Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

The purpose of this protocol is to characterize the effect of gene knockdown on presynaptic function within intact neuronal circuits. We describe a workflow on how to combine artificial microRNA (miR)-mediated RNA interference with optogenetics to achieve selective stimulation of manipulated presyna...

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Detalles Bibliográficos
Autores principales: Thalhammer, Agnes, Jaudon, Fanny, Cingolani, Lorenzo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931759/
https://www.ncbi.nlm.nih.gov/pubmed/29608168
http://dx.doi.org/10.3791/57223

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931759/
https://www.ncbi.nlm.nih.gov/pubmed/29608168
http://dx.doi.org/10.3791/57223

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