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MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells

Natriuretic Peptide Receptor 3 (NPR3), the clearance receptor for extracellular bio-active natriuretic peptides (NPs), plays important roles in the homeostasis of body fluid volume and vascular tone. Using luciferase reporter and antagomir-based silencing assays, we demonstrated that the expression...

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Autores principales: Wang, Juan, Tong, Kai Sing, Wong, Lee Lee, Liew, Oi-Wah, Raghuram, Divya, Richards, Arthur Mark, Chen, Yei-Tsung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935707/
https://www.ncbi.nlm.nih.gov/pubmed/29728596
http://dx.doi.org/10.1038/s41598-018-25489-3
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author Wang, Juan
Tong, Kai Sing
Wong, Lee Lee
Liew, Oi-Wah
Raghuram, Divya
Richards, Arthur Mark
Chen, Yei-Tsung
author_facet Wang, Juan
Tong, Kai Sing
Wong, Lee Lee
Liew, Oi-Wah
Raghuram, Divya
Richards, Arthur Mark
Chen, Yei-Tsung
author_sort Wang, Juan
collection PubMed
description Natriuretic Peptide Receptor 3 (NPR3), the clearance receptor for extracellular bio-active natriuretic peptides (NPs), plays important roles in the homeostasis of body fluid volume and vascular tone. Using luciferase reporter and antagomir-based silencing assays, we demonstrated that the expression of NPR3 could be modulated by microRNA-143 (miR-143-3p), a microRNA species with up-regulated circulating concentrations in clinical heart failure. The regulatory effect of miR-143 on NPR3 expression was further evidenced by the reciprocal relationship between miR-143 and NPR3 levels observed in hypoxia-treated human cardiac cells and in left ventricular tissue from rats undergoing experimental myocardial infarction. Further analysis indicated elevation of miR-143 in response to hypoxic challenge reflects transcriptional activation of the miR-143 host gene (MIR143HG). This was corroborated by demonstration of the induction of host gene promoter activity upon hypoxic challenge. Moreover, miR-143 was shown to enhance its own expression by increasing MIR143HG promoter activity, as well as targeting the expressions of NPPA, NPPC, NR3C2, and CRHR2 in cardiac cells. Taken together, these findings suggest that the elevation of miR-143 upon hypoxic insult may be part of a microRNA-based feed forward loop that results in fine tuning the levels of NPs and neurohormonal receptors in cardiac cell lineages.
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spelling pubmed-59357072018-05-10 MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells Wang, Juan Tong, Kai Sing Wong, Lee Lee Liew, Oi-Wah Raghuram, Divya Richards, Arthur Mark Chen, Yei-Tsung Sci Rep Article Natriuretic Peptide Receptor 3 (NPR3), the clearance receptor for extracellular bio-active natriuretic peptides (NPs), plays important roles in the homeostasis of body fluid volume and vascular tone. Using luciferase reporter and antagomir-based silencing assays, we demonstrated that the expression of NPR3 could be modulated by microRNA-143 (miR-143-3p), a microRNA species with up-regulated circulating concentrations in clinical heart failure. The regulatory effect of miR-143 on NPR3 expression was further evidenced by the reciprocal relationship between miR-143 and NPR3 levels observed in hypoxia-treated human cardiac cells and in left ventricular tissue from rats undergoing experimental myocardial infarction. Further analysis indicated elevation of miR-143 in response to hypoxic challenge reflects transcriptional activation of the miR-143 host gene (MIR143HG). This was corroborated by demonstration of the induction of host gene promoter activity upon hypoxic challenge. Moreover, miR-143 was shown to enhance its own expression by increasing MIR143HG promoter activity, as well as targeting the expressions of NPPA, NPPC, NR3C2, and CRHR2 in cardiac cells. Taken together, these findings suggest that the elevation of miR-143 upon hypoxic insult may be part of a microRNA-based feed forward loop that results in fine tuning the levels of NPs and neurohormonal receptors in cardiac cell lineages. Nature Publishing Group UK 2018-05-04 /pmc/articles/PMC5935707/ /pubmed/29728596 http://dx.doi.org/10.1038/s41598-018-25489-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Juan
Tong, Kai Sing
Wong, Lee Lee
Liew, Oi-Wah
Raghuram, Divya
Richards, Arthur Mark
Chen, Yei-Tsung
MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title_full MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title_fullStr MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title_full_unstemmed MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title_short MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title_sort microrna-143 modulates the expression of natriuretic peptide receptor 3 in cardiac cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935707/
https://www.ncbi.nlm.nih.gov/pubmed/29728596
http://dx.doi.org/10.1038/s41598-018-25489-3
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