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A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials
There is a growing necessity to acquire more profound knowledge on the quantity of eluates from resin-based dental materials, especially with regard to bisphenol A (BPA). The aim of the present study was to develop a highly sensitive method to characterize the short-term release of BPA in saliva wit...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5934439/ https://www.ncbi.nlm.nih.gov/pubmed/29725047 http://dx.doi.org/10.1038/s41598-018-24815-z |
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author | De Nys, Siemon Putzeys, Eveline Vervliet, Philippe Covaci, Adrian Boonen, Imke Elskens, Marc Vanoirbeek, Jeroen Godderis, Lode Van Meerbeek, Bart Van Landuyt, Kirsten L. Duca, Radu Corneliu |
author_facet | De Nys, Siemon Putzeys, Eveline Vervliet, Philippe Covaci, Adrian Boonen, Imke Elskens, Marc Vanoirbeek, Jeroen Godderis, Lode Van Meerbeek, Bart Van Landuyt, Kirsten L. Duca, Radu Corneliu |
author_sort | De Nys, Siemon |
collection | PubMed |
description | There is a growing necessity to acquire more profound knowledge on the quantity of eluates from resin-based dental materials, especially with regard to bisphenol A (BPA). The aim of the present study was to develop a highly sensitive method to characterize the short-term release of BPA in saliva with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), using an extraction step and additional derivatization of BPA with pyridine-3-sulfonyl chloride. Light-cured resin-based composites were incubated at 37 °C in 1 mL artificial saliva, which was refreshed daily for one week. The final protocol allows accurate quantification of very low levels of BPA in samples of artificial saliva (i.e. 1.10 pmol BPA/mL or 250 pg/mL). The daily BPA-release from dental composites, ranging from 1.10 to 7.46 pmol BPA/mL, was characterized over a period of 7 days. The highest total amount of BPA was released from Solitaire 2 (24.72 ± 2.86 pmol), followed by G-ænial Posterior (15.51 ± 0.88 pmol) and Filtek Supreme XTE (12.00 ± 1.31 pmol). In contrast, only trace amounts of BPA were released from Ceram.x Universal. This UPLC-MS/MS method might be used for clinical research focusing on the evaluation of the clinical relevance of BPA release from dental materials. |
format | Online Article Text |
id | pubmed-5934439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59344392018-05-10 A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials De Nys, Siemon Putzeys, Eveline Vervliet, Philippe Covaci, Adrian Boonen, Imke Elskens, Marc Vanoirbeek, Jeroen Godderis, Lode Van Meerbeek, Bart Van Landuyt, Kirsten L. Duca, Radu Corneliu Sci Rep Article There is a growing necessity to acquire more profound knowledge on the quantity of eluates from resin-based dental materials, especially with regard to bisphenol A (BPA). The aim of the present study was to develop a highly sensitive method to characterize the short-term release of BPA in saliva with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), using an extraction step and additional derivatization of BPA with pyridine-3-sulfonyl chloride. Light-cured resin-based composites were incubated at 37 °C in 1 mL artificial saliva, which was refreshed daily for one week. The final protocol allows accurate quantification of very low levels of BPA in samples of artificial saliva (i.e. 1.10 pmol BPA/mL or 250 pg/mL). The daily BPA-release from dental composites, ranging from 1.10 to 7.46 pmol BPA/mL, was characterized over a period of 7 days. The highest total amount of BPA was released from Solitaire 2 (24.72 ± 2.86 pmol), followed by G-ænial Posterior (15.51 ± 0.88 pmol) and Filtek Supreme XTE (12.00 ± 1.31 pmol). In contrast, only trace amounts of BPA were released from Ceram.x Universal. This UPLC-MS/MS method might be used for clinical research focusing on the evaluation of the clinical relevance of BPA release from dental materials. Nature Publishing Group UK 2018-05-03 /pmc/articles/PMC5934439/ /pubmed/29725047 http://dx.doi.org/10.1038/s41598-018-24815-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article De Nys, Siemon Putzeys, Eveline Vervliet, Philippe Covaci, Adrian Boonen, Imke Elskens, Marc Vanoirbeek, Jeroen Godderis, Lode Van Meerbeek, Bart Van Landuyt, Kirsten L. Duca, Radu Corneliu A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials |
title | A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials |
title_full | A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials |
title_fullStr | A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials |
title_full_unstemmed | A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials |
title_short | A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials |
title_sort | novel high sensitivity uplc-ms/ms method for the evaluation of bisphenol a leaching from dental materials |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5934439/ https://www.ncbi.nlm.nih.gov/pubmed/29725047 http://dx.doi.org/10.1038/s41598-018-24815-z |
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