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Primed CRISPR adaptation in Escherichia coli cells does not depend on conformational changes in the Cascade effector complex detected in Vitro

In type I CRISPR–Cas systems, primed adaptation of new spacers into CRISPR arrays occurs when the effector Cascade–crRNA complex recognizes imperfectly matched targets that are not subject to efficient CRISPR interference. Thus, primed adaptation allows cells to acquire additional protection against...

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Detalles Bibliográficos
Autores principales:	Krivoy, Andrey, Rutkauskas, Marius, Kuznedelov, Konstantin, Musharova, Olga, Rouillon, Christophe, Severinov, Konstantin, Seidel, Ralf
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5934681/ https://www.ncbi.nlm.nih.gov/pubmed/29596641 http://dx.doi.org/10.1093/nar/gky219

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