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Development of a generic β-lactamase screening system for improved signal peptides for periplasmic targeting of recombinant proteins in Escherichia coli

Targeting of recombinant proteins to the Escherichia coli periplasm is a desirable industrial processing tool to allow formation of disulphide bonds, aid folding and simplify recovery. Proteins are targeted across the inner membrane to the periplasm by an N-terminal signal peptide. The sequence of t...

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Detalles Bibliográficos
Autores principales:	Selas Castiñeiras, Tania, Williams, Steven G., Hitchcock, Antony, Cole, Jeffrey A., Smith, Daniel C., Overton, Tim W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5934370/ https://www.ncbi.nlm.nih.gov/pubmed/29725125 http://dx.doi.org/10.1038/s41598-018-25192-3

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