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Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera
The white-backed planthopper, Sogatella furcifera, is a phloem sap feeder that secretes watery and gelling saliva during feeding. In this study, we identified the major proteins in watery saliva of S. furcifera by shotgun LC-MS/MS analysis combined with transcriptomic analysis. A total of 161 protei...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935387/ https://www.ncbi.nlm.nih.gov/pubmed/29727440 http://dx.doi.org/10.1371/journal.pone.0193831 |
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author | Miao, Yu-Tong Deng, Yao Jia, Hao-Kang Liu, Yu-Di Hou, Mao-Lin |
author_facet | Miao, Yu-Tong Deng, Yao Jia, Hao-Kang Liu, Yu-Di Hou, Mao-Lin |
author_sort | Miao, Yu-Tong |
collection | PubMed |
description | The white-backed planthopper, Sogatella furcifera, is a phloem sap feeder that secretes watery and gelling saliva during feeding. In this study, we identified the major proteins in watery saliva of S. furcifera by shotgun LC-MS/MS analysis combined with transcriptomic analysis. A total of 161 proteins were identified, which were divided into 8 function categories, including enzymes, transporter, calcium ion binding protein, salivary sheath protein, cytoskeleton protein, DNA-, RNA-, and protein-binding or regulating proteins, other non-enzyme proteins and unknown proteins. Gene expression pattern of 11 secretory proteins were analyzed by real time quantitative-PCR. We detected the mucin-like protein, which had a unique expression level in salivary gland, most likely to be a candidate effector involved in regulation of plant defense. This study identified the watery saliva component of S. furcifera and it provided a list of proteins which may play a role in interaction between S. furcifera and rice. |
format | Online Article Text |
id | pubmed-5935387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59353872018-05-18 Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera Miao, Yu-Tong Deng, Yao Jia, Hao-Kang Liu, Yu-Di Hou, Mao-Lin PLoS One Research Article The white-backed planthopper, Sogatella furcifera, is a phloem sap feeder that secretes watery and gelling saliva during feeding. In this study, we identified the major proteins in watery saliva of S. furcifera by shotgun LC-MS/MS analysis combined with transcriptomic analysis. A total of 161 proteins were identified, which were divided into 8 function categories, including enzymes, transporter, calcium ion binding protein, salivary sheath protein, cytoskeleton protein, DNA-, RNA-, and protein-binding or regulating proteins, other non-enzyme proteins and unknown proteins. Gene expression pattern of 11 secretory proteins were analyzed by real time quantitative-PCR. We detected the mucin-like protein, which had a unique expression level in salivary gland, most likely to be a candidate effector involved in regulation of plant defense. This study identified the watery saliva component of S. furcifera and it provided a list of proteins which may play a role in interaction between S. furcifera and rice. Public Library of Science 2018-05-04 /pmc/articles/PMC5935387/ /pubmed/29727440 http://dx.doi.org/10.1371/journal.pone.0193831 Text en © 2018 Miao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Miao, Yu-Tong Deng, Yao Jia, Hao-Kang Liu, Yu-Di Hou, Mao-Lin Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera |
title | Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera |
title_full | Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera |
title_fullStr | Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera |
title_full_unstemmed | Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera |
title_short | Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera |
title_sort | proteomic analysis of watery saliva secreted by white-backed planthopper, sogatella furcifera |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935387/ https://www.ncbi.nlm.nih.gov/pubmed/29727440 http://dx.doi.org/10.1371/journal.pone.0193831 |
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