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Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans

Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals. For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction. By adding h...

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Detalles Bibliográficos
Autores principales:	Kameyama, Akihiko, Dissanayake, Santha Kumara, Thet Tin, Wai Wai
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5933716/ https://www.ncbi.nlm.nih.gov/pubmed/29723274 http://dx.doi.org/10.1371/journal.pone.0196800

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