Cargando…
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
Chromosome mis-segregation leads to aneuploidy, a condition in which cells harbor an imbalanced chromosome number. Several lines of evidence strongly indicate that aneuploidy triggers genome instability, ultimately generating cells with complex karyotypes that arrest their proliferation. Isolation a...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5933501/ https://www.ncbi.nlm.nih.gov/pubmed/29708530 http://dx.doi.org/10.3791/57215 |
_version_ | 1783319971891249152 |
---|---|
author | Wang, Ruoxi W. MacDuffie, Emily Santaguida, Stefano |
author_facet | Wang, Ruoxi W. MacDuffie, Emily Santaguida, Stefano |
author_sort | Wang, Ruoxi W. |
collection | PubMed |
description | Chromosome mis-segregation leads to aneuploidy, a condition in which cells harbor an imbalanced chromosome number. Several lines of evidence strongly indicate that aneuploidy triggers genome instability, ultimately generating cells with complex karyotypes that arrest their proliferation. Isolation and characterization of cells harboring complex karyotypes are crucial to study the impact of an imbalanced chromosome number on cell physiology. To date, no methods have been established to reliably isolate such aneuploid cells. This paper provides a protocol for the enrichment and analysis of aneuploid cells with complex karyotypes utilizing standard, inexpensive tissue culture techniques. This protocol can be used to analyze several features of aneuploid cells with complex karyotypes including their induced senescence-associated secretory phenotype, pro-inflammatory properties, and ability to interact with immune cells. Because cancer cells often harbor imbalances in chromosome number, it is crucial to decipher how aneuploidy impacts cell physiology in normal cells, with the ultimate goal of uncovering both its pro- and anti-tumorigenic effects. |
format | Online Article Text |
id | pubmed-5933501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-59335012018-05-16 Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes Wang, Ruoxi W. MacDuffie, Emily Santaguida, Stefano J Vis Exp Cancer Research Chromosome mis-segregation leads to aneuploidy, a condition in which cells harbor an imbalanced chromosome number. Several lines of evidence strongly indicate that aneuploidy triggers genome instability, ultimately generating cells with complex karyotypes that arrest their proliferation. Isolation and characterization of cells harboring complex karyotypes are crucial to study the impact of an imbalanced chromosome number on cell physiology. To date, no methods have been established to reliably isolate such aneuploid cells. This paper provides a protocol for the enrichment and analysis of aneuploid cells with complex karyotypes utilizing standard, inexpensive tissue culture techniques. This protocol can be used to analyze several features of aneuploid cells with complex karyotypes including their induced senescence-associated secretory phenotype, pro-inflammatory properties, and ability to interact with immune cells. Because cancer cells often harbor imbalances in chromosome number, it is crucial to decipher how aneuploidy impacts cell physiology in normal cells, with the ultimate goal of uncovering both its pro- and anti-tumorigenic effects. MyJove Corporation 2018-04-13 /pmc/articles/PMC5933501/ /pubmed/29708530 http://dx.doi.org/10.3791/57215 Text en Copyright © 2018, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Cancer Research Wang, Ruoxi W. MacDuffie, Emily Santaguida, Stefano Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
title | Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
title_full | Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
title_fullStr | Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
title_full_unstemmed | Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
title_short | Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
title_sort | generation and isolation of cell cycle-arrested cells with complex karyotypes |
topic | Cancer Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5933501/ https://www.ncbi.nlm.nih.gov/pubmed/29708530 http://dx.doi.org/10.3791/57215 |
work_keys_str_mv | AT wangruoxiw generationandisolationofcellcyclearrestedcellswithcomplexkaryotypes AT macduffieemily generationandisolationofcellcyclearrestedcellswithcomplexkaryotypes AT santaguidastefano generationandisolationofcellcyclearrestedcellswithcomplexkaryotypes |