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Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits

NAC proteins are a family of transcription factors which have a variety of important regulatory roles in plants. They present a very well conserved group of NAC subdomains in the N-terminal region and a highly variable domain at the C-terminus. Currently, knowledge concerning NAC family in the straw...

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Autores principales: Moyano, Enriqueta, Martínez-Rivas, Félix J., Blanco-Portales, Rosario, Molina-Hidalgo, Francisco Javier, Ric-Varas, Pablo, Matas-Arroyo, Antonio J., Caballero, José Luis, Muñoz-Blanco, Juan, Rodríguez-Franco, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5933797/
https://www.ncbi.nlm.nih.gov/pubmed/29723301
http://dx.doi.org/10.1371/journal.pone.0196953
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author Moyano, Enriqueta
Martínez-Rivas, Félix J.
Blanco-Portales, Rosario
Molina-Hidalgo, Francisco Javier
Ric-Varas, Pablo
Matas-Arroyo, Antonio J.
Caballero, José Luis
Muñoz-Blanco, Juan
Rodríguez-Franco, Antonio
author_facet Moyano, Enriqueta
Martínez-Rivas, Félix J.
Blanco-Portales, Rosario
Molina-Hidalgo, Francisco Javier
Ric-Varas, Pablo
Matas-Arroyo, Antonio J.
Caballero, José Luis
Muñoz-Blanco, Juan
Rodríguez-Franco, Antonio
author_sort Moyano, Enriqueta
collection PubMed
description NAC proteins are a family of transcription factors which have a variety of important regulatory roles in plants. They present a very well conserved group of NAC subdomains in the N-terminal region and a highly variable domain at the C-terminus. Currently, knowledge concerning NAC family in the strawberry plant remains very limited. In this work, we analyzed the NAC family of Fragaria vesca, and a total of 112 NAC proteins were identified after we curated the annotations from the version 4.0.a1 genome. They were placed into the ligation groups (pseudo-chromosomes) and described its physicochemical and genetic features. A microarray transcriptomic analysis showed six of them expressed during the development and ripening of the Fragaria x ananassa fruit. Their expression patterns were studied in fruit (receptacle and achenes) in different stages of development and in vegetative tissues. Also, the expression level under different hormonal treatments (auxins, ABA) and drought stress was investigated. In addition, they were clustered with other NAC transcription factor with known function related to growth and development, senescence, fruit ripening, stress response, and secondary cell wall and vascular development. Our results indicate that these six strawberry NAC proteins could play different important regulatory roles in the process of development and ripening of the fruit, providing the basis for further functional studies and the selection for NAC candidates suitable for biotechnological applications.
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spelling pubmed-59337972018-05-18 Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits Moyano, Enriqueta Martínez-Rivas, Félix J. Blanco-Portales, Rosario Molina-Hidalgo, Francisco Javier Ric-Varas, Pablo Matas-Arroyo, Antonio J. Caballero, José Luis Muñoz-Blanco, Juan Rodríguez-Franco, Antonio PLoS One Research Article NAC proteins are a family of transcription factors which have a variety of important regulatory roles in plants. They present a very well conserved group of NAC subdomains in the N-terminal region and a highly variable domain at the C-terminus. Currently, knowledge concerning NAC family in the strawberry plant remains very limited. In this work, we analyzed the NAC family of Fragaria vesca, and a total of 112 NAC proteins were identified after we curated the annotations from the version 4.0.a1 genome. They were placed into the ligation groups (pseudo-chromosomes) and described its physicochemical and genetic features. A microarray transcriptomic analysis showed six of them expressed during the development and ripening of the Fragaria x ananassa fruit. Their expression patterns were studied in fruit (receptacle and achenes) in different stages of development and in vegetative tissues. Also, the expression level under different hormonal treatments (auxins, ABA) and drought stress was investigated. In addition, they were clustered with other NAC transcription factor with known function related to growth and development, senescence, fruit ripening, stress response, and secondary cell wall and vascular development. Our results indicate that these six strawberry NAC proteins could play different important regulatory roles in the process of development and ripening of the fruit, providing the basis for further functional studies and the selection for NAC candidates suitable for biotechnological applications. Public Library of Science 2018-05-03 /pmc/articles/PMC5933797/ /pubmed/29723301 http://dx.doi.org/10.1371/journal.pone.0196953 Text en © 2018 Moyano et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Moyano, Enriqueta
Martínez-Rivas, Félix J.
Blanco-Portales, Rosario
Molina-Hidalgo, Francisco Javier
Ric-Varas, Pablo
Matas-Arroyo, Antonio J.
Caballero, José Luis
Muñoz-Blanco, Juan
Rodríguez-Franco, Antonio
Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits
title Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits
title_full Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits
title_fullStr Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits
title_full_unstemmed Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits
title_short Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits
title_sort genome-wide analysis of the nac transcription factor family and their expression during the development and ripening of the fragaria × ananassa fruits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5933797/
https://www.ncbi.nlm.nih.gov/pubmed/29723301
http://dx.doi.org/10.1371/journal.pone.0196953
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