Cargando…

Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts

Endoreduplication, the replication of a cell's nuclear genome without subsequent cytokinesis, yields cells with increased DNA content and is associated with specialization, development and increase in cellular size. In plants, endoreduplication seems to facilitate the growth and expansion of ce...

Descripción completa

Detalles Bibliográficos
Autores principales:	Laimbeer, F. Parker E., Makris, Melissa, Veilleux, Richard E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2018
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931677/ https://www.ncbi.nlm.nih.gov/pubmed/29578518 http://dx.doi.org/10.3791/57134

Ejemplares similares

Protoplast isolation prior to flow cytometry reveals clear patterns of endoreduplication in potato tubers, related species, and some starchy root crops
por: Laimbeer, F. Parker E., et al.
Publicado: (2017)

Oral Cancer Genesis and Progression: DNA Near-Diploid Aneuploidization and Endoreduplication by High Resolution Flow Cytometry
por: Donadini, Alessandra, et al.
Publicado: (2010)

Functional Characterization and Phenotyping of Protoplasts on a Microfluidics-Based Flow Cytometry
por: Dai, Xingda, et al.
Publicado: (2022)

Development of a rapid and efficient protoplast isolation and transfection method for chickpea (Cicer arietinum)
por: Cheng, Ninghui, et al.
Publicado: (2020)

Genome diversity of tuber-bearing Solanum uncovers complex evolutionary history and targets of domestication in the cultivated potato
por: Hardigan, Michael A., et al.
Publicado: (2017)

High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry
por: Williams, Thomas, et al.
Publicado: (2018)

Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
por: Epshtein, Alona, et al.
Publicado: (2017)

An optimized permeabilization step for flow cytometry analysis of nuclear proteins in myeloid differentiation of blood cells into neutrophils
por: Viryasova, G.M., et al.
Publicado: (2019)

Viscoelastic properties of suspended cells measured with shear flow deformation cytometry
por: Gerum, Richard, et al.
Publicado: (2022)

Optimized protocol for mouse footpad immune cell isolation for single-cell RNA sequencing and flow cytometry
por: Hameed, Muddassar, et al.
Publicado: (2023)

Purification of retinal ganglion cells using low-pressure flow cytometry
por: Mcloughlin, Kiran J., et al.
Publicado: (2023)

Endoreduplication of the mouse genome in the absence of ORC1
por: Okano-Uchida, Takayuki, et al.
Publicado: (2018)

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
por: Koshy, Shyny, et al.
Publicado: (2010)

Mammalian Cells Undergo Endoreduplication in Response to Lactic Acidosis
por: Tan, Zhihao, et al.
Publicado: (2018)

Myc Prevents Apoptosis and Enhances Endoreduplication Induced by Paclitaxel
por: Gatti, Giuliana, et al.
Publicado: (2009)

Protocol for functional characterization of endoreduplicated murine trophoblast cells
por: Basak, Trishita, et al.
Publicado: (2022)

Full Spectrum Flow Cytometry as a Powerful Technology for Cancer Immunotherapy Research
por: Bonilla, Diana L., et al.
Publicado: (2021)

Cortical tension overrides geometrical cues to orient microtubules in confined protoplasts
por: Colin, Leia, et al.
Publicado: (2020)

Detection of micrometastases by flow cytometry in sentinel lymph nodes from patients with renal tumours
por: Hartana, Ciputra Adijaya, et al.
Publicado: (2016)

Re-examination of the role of endoreduplication on cell-size control in leaves
por: Tsukaya, Hirokazu
Publicado: (2019)

Endoreduplication as a part of flower ontogeny in Trifolium pratense cultivars
por: Kocová, Valéria, et al.
Publicado: (2016)

Evolutionary relationship of fat body endoreduplication and queen fecundity in termites
por: Nozaki, Tomonari, et al.
Publicado: (2019)

Cell size matters: CDKG2 regulates endoreduplication in Arabidopsis
por: Hendrix, Sophie
Publicado: (2022)

Centrosome/Cell Cycle Uncoupling and Elimination in the Endoreduplicating Intestinal Cells of C. elegans
por: Lu, Yu, et al.
Publicado: (2014)

Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose
por: Kang, Hyun Hee, et al.
Publicado: (2020)

Extracellular Vesicle Flow Cytometry Analysis and Standardization
por: Welsh, Joshua A., et al.
Publicado: (2017)

The induction of endoreduplication and polyploidy by elevated expression of 14-3-3γ
por: Gomes, Cecil J., et al.
Publicado: (2017)

Extensive nuclear reprogramming and endoreduplication in mature leaf during floral induction
por: Del Prete, Stefania, et al.
Publicado: (2019)

THE LARGE-SCALE ISOLATION OF PROTOPLASTS FROM IMMATURE TOMATO FRUIT
por: Gregory, D. W., et al.
Publicado: (1965)

Detection of cell-free histones in the cerebrospinal fluid of pediatric central nervous system malignancies by imaging flow cytometry
por: Buzova, Diana, et al.
Publicado: (2023)

Measurement of Soluble Biomarkers by Flow Cytometry
por: Antal-Szalmás, Péter, et al.
Publicado: (2013)

Characterization of the F Locus Responsible for Floral Anthocyanin Production in Potato
por: Laimbeer, F. Parker E., et al.
Publicado: (2020)

Optimization of isolation and transfection conditions of maize endosperm protoplasts
por: Hu, Yufeng, et al.
Publicado: (2020)

LMI1 homeodomain protein regulates organ proportions by spatial modulation of endoreduplication
por: Vuolo, Francesco, et al.
Publicado: (2018)

The boundary of the meristematic and elongation zones in roots: endoreduplication precedes rapid cell expansion
por: Hayashi, Kohma, et al.
Publicado: (2013)

Endoreduplication is not involved in bundle-sheath formation in the C(4) species Cleome gynandra
por: Aubry, Sylvain, et al.
Publicado: (2014)

DNA tape measurements of AraC
por: Rodgers, Michael E., et al.
Publicado: (2008)

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
por: Hansen, Louise L., et al.
Publicado: (2016)

Speciation with gene flow via cycles of isolation and migration: insights from multiple mangrove taxa
por: He, Ziwen, et al.
Publicado: (2019)

Isolation of Lineage Specific Nuclei Based on Distinct Endoreduplication Levels and Tissue-Specific Markers to Study Chromatin Accessibility Landscapes
por: Karaaslan, Ezgi Süheyla, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_i6d1j17ssqtsgr6j3l7g99eejh