A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system

In this study, we identified a chicken liver cell line (LMH) which could strongly support the replication of ALV-J (Subgroup J of avian leukosis virus) with high viral titer. Notably, ALV-J was efficiently detected by ELISA in LMH cells 1 day before DF1 cells. In comparison with DF1 cells, LMH cells...

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Autores principales: Li, Tuofan, Xie, Jing, Lv, Lu, Sun, Shu, Dong, Xiaomei, Xie, Quan, Liang, Guangcheng, Xia, Chichao, Shao, Hongxia, Qin, Aijian, Ye, Jianqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932828/
https://www.ncbi.nlm.nih.gov/pubmed/29720272
http://dx.doi.org/10.1186/s13567-018-0537-7
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author Li, Tuofan
Xie, Jing
Lv, Lu
Sun, Shu
Dong, Xiaomei
Xie, Quan
Liang, Guangcheng
Xia, Chichao
Shao, Hongxia
Qin, Aijian
Ye, Jianqiang
author_facet Li, Tuofan
Xie, Jing
Lv, Lu
Sun, Shu
Dong, Xiaomei
Xie, Quan
Liang, Guangcheng
Xia, Chichao
Shao, Hongxia
Qin, Aijian
Ye, Jianqiang
author_sort Li, Tuofan
collection PubMed
description In this study, we identified a chicken liver cell line (LMH) which could strongly support the replication of ALV-J (Subgroup J of avian leukosis virus) with high viral titer. Notably, ALV-J was efficiently detected by ELISA in LMH cells 1 day before DF1 cells. In comparison with DF1 cells, LMH cells not only expressed higher levels of ALV-J receptor chNHE-1, but also possessed a more efficient protein expression system for foreign genes. Thus, LMH cells could be a novel tool to shorten the ALV-J eradication approach and accelerate studies on the pathogenesis and oncogenesis of ALV-J.
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spelling pubmed-59328282018-05-09 A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system Li, Tuofan Xie, Jing Lv, Lu Sun, Shu Dong, Xiaomei Xie, Quan Liang, Guangcheng Xia, Chichao Shao, Hongxia Qin, Aijian Ye, Jianqiang Vet Res Short Report In this study, we identified a chicken liver cell line (LMH) which could strongly support the replication of ALV-J (Subgroup J of avian leukosis virus) with high viral titer. Notably, ALV-J was efficiently detected by ELISA in LMH cells 1 day before DF1 cells. In comparison with DF1 cells, LMH cells not only expressed higher levels of ALV-J receptor chNHE-1, but also possessed a more efficient protein expression system for foreign genes. Thus, LMH cells could be a novel tool to shorten the ALV-J eradication approach and accelerate studies on the pathogenesis and oncogenesis of ALV-J. BioMed Central 2018-05-02 2018 /pmc/articles/PMC5932828/ /pubmed/29720272 http://dx.doi.org/10.1186/s13567-018-0537-7 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Li, Tuofan
Xie, Jing
Lv, Lu
Sun, Shu
Dong, Xiaomei
Xie, Quan
Liang, Guangcheng
Xia, Chichao
Shao, Hongxia
Qin, Aijian
Ye, Jianqiang
A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system
title A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system
title_full A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system
title_fullStr A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system
title_full_unstemmed A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system
title_short A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system
title_sort chicken liver cell line efficiently supports the replication of alv-j possibly through its high level viral receptor and efficient protein expression system
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932828/
https://www.ncbi.nlm.nih.gov/pubmed/29720272
http://dx.doi.org/10.1186/s13567-018-0537-7
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