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Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism
OBJECTIVE: Signal transduction not only initiates entry into the cell cycle, but also reprograms the cell’s metabolism. To control abnormalities in cell proliferation, both the aspects should be taken care of, thus pleiotropic signaling molecules are considered as crucial modulators. Considering thi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932847/ https://www.ncbi.nlm.nih.gov/pubmed/29720254 http://dx.doi.org/10.1186/s13104-018-3364-z |
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author | Gupta, Nutan Duggal, Shweta Kumar, Ajay Saquib, Najmuddin Mohd Rao, Kanury V. S. |
author_facet | Gupta, Nutan Duggal, Shweta Kumar, Ajay Saquib, Najmuddin Mohd Rao, Kanury V. S. |
author_sort | Gupta, Nutan |
collection | PubMed |
description | OBJECTIVE: Signal transduction not only initiates entry into the cell cycle, but also reprograms the cell’s metabolism. To control abnormalities in cell proliferation, both the aspects should be taken care of, thus pleiotropic signaling molecules are considered as crucial modulators. Considering this, we investigated the role of AKT1 in central carbon metabolism. The role of AKT1 has already been established in the process of cell cycle, but its contribution to the central carbon metabolism is sparsely studied. RESULTS: To address this, we combined the metabolomics and proteomics approaches. In accordance to our hypothesis, we found that the AKT1 kinase activity is regulating the levels of acetyl CoA through pyruvate dehydrogenase complex. Further, the decreased levels of acetyl CoA and dependency of acetyl CoA acetyl transferase protein on AKT1 kinase activity was also found to perturb the synthesis rate of palmitic acid which is a representative of fatty acid. This was analyzed in the present study using lipid labeling method through mass spectrometry. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3364-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5932847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59328472018-05-09 Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism Gupta, Nutan Duggal, Shweta Kumar, Ajay Saquib, Najmuddin Mohd Rao, Kanury V. S. BMC Res Notes Research Note OBJECTIVE: Signal transduction not only initiates entry into the cell cycle, but also reprograms the cell’s metabolism. To control abnormalities in cell proliferation, both the aspects should be taken care of, thus pleiotropic signaling molecules are considered as crucial modulators. Considering this, we investigated the role of AKT1 in central carbon metabolism. The role of AKT1 has already been established in the process of cell cycle, but its contribution to the central carbon metabolism is sparsely studied. RESULTS: To address this, we combined the metabolomics and proteomics approaches. In accordance to our hypothesis, we found that the AKT1 kinase activity is regulating the levels of acetyl CoA through pyruvate dehydrogenase complex. Further, the decreased levels of acetyl CoA and dependency of acetyl CoA acetyl transferase protein on AKT1 kinase activity was also found to perturb the synthesis rate of palmitic acid which is a representative of fatty acid. This was analyzed in the present study using lipid labeling method through mass spectrometry. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3364-z) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-02 /pmc/articles/PMC5932847/ /pubmed/29720254 http://dx.doi.org/10.1186/s13104-018-3364-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Note Gupta, Nutan Duggal, Shweta Kumar, Ajay Saquib, Najmuddin Mohd Rao, Kanury V. S. Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism |
title | Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism |
title_full | Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism |
title_fullStr | Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism |
title_full_unstemmed | Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism |
title_short | Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism |
title_sort | concurrent interactome and metabolome analysis reveals role of akt1 in central carbon metabolism |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932847/ https://www.ncbi.nlm.nih.gov/pubmed/29720254 http://dx.doi.org/10.1186/s13104-018-3364-z |
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