacterial load. Furthermore, pro-inflammatory cytokines, such as IL-6 and TNF-α, were observed after viral treatment. This work brings the whole characterization and immune response to vB_EcoM-UFV13, a biocontrol candidate for bovine mastitis. |
format | Online Article Text |
id | pubmed-5931544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59315442018-08-29 Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows da Silva Duarte, Vinícius Dias, Roberto Sousa Kropinski, Andrew M. Campanaro, Stefano Treu, Laura Siqueira, Carolina Vieira, Marcella Silva da Silva Paes, Isabela Santana, Gabriele Rocha Martins, Franciele Crispim, Josicelli Souza da Silva Xavier, André Ferro, Camila Geovana Vidigal, Pedro M. P. da Silva, Cynthia Canêdo de Paula, Sérgio Oliveira Sci Rep Article Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present as an efficient treatment or prophylactic option. However, this makes it essential that its genetic structure, stability and interaction with the host immune system be thoroughly characterized. The present study analyzed a novel, broad host-range anti-mastitis agent, the T4virus vB_EcoM-UFV13 in genomic terms, and its activity against a MPEC strain in an experimental E. coli-induced mastitis mouse model. 4,975 Single Nucleotide Polymorphisms (SNPs) were assigned between vB_EcoM-UFV13 and E. coli phage T4 genomes with high impact on coding sequences (CDS) (37.60%) for virion proteins. Phylogenetic trees and genome analysis supported a recent infection mix between vB_EcoM-UFV13 and Shigella phage Shfl2. After a viral stability evaluation (e.g pH and temperature), intramammary administration (MOI 10) resulted in a 10-fold reduction in bacterial load. Furthermore, pro-inflammatory cytokines, such as IL-6 and TNF-α, were observed after viral treatment. This work brings the whole characterization and immune response to vB_EcoM-UFV13, a biocontrol candidate for bovine mastitis. Nature Publishing Group UK 2018-05-01 /pmc/articles/PMC5931544/ /pubmed/29717158 http://dx.doi.org/10.1038/s41598-018-24896-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article da Silva Duarte, Vinícius Dias, Roberto Sousa Kropinski, Andrew M. Campanaro, Stefano Treu, Laura Siqueira, Carolina Vieira, Marcella Silva da Silva Paes, Isabela Santana, Gabriele Rocha Martins, Franciele Crispim, Josicelli Souza da Silva Xavier, André Ferro, Camila Geovana Vidigal, Pedro M. P. da Silva, Cynthia Canêdo de Paula, Sérgio Oliveira Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
title | Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
title_full | Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
title_fullStr | Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
title_full_unstemmed | Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
title_short | Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows |
title_sort | genomic analysis and immune response in a murine mastitis model of vb_ecom-ufv13, a potential biocontrol agent for use in dairy cows |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931544/ https://www.ncbi.nlm.nih.gov/pubmed/29717158 http://dx.doi.org/10.1038/s41598-018-24896-w |
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