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Microfluidic PCR Amplification and MiSeq Amplicon Sequencing Techniques for High-Throughput Detection and Genotyping of Human Pathogenic RNA Viruses in Human Feces, Sewage, and Oysters

Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed to develop a high...

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Detalles Bibliográficos
Autores principales:	Oshiki, Mamoru, Miura, Takayuki, Kazama, Shinobu, Segawa, Takahiro, Ishii, Satoshi, Hatamoto, Masashi, Yamaguchi, Takashi, Kubota, Kengo, Iguchi, Akinori, Tagawa, Tadashi, Okubo, Tsutomu, Uemura, Shigeki, Harada, Hideki, Kobayashi, Naohiro, Araki, Nobuo, Sano, Daisuke
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2018
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5934477/ https://www.ncbi.nlm.nih.gov/pubmed/29755444 http://dx.doi.org/10.3389/fmicb.2018.00830

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