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Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods

In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of Weissella cibaria, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family Siphoviridae. Genomic analysis of WCP30 phage...

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Detalles Bibliográficos
Autores principales: Lee, Young-Duck, Park, Jong-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Food Science of Animal Resources 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932951/
https://www.ncbi.nlm.nih.gov/pubmed/29725210
http://dx.doi.org/10.5851/kosfa.2017.37.6.884
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author Lee, Young-Duck
Park, Jong-Hyun
author_facet Lee, Young-Duck
Park, Jong-Hyun
author_sort Lee, Young-Duck
collection PubMed
description In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of Weissella cibaria, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family Siphoviridae. Genomic analysis of WCP30 phage showed that it had a 33,697-bp double-stranded DNA genome with 41.2% G+C content. Bioinformatics analysis of the genome revealed 35 open reading frames. A BLASTN search showed that WCP30 phage had low sequence similarity compared to other phages infecting lactic acid bacteria. This is the first report of the morphological features and complete genome sequence of WCP30 phage, which may be useful for controlling the fermentation of dairy foods.
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spelling pubmed-59329512018-05-03 Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods Lee, Young-Duck Park, Jong-Hyun Korean J Food Sci Anim Resour Article In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of Weissella cibaria, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family Siphoviridae. Genomic analysis of WCP30 phage showed that it had a 33,697-bp double-stranded DNA genome with 41.2% G+C content. Bioinformatics analysis of the genome revealed 35 open reading frames. A BLASTN search showed that WCP30 phage had low sequence similarity compared to other phages infecting lactic acid bacteria. This is the first report of the morphological features and complete genome sequence of WCP30 phage, which may be useful for controlling the fermentation of dairy foods. Korean Society for Food Science of Animal Resources 2017 2017-12-31 /pmc/articles/PMC5932951/ /pubmed/29725210 http://dx.doi.org/10.5851/kosfa.2017.37.6.884 Text en Copyright © 2017, Korean Society for Food Science of Animal Resources http://creativecommons.org/licences/by-nc/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licences/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Lee, Young-Duck
Park, Jong-Hyun
Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods
title Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods
title_full Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods
title_fullStr Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods
title_full_unstemmed Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods
title_short Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods
title_sort genomic analysis of wcp30 phage of weissella cibaria for dairy fermented foods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932951/
https://www.ncbi.nlm.nih.gov/pubmed/29725210
http://dx.doi.org/10.5851/kosfa.2017.37.6.884
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