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Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat

Plants deploy several ammonium transporter (AMT) and nitrate transporter (NRT) genes to acquire NH(4)(+) and NO(3)(−) from the soil into the roots and then transport them to other plant organs. Coding sequences of wheat genes obtained from ENSEMBL were aligned to known AMT and NRT sequences of Arabi...

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Autores principales: Bajgain, Prabin, Russell, Blake, Mohammadi, Mohsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935732/
https://www.ncbi.nlm.nih.gov/pubmed/29728590
http://dx.doi.org/10.1038/s41598-018-25430-8
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author Bajgain, Prabin
Russell, Blake
Mohammadi, Mohsen
author_facet Bajgain, Prabin
Russell, Blake
Mohammadi, Mohsen
author_sort Bajgain, Prabin
collection PubMed
description Plants deploy several ammonium transporter (AMT) and nitrate transporter (NRT) genes to acquire NH(4)(+) and NO(3)(−) from the soil into the roots and then transport them to other plant organs. Coding sequences of wheat genes obtained from ENSEMBL were aligned to known AMT and NRT sequences of Arabidopsis, barley, maize, rice, and wheat to retrieve homologous genes. Bayesian phylogenetic relationships among these genes showed distinct classification of sequences with significant homology to NRT1, NRT2, and NRT3 (NAR2). Inter-species gene duplication analysis showed that eight AMT and 77 NRT genes were orthologous to the AMT and NRT genes of aforementioned plant species. Expression patterns of these genes were studied via whole transcriptome sequencing of 21-day old seedlings of five spring wheat lines. Eight AMT and 52 NRT genes were differentially expressed between root and shoot; and 131 genes did not express neither in root nor in shoot of 21-day old seedlings. Homeologous genes in the A, B, and D genomes, characterized by high sequence homology, revealed that their counterparts exhibited different expression patterns. This complement and evolutionary relationship of wheat AMT and NRT genes is expected to help in development of wheat germplasm with increased efficiency in nitrogen uptake and usage.
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spelling pubmed-59357322018-05-10 Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat Bajgain, Prabin Russell, Blake Mohammadi, Mohsen Sci Rep Article Plants deploy several ammonium transporter (AMT) and nitrate transporter (NRT) genes to acquire NH(4)(+) and NO(3)(−) from the soil into the roots and then transport them to other plant organs. Coding sequences of wheat genes obtained from ENSEMBL were aligned to known AMT and NRT sequences of Arabidopsis, barley, maize, rice, and wheat to retrieve homologous genes. Bayesian phylogenetic relationships among these genes showed distinct classification of sequences with significant homology to NRT1, NRT2, and NRT3 (NAR2). Inter-species gene duplication analysis showed that eight AMT and 77 NRT genes were orthologous to the AMT and NRT genes of aforementioned plant species. Expression patterns of these genes were studied via whole transcriptome sequencing of 21-day old seedlings of five spring wheat lines. Eight AMT and 52 NRT genes were differentially expressed between root and shoot; and 131 genes did not express neither in root nor in shoot of 21-day old seedlings. Homeologous genes in the A, B, and D genomes, characterized by high sequence homology, revealed that their counterparts exhibited different expression patterns. This complement and evolutionary relationship of wheat AMT and NRT genes is expected to help in development of wheat germplasm with increased efficiency in nitrogen uptake and usage. Nature Publishing Group UK 2018-05-04 /pmc/articles/PMC5935732/ /pubmed/29728590 http://dx.doi.org/10.1038/s41598-018-25430-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bajgain, Prabin
Russell, Blake
Mohammadi, Mohsen
Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title_full Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title_fullStr Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title_full_unstemmed Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title_short Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title_sort phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935732/
https://www.ncbi.nlm.nih.gov/pubmed/29728590
http://dx.doi.org/10.1038/s41598-018-25430-8
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