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Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods
In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of Weissella cibaria, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family Siphoviridae. Genomic analysis of WCP30 phage...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Food Science of Animal Resources
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932951/ https://www.ncbi.nlm.nih.gov/pubmed/29725210 http://dx.doi.org/10.5851/kosfa.2017.37.6.884 |
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author | Lee, Young-Duck Park, Jong-Hyun |
author_facet | Lee, Young-Duck Park, Jong-Hyun |
author_sort | Lee, Young-Duck |
collection | PubMed |
description | In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of Weissella cibaria, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family Siphoviridae. Genomic analysis of WCP30 phage showed that it had a 33,697-bp double-stranded DNA genome with 41.2% G+C content. Bioinformatics analysis of the genome revealed 35 open reading frames. A BLASTN search showed that WCP30 phage had low sequence similarity compared to other phages infecting lactic acid bacteria. This is the first report of the morphological features and complete genome sequence of WCP30 phage, which may be useful for controlling the fermentation of dairy foods. |
format | Online Article Text |
id | pubmed-5932951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Korean Society for Food Science of Animal Resources |
record_format | MEDLINE/PubMed |
spelling | pubmed-59329512018-05-03 Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods Lee, Young-Duck Park, Jong-Hyun Korean J Food Sci Anim Resour Article In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of Weissella cibaria, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family Siphoviridae. Genomic analysis of WCP30 phage showed that it had a 33,697-bp double-stranded DNA genome with 41.2% G+C content. Bioinformatics analysis of the genome revealed 35 open reading frames. A BLASTN search showed that WCP30 phage had low sequence similarity compared to other phages infecting lactic acid bacteria. This is the first report of the morphological features and complete genome sequence of WCP30 phage, which may be useful for controlling the fermentation of dairy foods. Korean Society for Food Science of Animal Resources 2017 2017-12-31 /pmc/articles/PMC5932951/ /pubmed/29725210 http://dx.doi.org/10.5851/kosfa.2017.37.6.884 Text en Copyright © 2017, Korean Society for Food Science of Animal Resources http://creativecommons.org/licences/by-nc/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licences/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Lee, Young-Duck Park, Jong-Hyun Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods |
title | Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods |
title_full | Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods |
title_fullStr | Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods |
title_full_unstemmed | Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods |
title_short | Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods |
title_sort | genomic analysis of wcp30 phage of weissella cibaria for dairy fermented foods |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932951/ https://www.ncbi.nlm.nih.gov/pubmed/29725210 http://dx.doi.org/10.5851/kosfa.2017.37.6.884 |
work_keys_str_mv | AT leeyoungduck genomicanalysisofwcp30phageofweissellacibariafordairyfermentedfoods AT parkjonghyun genomicanalysisofwcp30phageofweissellacibariafordairyfermentedfoods |